Recombinant Mouse Polycomb protein EED (Eed)

1. Eed, an essential subunit of PRC2, is crucial to maintain the identity of CD4(+) T cells under TGFbeta-induced regulatory T cell (Treg)-polarizing conditions. PMID: 29310022
2. Eed deficiency in chondrocytes causes severe kyphosis and a growth defect with decreased chondrocyte proliferation, accelerated hypertrophic differentiation and cell death with reduced Hif1a expression PMID: 27329220
3. The progenitors in Eed mutant mice that were induced to differentiate did not develop into properly formed nephrons. Lhx1, normally expressed in the renal vesicle, was overexpressed in kidneys of Eed mutant mice. Thus, PRC2 has a crucial role in suppressing the expression of genes that maintain the progenitor state, allowing nephron differentiation to proceed. PMID: 29945864
4. discovery of differentiation stage-specific and dose-dependent roles of EED in normal hematopoiesis and leukemogenesis PMID: 27432459
5. our work provides in vivo evidence that the structural integrity of EED to H3K27me3 propagation is critical, especially for embryonic development and hematopoietic homeostasis, and that its perturbation increases the predisposition to hematologic malignancies PMID: 27578866
6. Deletion of Eed but not Ezh2 from embryonic urothelial progenitors caused premature differentiation of Krt5+ basal cells and ectopic expression of squamous cell markers. PMID: 28049658
7. in the skin epithelium, EED, Suz12, and Ezh1/2 function largely as subunits of the PRC2 complex and have roles in skin development PMID: 26994968
8. EED is required for proper erythropoiesis and for formation of hematopoietic progenitor and stem cells, but is dispensable for endothelial lineage commitment and early vascular patterning PMID: 28406475
9. Genetic inactivation of Ezh2 or Eed cooperates with NRASQ61K in leukemogenesis. PMID: 26904942
10. EED affects the lymphoid versus myeloid decision processes within the lymphomyeloid lineage PMID: 25961873
11. Inactivation of Eed impedes MLL-AF9-mediated leukemogenesis through Cdkn2a-dependent and Cdkn2a-independent mechanisms in a murine model. PMID: 26118502
12. Data suggest that Eed (embryonic ectoderm development) is necessary to silence the pluripotency network during differentiation. PMID: 25134795
13. An integral role for EED as an epigenetic exchange factor coordinating the activities of PRC1 and 2, is reported. PMID: 24457600
14. a microRNA encoded by the imprinted Dlk1-Dio3 region of mouse chromosome 12, miR-323-3p, targets Eed (embryonic ectoderm development) mRNA. PMID: 23821546
15. Ectopic gain of EED along with depletion of KDM6B in preimplantation mouse embryos abrogates CDX2 and GATA3 gene expression in the nascent trophoectoderm lineage. PMID: 23671187
16. It was shown that Ring1B interacted with multiple complexes in embryonic stem cells. Although H2A.Z co-localized with Eed, Ring1B and CpG islands in chromatin, H2A.Z still blanketed polycomb target loci in the absence of Suz12, Eed, or Ring1B. PMID: 22496869
17. Data indicate that binding of EZH2, SUZ12 and EED, the 3 subunits of Plybomb repressive complex 2 (PRC2), is required for PRC2 full activity, and late during viral infection, a significant increase in PRC2 protein association with chromatin. PMID: 22279536
18. The role of polycomb repressive complex 2 (PRC2) function in leukemia development and progression, is reported. PMID: 22396593
19. identify polycomb-like 2 and Polycomb Repressive Complex 2 transcriptional targets and draft regulatory networks.[Polycomb Repressive Complex 2 and polycomb-like 2] PMID: 20144788
20. Embryonic cells homozygous for a null mutation were shown to respond properly to gastrulation signals, but failed to differentiate into forebrain and also segregated themselves from normal cells in the somites. PMID: 11783004
21. Eed and a second Polycomb group protein, Enx1, are directly localized to the inactive X chromosome in XX trophoblast stem (TS) cells PMID: 12123576
22. The anterior expression boundaries of several Hox genes were shifted rostrally by one segment, indicating that Eed is required for stable repression of homeotic genes. PMID: 12370779
23. Eed is a member of a new class of trans-acting factors that regulate parent-of-origin expression at imprinted loci PMID: 12627233
24. Eed has a role in genetic imprinting in mice [review] PMID: 12665859
25. Eed is required for global histone H3 lysine-27 methylation. PMID: 15916951
26. Results demonstrate that the inactive X-chromosome in Eed(-/-) trophoblast stem cells and in cells of the trophectoderm-derived extra-embryonic ectoderm in Eed(-/-) embryos remain transcriptionally silent. PMID: 16415857
27. EED is dispensable for the initiation of random X-chromosome inactivation PMID: 16680199
28. Eed associates with the trxG protein Mll upon brain maturation. PMID: 17259173
29. These results suggest a common mechanism for random monoallelic expression on autosomes and the X chromosome, and implicate Eed in establishing differences between homologous olfactory receptor loci before and after differentiation. PMID: 17954609
30. core WD-40 motifs and the histone-binding region of EED alone are sufficient for the generation of all three marks, demonstrating that EED isoforms do not control the number of methyl groups added to H3K27 PMID: 17997413
31. STAT3 and Oct-3/4 maintain silencing of differentiation-associated genes through up-regulation of Eed in self-renewing ES cells. PMID: 18201968
32. Deficiency for the Polycomb group protein EED, leads to biallelic expression of Tfpi2 in the extra-embryonic lineages, whereas the other genes in the cluster maintain correct imprinting. PMID: 18480470
33. Male embryonic stem cells with Eed-null and Tsix mutations (X(Delta)Y Eed-/-) showed Xist hyperactivation upon differentiation, whereas cells with either mutation alone did not. PMID: 18511907
34. Eed are significantly downregulated during murine skin repair. PMID: 19575012

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KEGG: mmu:13626

STRING: 10090.ENSMUSP00000102853

UniGene: Mm.380914