Recombinant Listeria innocua serovar 6a D-alanine aminotransferase (dat)

Code CSB-YP842194LGU
MSDS
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Source Yeast
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Code CSB-EP842194LGU
MSDS
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Source E.coli
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Code CSB-EP842194LGU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842194LGU
MSDS
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Source Baculovirus
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Code CSB-MP842194LGU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dat
Uniprot No.
Alternative Names
dat; daaA; lin1660D-alanine aminotransferase; EC 2.6.1.21; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase
Species
Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)
Expression Region
1-289
Target Protein Sequence
MKVLVNDRLV ERNDATVDVE DRGYQFGDGV YEVVRLYNGK FFTYNEHIDR LYASAAKIDL VIPYSKETLR ALLDKLVAEN NINTGNVYLQ VTRGVQNPRN HVLPDDFPLE GVLTAAAREV PRNERQFIEG GSAITEEDVR WLRCDIKSLN LLGNILAKNK AHQQNALEAI LHRGEQVTEC SASNVSIIKD GVLWTHAADN LILNGITRQV IIDVAKKNGI PVKEADFTLT DLREADEVFI SSTTIEITPI THIDGVQVAD GKRGPITAQL HNYFVEEIVR ACGELEYAK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha-keto acid in the first half-reaction. The second half-reaction is the reverse of the first, transferring the amino group from the pyridoxamine to a second alpha-keto acid to form the product D-amino acid via a ping-pong mechanism. This is an important process in the formation of D-alanine and D-glutamate, which are essential bacterial cell wall components.
Protein Families
Class-IV pyridoxal-phosphate-dependent aminotransferase family
Database Links

KEGG: lin:daaA

STRING: 272626.lin1660

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