Rat Immunoglobulin G,IgG ELISA Kit

Instructions
Code CSB-E07981r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Immunoglobulin G,IgG
Alternative Names N/A
Abbreviation IgG
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 31.25 ng/mL-8000 ng/mL
Sensitivity 31.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 100  
Range % 95-104  
1:2000 Average % 90  
Range % 86-95  
1:4000 Average % 87  
Range % 83-92  
1:8000 Average % 101  
Range % 97-106  
Recovery
The recovery of rat IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 88-95  
EDTA plasma (n=4) 93 89-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
8000 0.230 0.225 0.228    
2000 0.417 0.407 0.412    
500 0.643 0.664 0.654    
125 1.238 1.257 1.248    
31.25 1.754 1.770 1.762    
0 2.035 1.988 2.012    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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