Human E-Selectin ELISA kit

Code CSB-E04540h
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
selectin E
Alternative Names
SELE; ELAM1; E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD antigen CD62E
Abbreviation
SELE
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Linearity

Recovery

Typical Data

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The human SELE ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure human SELE in serum, plasma, or homogenates. It is based on the Sandwich-ELISA mechanism. SELE in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated SELE antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the SELE bound in the initial step. The SELE concentration can be calculated according to the standard curve. This kit is tested with high sensitivity and strong specificity.

SELE is a glycoprotein expressed on inflamed vascular endothelial cells in response to the inflammatory cytokines TNF-α and IL-1β. It mediates leukocyte rolling and vascular adhesion through interaction with carbohydrate ligands and thus is involved in the recruitment of neutrophils, monocytes, and T cells to inflammatory foci. Many studies have shown that endothelial cell SELE plays an important role in mediating cell-cell interactions between tumor cells and endothelial monolayers during tumor metastasis. SELE-mediated adhesion of circulating tumor cells to vascular endothelial cells (CTCs) initiates CTC extravasation at a distant site and is hence a critical step of the metastatic cascade.

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Target Background

Function
(From Uniprot)
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Gene References into Functions
  1. Results highlight novel mechanisms by which p38 downregulates the expression of E-selectin through miR-146a and miR-31 following inflammatory stimuli associated to cancer progression. PMID: 29402939
  2. Levels of E and P selectin showed positive correlation with the length of stay. PMID: 29517629
  3. Our study shows a significant association of E-selectin gene polymorphism(Leu554Phe) with essential hypertension. PMID: 29178542
  4. The highest levels of sL- and sE-selectin were observed in colorectal patients with lymph node metastasis. PMID: 27775438
  5. Circulating CD62E(+) microparticle levels are abnormal in subjects with pre-diabetes. PMID: 28927403
  6. sVCAM-1 reflects xerostomia in primary Sjogren's syndrome. sICAM-1 and sE-selectin may be additional parameters of secondary Sjogren's syndrome activity. PMID: 29068581
  7. Neutrophil rolling over E-selectin at precise shear stress transmits tension and catch-bond formation with L-selectin via sLe(x), resulting in focal clusters that deliver a distinct signal to upshift beta2-integrins to a high-affinity state. Rivipansel effectively blocked formation of selectin catch-bonds, revealing a novel mechanotransduction circuit PMID: 28811304
  8. E-selectin-dependent rolling promotes adhesion to HA by both physically slowing cells and enabling them to position proximal to the surface, thereby increasing the on rate of adhesion. PMID: 28765175
  9. High E-selectin expression is associated with breast cancer metastasis. PMID: 27220365
  10. his study shows that two adhesion molecules, shed as soluble forms, are elevated during the acute phase of leptospirosis: E-selectin and s-ICAM1. These molecules may interfere with the process of immune cell recruitment to clear Leptospira at tissue levels. PMID: 28686648
  11. ELAM-1 upregulation may promote disorder in adhesion, and migration of the cells leading to the endometriosis disorder. PMID: 27433816
  12. Elevated endothelium-related mediators (vWF, E-selectin and EPCR) appear to participate in the development of pancreatic necrosis and may be a potential indicator of overall prognosis. PMID: 28007459
  13. interplay between flow-dependent on-rate and off-rate of E-selectin-ligand bonds determine flow-enhanced cell rolling stability PMID: 27558713
  14. High SELE expression is associated with Inflamed Atherosclerotic Plaque. PMID: 27237221
  15. In this study, we addressed this question by analysing sLex expression together with two glycoproteins (BST-2 and LGALS3BP), shown to interact with E-selectin in a carbohydrate-dependent manner, in a cohort of 249 invasive breast cancers PMID: 27176937
  16. SELE expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  17. E-selectin concentrations in this study were associated with hyperglycemia, possibly reflecting early endothelial damage. E-selectin was not useful to assess carotid intima-media thickness, a marker of subclinical atherosclerosis, which appeared to be determined by aging and male gender. PMID: 27141013
  18. Plasma E-selectin is overexpressed in pediatric patients with mycoplasma pneumoniae pneumonia and myocardial injury. PMID: 26843511
  19. The significant presence of CLA+ T cells and E-selectin (CD62E) expressions in the OLPG suggests their involvement in the etiopathogenesis of oral lichen planus ; however, only a weak correlation between CLA+ T cells and E-selectin was observed. PMID: 26332489
  20. BD patients have a lower expression of ICAM-1, VCAM-1, and E-selectin in their tumor tissues. BD-HDL facilitates the adhesion of tumor cells to vascular endothelium by upregulating the expression of ICAM-1 and VCAM-1, thereby promoting the initial progression of breast cancer metastasis PMID: 26872904
  21. The E-selectin S149R polymorphisms is associated with the oncogenesis of breast cancer. PMID: 26828984
  22. Neisseria meningitidis caused a high level of E-selectin expression elicited by the activity of phosphorylated ATF2 transcription factor on the E-selectin promoter. PMID: 26153406
  23. E-selectin is more effective than cTnI in diagnosing myocardial injury in children. PMID: 26617843
  24. Inhibition of E-selectin expression allows an anti-tumoral effect on sLex-expressing hepatocellular carcinoma tumors in vivo PMID: 26988221
  25. Glycosphingolipids likely contribute to human myeloid cell adhesion to E-selectin under fluid shear, particularly the transition of rolling cells to firm arrest. PMID: 26868209
  26. Data show that both mucin16 (MUC16) and podocalyxin (PODXL)-E-selectin-mediated interactions are mechanically stronger than like L-selectin interactions at the single-molecule level. PMID: 26329844
  27. These findings provide consistent evidence that A516C and G98T polymorphisms of the SELE gene may be associated with increased susceptibility of coronary artery disease. PMID: 26937892
  28. Glaucomatous MYOC mutations activate the IL-1beta/NF-kappaB inflammatory stress response and the glaucoma marker SELE in trabecular meshwork cells. PMID: 26396484
  29. P. gingivalis was shown to activate NOD1, NOD2, and TLR2 expression, resulting in increased E-selectin expression in endothelial cells; direct inhibition of NF-kappaB and P38 MAPK significantly attenuated E-selectin expression induced by P. gingivalis in endothelial cells PMID: 25939768
  30. The number of E-selectin-positive cells was significantly higher in the tuberculoid form than in the lepromatous form of leprosy skin lesions. PMID: 26051909
  31. Gene-based study revealed significant associations of the SELE gene with Blood Pressure phenotypes for the first time among the Chinese Han population. PMID: 25424718
  32. This study showed that no significant differences in F206L Polymorphism of l-Selectin Between the Patients with Bronchial Asthma and Control. PMID: 24798295
  33. Blood ESEL was higher in pregnant women with preeclampsia, and non-pregnant women with a history of preeclampsia than women without preeclampsia or a history of preeclampsia. PMID: 26116870
  34. The findings suggest that C allele carriers of the SELE gene polymorphism (A561C) might be predisposed to EH in the Chinese population. PMID: 25171839
  35. E-selectin promoter was highly and specifically acetylated in the confluent monolayer after tumor necrosis factor alpha activation. PMID: 24690766
  36. E-selectin interactions with glycoprotein ligands (CD44/hematopoietic cell E-/L-selectin ligand and PSGL-1) mediate the initial capturing of cells out of flow. PMID: 26124272
  37. SELE rs3917412 and MTHFR rs1801133 SNPs could serve as pharmacogenetic predictors of tumor recurrence in patients with early-stage colon cancer treated with oxaliplatin-based adjuvant chemotherapy PMID: 24980946
  38. sE-selectin and the LDL were the most important predictors of late arteriovenous fistula stenosis PMID: 24627030
  39. The C allele of E-selectin A561C gene polymorphism might increase the essential hypertension (EH) risk in Asian population, whereas the T allele of E-selectin C1839T gene polymorphism might decrease the EH risk. [meta-analysis] PMID: 25003340
  40. Bereaved respondents had higher levels of inflammatory biomarkers IL-6 and sE-selectin, but not CRP and sICAM-I than the non-bereaved. PMID: 24266503
  41. SE-selectin and PAPPA may contribute to future cardiovascular events in women post preeclampsia; in contrast, ApoB may be involved in a protective mechanism PMID: 25238218
  42. the haplotype TCC in the Han hypertensive patients and the haplotype GGC in the Mongolian patients had independent effects in increasing the risk for essential hypertension; multiple single-nucleotide polymorphisms in combination in SELE may confer a risk of hypertension. PMID: 25748737
  43. Found the AC genotype to be a significant risk factor for hemorrhagic stroke and we also found significant differences in AC genotype and C allele among hemorrhagic stroke and ischemic stroke patients. PMID: 25168603
  44. The rs5361 E-selectin gene polymorphism is not a susceptibility marker for ACS in Western Mexico population. However, sE-selectin may be a biological marker of ACS. PMID: 25147432
  45. ST3Gal-4 is the primary sialyltransferase regulating the synthesis of E-, P-, and L-selectin ligands on human myeloid leukocytes PMID: 25498912
  46. an upregulation of E-selectin and the associated influx of neutrophils may play a significant role in the resolution of inflammation as well as for the pathophysiology of nasal polyps of Caucasian chronic rhinosinusitis patients. PMID: 24995349
  47. in human aortic and vein endothelial cells, expression and release of ICAM-1 and E-selectin were induced by treatment with conditioned medium collected from Pal-stimulated pTHP-1 cells PMID: 25201247
  48. CD62E was found to be a new biomarker for patients with hypertension and hyperlipidemia. PMID: 24820735
  49. High E-Selectin expression is associated with small cell lung cancer metastasis. PMID: 24699516
  50. there was association between the E-selectin S128R polymorphism and the risk of cancer. PMID: 24815478

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Selectin/LECAM family
Database Links

HGNC: 10718

OMIM: 131210

KEGG: hsa:6401

STRING: 9606.ENSP00000331736

UniGene: Hs.82848

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