fur Antibody

Code CSB-PA364302ZA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) fur Polyclonal antibody
Uniprot No.
Target Names
fur
Alternative Names
fur antibody; b0683 antibody; JW0669Ferric uptake regulation protein antibody; Ferric uptake regulator antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli
Immunogen
Recombinant Escherichia coli fur protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Acts as a global negative controlling element, employing Fe(2+) as a cofactor to bind the operator of the repressed genes. Regulates the expression of several outer-membrane proteins including the iron transport operon.
Gene References into Functions
  1. The influence of oxygen availability on the levels of active Fur adds a previously undescribed layer of regulation in maintaining cellular iron homeostasis in E. coli. PMID: 29087312
  2. High iron supply inhibits the synthesis of the genotoxin colibactin by pathogenic Escherichia coli through a non-canonical Fur/RyhB-mediated pathway. PMID: 28637194
  3. Finally, Fur derepression and RecA activation in enterohemorrhagic Escherichia coli O157 were shown to participate in the nitric oxide-enhanced Stx1 and Stx2 production, respectively. PMID: 28294553
  4. The authors report that O2 availability affects the gene expression programs of two Escherichia coli master regulators that function in iron homeostasis: the transcription factor Fur and the small RNA regulator RyhB. PMID: 26670385
  5. The key regulator of the response to serum-active and inactive-is the iron regulator Fur, which controls the expression of more than 80% of the serum-upregulated genes. PMID: 25118243
  6. Fur exhibits a comprehensive regulatory role affecting many fundamental cellular processes linked to iron metabolism in order to coordinate the overall response of E. coli to iron availability. PMID: 25222563
  7. Authors demonstrated that the EHEC lpf2 operon is regulated in response to temperature, pH, bile salts and iron, during the exponential phase of growth, and is controlled by Fur. PMID: 24966050
  8. In a murine model of urinary tract infection (UTI), deletion of fur alone did not attenuate virulence, whereas a DeltaryhB mutant and a Deltafur DeltaryhB double mutant showed significantly reduced bladder colonization. PMID: 25245805
  9. There is a transient change in the activity of the iron regulator Fur and of the oxidative stress regulator SoxS after Zn addition. PMID: 22016571
  10. results presented here show that ftnA iron induction is independent of RyhB and instead involves direct interaction of Fe(2+)-Fur with an 'extended' Fur binding site (containing five tandem Fur boxes) located upstream (-83) of the ftnA promoter. PMID: 20015147
  11. H2O2-inducible antioxidant defense system leading to an increase in catalase; hydroperoxidase HPI activity, is unaltered in Deltafur mutants PMID: 15878120
  12. Tyr 55 of Escherichia coli Fur, as well as the two thymines in positions 18 and 19 of the consensus Fur Box, are involved with binding. PMID: 16212958
  13. Affinity of Fur for different metals was determined & ranges over several orders of magnitude in the order: Zn(II)>> Co(II)> Fe(II)> Mn(II). Only Fe(II) binds with sufficient affinity to activate Fur significantly at physiological metal levels. PMID: 16216078
  14. an activation mechanism for E. coli Fur involves the folding/unfolding of the N-terminal helix PMID: 16690618
  15. Fur protein translation is coupled to that of an upstream open reading frame, translation of which is downregulated by the non-coding RNA RyhB. PMID: 17268550
  16. Fur function may be antagonized by intracellular H2O2 by oxidizing the Fur:Fe(2+) complex and inactivating repressor function. PMID: 17462026
  17. In this study, information theory was used to identify new Fur binding sites, 13 of which we confirmed experimentally. PMID: 17921503
  18. In the fur+ strain compared to those in the fur- strain, expression of chromosomal dihydroorotase activity and beta-galactosidase activity of pyrC-lacZ fusion plasmid was repressed to about 30% and 17%, respectively. PMID: 18176545
  19. The characteristic metabolism of rpoS mutants required the activity of Fur (ferric uptake regulator) in order to decrease the synthesis of the small RNA RyhB that might otherwise inhibit the synthesis of iron-rich proteins. PMID: 18556786
  20. Binding of both zinc and iron ions induced a similar change in Fur conformations, but shifted residues closer to DNA in a different manner. PMID: 19023664
  21. transcriptomic data identified the global transcription regulators ArcA, CRP, Fis, FNR, Fur, BaeR, CpxR, and IHF as targets and potential CO sensors PMID: 19091747

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Subcellular Location
Cytoplasm.
Protein Families
Fur family
Database Links
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