Rat Gastrin ELISA Kit

Instructions
Code CSB-E12743r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name gastrin
Alternative Names Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
Abbreviation GAST
Uniprot No. P04563
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates ,tissue homogenates
Detection Range 0.312 pg/mL-20 pg/mL
Sensitivity 0.078 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 85-95  
1:200 Average % 94  
Range % 90-98  
1:400 Average % 99  
Range % 95-104  
1:800 Average % 102  
Range % 98-107  
Recovery
The recovery of rat gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 100 95-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
20 2.496 2.537 2.517 2.390  
10 2.181 2.192 2.187 2.060  
5 1.795 1.741 1.768 1.641  
2.5 1.353 1.313 1.333 1.206  
1.25 0.878 0.898 0.888 0.761  
0.625 0.369 0.366 0.368 0.241  
0.312 0.242 0.259 0.251 0.124  
0 0.123 0.130 0.127    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Gene References into Functions
  1. we examined the chronic effects of a GLP-1-gastrin dual agonist ZP3022 on glycemic control and beta cell dysfunction in overtly diabetic Zucker Diabetic Fatty PMID: 25849341
  2. treatment with gastrin, a CCK2R agonist, stimulated the secretion of GLP-1, and that this effect was likely due to increased expression of proglucagon and PCSK1 (also known as prohormone convertase 3 (PC3 gene)). PMID: 25601282
  3. The results suggested that clusterin may contribute to gastrin's growth-promoting effect on the oxyntic mucosa. PMID: 21995960
  4. plays a role in the development of gastric mucosa, ECL cells and A-like cells in newborn and young rats PMID: 12220729
  5. role in increasing expression of COX-2 mRNA and protein, the activity of the COX-2 promoter, and the release of prostaglandin E(2) PMID: 12239223
  6. High temperature can affect gastrin, somatostatin and motilin production in the gastric antral mucosa of rats with gastric ulcer. PMID: 12376287
  7. study demonstrates that gastrin enhances gastric mucosal integrity through cyclooxygenase-2 PMID: 12388195
  8. Data suggest that distention of isolated rat stomach stimulates somatostatin release via cholinergic and non-cholinergic TTX-insensitive pathways, and that these pathways are responsible for distention-induced inhibition of gastrin release. PMID: 12508367
  9. gastrin induces Akt through activation of CCKB receptors and of intracellular calcium-dependent, PKC-independent, pathways PMID: 15351698
  10. the role of JAK-dependent pathways in the regulation of E-cadherin-dependent cell-cell adhesion by gastrin PMID: 15530849
  11. gastrin- and PACAP-stimulated secretion of histamine and pancreastatin is inhibited by somatostatin, misoprostol and galanin in enterochromaffin-like cells PMID: 15935492
  12. hypogastrinemia and impaired enterochromaffin cell function in the oxyntic mucosa of the stomach might be partially responsible for the reduction in body weight that occurs after gastric bypass PMID: 16645284
  13. G cells are luminal sampling chemomechanosensory cells whose gastrin secretion is regulated by neural, hormonal, luminal, and mechanical factors through protein kinase A activation, cAMP signaling, and mitogen-activated protein kinase phosphorylation. PMID: 19208342

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Subcellular Location Secreted
Protein Families Gastrin/cholecystokinin family
Database Links

KEGG: rno:25320

STRING: 10116.ENSRNOP00000019863

UniGene: Rn.9754

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