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Recombinant Bacillus subtilis Sporulation kinase A (kinA), partial

Recombinant Bacillus subtilis Sporulation kinase A (kinA), partial

Code	CSB-YP324544BRJ
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP324544BRJ
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP324544BRJ-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP324544BRJ
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP324544BRJ
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

kinA

Uniprot No.

P16497

Alternative Names

kinA; gsiC; scoD; spoIIF; spoIIJ; BSU13990; Sporulation kinase A; EC 2.7.13.3; Stage II sporulation protein F; Stage II sporulation protein J

Species

Bacillus subtilis (strain 168)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP.

Gene References into Functions

These data indicated that the autokinase activity is dependent on the formation of a functional tetramer, which is mediated by, at least, the PAS-B and PAS-C domains. PMID: 28449380
autophosphorylation of the major sporulation histidine kinase KinA is able to occur in trans within the homotetramer complex PMID: 26055117
Findings suggest that dimerization of PAS(B) is important for keeping the catalytic domain of KinA in a functional conformation. We use this information to propose a model for the structure of the N-terminal sensor module of KinA. PMID: 23504013
Tetramer formation mediated by the N-terminal domain of kinA is important for the kinase activity catalyzed by the C-terminal domain. PMID: 21926229
Sda slows the rate of phosphotransfer from KinA to its target, Spo0F. PMID: 19040634

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Database Links

KEGG: bsu:BSU13990

STRING: 224308.Bsubs1_010100007766

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