The Rat Immunoglobulin G (IgG) ELISA Kit is specifically designed to standardize the detection of rat IgG in serum and plasma. This kit employs the Competitive-ELISA mechanism to quantitatively measure the concentration of IgG. The rat IgG has been pre-coated on the microtiter plate. Standards and samples are pipetted into the wells with HRP-conjugated antibody specific for rat IgG. A competitive inhibition reaction is launched between IgG (standards or samples) and pre-coated IgG with HRP-conjugated IgG antibody. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells. HRP-TMB interaction elicits a chromogenic reaction. The color development is terminated, and the solution color changes from blue to yellow. The color intensity is in opposition to the amount of rat IgG in samples. This kit has been professionally verified with reliable quality, high sensitivity, excellent specificity, good linearity, precision less than 10%, and consistency between batches. See more details on the product instructions.
IgG is the primary type of antibody found in blood circulation extracellular fluids. It has four isoforms, including IgG1, IgG2, IgG3, and IgG4. It is produced and released by plasma B cells in response to an antigen. IgG has four isoforms: IgG1-4. Each IgG has two antigen-binding sites. It protects the body from infections by binding to a variety of pathogens such as fungi, viruses, and bacteria. IgG is the sole Ig subclass that can cross the placenta in humans, and it largely protects the newborn during the first months of life. IgG's relative abundance and excellent specificity to antigens make it become the principal antibody applied in immunological research and clinical diagnostics. The measurement of IgG can be used as a diagnostic tool for certain conditions, such as autoimmune hepatitis if indicated by certain symptoms. And IgG antibodies also play a role in the diagnosis of food allergy.