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Mouse Noggin(NOG) ELISA kit

Mouse Noggin(NOG) ELISA kit

Specification
Target

Code

CSB-EL015917MO

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P97466

Lead Time

5-7 working days

Abbreviation

NOG

Protein Biological Process 1

Developmental Protein

Target Name

noggin

Alias

SYM1, SYNS1, symphalangism 1 (proximal)

Species

Mus musculus (Mouse)

Protein Biological Process 3

Chondrogenesis

Sample Types

serum, plasma, tissue homogenates, cell culture supernates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Developmental Biology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.

HGNC

21535

RGD

620783

MGI

1916487

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	95
	Range %	90-97
1:10	Average %	89
	Range %	81-99
1:20	Average %	95
	Range %	87-100
1:40	Average %	93
	Range %	85-104

Recovery

The recovery of mouse NOG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-99
EDTA plasma (n=4)	97	83-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.601	2.593	2.597	2.505
1000	1.454	1.496	1.475	1.383
500	0.795	0.807	0.801	0.709
250	0.432	0.426	0.429	0.337
125	0.274	0.288	0.281	0.189
62.5	0.181	0.175	0.178	0.086
31.25	0.131	0.128	0.130	0.038
0	0.092	0.091	0.092

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Function	Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite
Subcellular Location	Secreted
Protein Families	Noggin family
Tissue Specificity	Expressed in condensing cartilage and immature chondrocytes.
Database Links	KEGG: mmu:18121 STRING: 10090.ENSMUSP00000061427 UniGene: Mm.135266
Pathway	TGF-beta signaling pathway

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CSB-YP015917MO CSB-EP015917MO CSB-BP015917MO CSB-MP015917MO	Recombinant Mouse Noggin(Nog)	Yeast E.coli Baculovirus Mammalian cell

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