Code | CSB-RP185374Ba |
Abbreviation | Recombinant E.coli fre protein |
MSDS | |
Size | $388 |
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The expression of recombinant Escherichia coli (strain K12) fre protein in e.coli cells requires incorporating a DNA fragment encoding the Escherichia coli (strain K12) fre protein (2-233aa) into a plasmid vector and transforming this vector into e.coli cells. Positive cells are screened, cultured, and induced to produce the fre protein. A N-terminal 6xHis tag is attached to the protein. The recombinant Escherichia coli (strain K12) fre protein is collected and purified from the cell lysate through affinity purification. It is assessed through SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue, with a purity of exceeding 90%.
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KEGG: ecj:JW3820
STRING: 316385.ECDH10B_4033