Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of >200 IU/mg.
Alternative Names
Cartilage derived retinoic acid sensitive protein; CD RAP; CDRAP; Melanoma derived growth regulatory protein precursor; Melanoma inhibitory activity protein; Melanoma-derived growth regulatory protein; Mia; MIA_HUMAN
Species
Homo sapiens (Human)
Expression Region
25-131aa
Complete Sequence
GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ
Protein Length
Full Length of Mature Protein
Form
Liquid or Lyophilized powder
Buffer
0.2 µm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose,lyophilized
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Datasheet & COA
Please contact us to get it.
Description
The Recombinant Human MIA is a full-length, melanoma-derived growth regulatory protein encompassing the 25-131aa expression region. Manufactured in E. Coli, this protein is vital for immunology research. As a tag-free protein, MIA exhibits a purity of over 98%, as verified by SDS-PAGE and HPLC. Endotoxin levels are maintained below 1.0 EU/µg, as determined using the LAL method. MIA is fully biologically active, with an ED50 value of less than 5 μg/mL in a cell proliferation assay involving the human A375 cell line, indicating a specific activity of more than 200 IU/mg. The lyophilized powder form allows for straightforward storage and usage in a range of research applications.
