Rat TNF-α ELISA kit

This ELISA kit provides quantitative measurement of TNF (tumor necrosis factor) in Rattus norvegicus samples, supporting immunology research focused on innate immune activation and inflammatory signaling. Because TNF is a rapidly induced, short-lived cytokine whose concentrations can vary dramatically between baseline and inflammatory states, this kit is designed to capture that full dynamic window across multiple sample contexts.

Detection Range: The 15.6–1000 pg/mL range spans from near-baseline circulating TNF levels in unstimulated rat tissues to the elevated concentrations observed in LPS-challenged or sepsis-model animals, minimizing the need for serial dilutions while covering both resting and acutely inflamed conditions.

Sensitivity: At 3.9 pg/mL, the kit detects TNF in samples where expression is low — such as unstimulated macrophage supernatants, early-phase inflammatory responses, or tissue homogenates from localized injury models — where TNF may be present but not yet amplified.

Sample Compatibility: Validation across tissue homogenates, cell culture supernatants, and bronchoalveolar lavage fluid enables researchers to connect organ-level TNF production, cellular secretion kinetics, and pulmonary compartment inflammation within a single experimental framework, critical for acute lung injury or pneumonia models.

Assay Efficiency: Requiring only 50–100 µL and 1–5 hours, the kit accommodates limited-volume BAL fluid collections and time-sensitive multi-cytokine profiling from primary rat macrophage cultures.

This kit directly supports studies in experimental sepsis, ventilator-induced lung injury, and rheumatoid arthritis modeling.