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- A 96-well Assay plate --The 96-well plate has been pre-coated with rat IgG.
- Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- HRP-conjugated IgG(1 x 6 ml) --Bind to the IgG, and HRP catalyzes the TMB to elicit a chromogenic reaction.
- Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (25x concentrate) 1 x 20 ml --Wash away unbound or free substances.
- TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
