Mouse Leukemia inhibitory factor,LIF ELISA kit

Instructions
Code CSB-E04652m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leukemia inhibitory factor (cholinergic differentiation factor)
Alternative Names LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Differentiation-stimulating factor ELISA Kit; D factor ELISA Kit
Abbreviation LIF
Uniprot No. P09056
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %86-94
1:2Average %96
Range %90-100
1:4Average %92
Range %88-96
1:8Average %97
Range %92-101
Recovery
The recovery of mouse LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8480-88
EDTA plasma (n=4)9692-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.778 2.687 2.733 2.571
5002.209 2.036 2.123 1.961
2501.377 1.421 1.399 1.237
1250.671 0.725 0.698 0.536
62.50.343 0.317 0.330 0.168
31.250.265 0.274 0.270 0.108
15.60.224 0.237 0.231 0.069
00.165 0.159 0.162
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Gene References into Functions
  1. Inhibition of Lif protein in the hypothalamus transforms obesity-resistant mice into obesity-prone mice. PMID: 28865476
  2. mouse embryonic stem cells inside microchambers formed colonies and expressed markers of pluripotency in the absence of feeders or pluripotency-inducing signals such as leukemia inhibitory factor. PMID: 26865369
  3. lung epithelium, specifically alveolar type II cells, is the predominant site of LIF transcript induction in pneumonic mouse lungs. PMID: 28522567
  4. LIF/STAT3 and MEK/ERK signaling in naive embryonic stem cell pluripotency, is reported. PMID: 27676194
  5. LIF stimulates, in part, stem cell-derived cardiomyocyte regeneration by activating cardiac stem or precursor cells. PMID: 27227407
  6. Our data show that absence of SOCS2 turns cardiomyocytes unresponsive to LIF-induced [Ca(2+)] raise, indicating that endogenous levels of SOCS2 are crucial for full activation of LIF signaling in the heart. PMID: 28122728
  7. These results establish that Bcl3 positively regulates pluripotency genes and thus shed light on the mechanism of Bcl3 as a downstream molecule of LIF/STAT3 signaling in pluripotency maintenance. PMID: 26303070
  8. The role of LIF in acetylcholine synthesis via the STAT3, PI3 kinases, or cAMP-protein kinase A pathways, are reported. PMID: 25887270
  9. Either LIF or EGF is needed during development of pre-implantation embryo. PMID: 27096934
  10. Our data suggest that LIF plays an important role in placentation in vivo and the maintenance of healthy pregnancy. PMID: 26272398
  11. Despite common signal transduction mechanisms (JAK/STAT, MAPK and PI3K) LIF can have paradoxically opposite effects in different cell types including stimulating or inhibiting each of cell proliferation, differentiation and survival. PMID: 26187859
  12. Mechanistically, LIF activates STAT, which binds to a Stat consensus sequence in the Six2 proximal promoter and sustains SIX2 levels. PMID: 26321142
  13. These findings suggest that maternal IL-6 interferes the maternal-fetal LIF signal relay by inducing SOCS3 in the placenta and leads to decreased neurogenesis. PMID: 26043040
  14. Studied the expression of heme oxygenase-1 (HO-1) and leukemia inhibitory factor (LIF) in maternal plasma and placental tissue in intrauterine infection-induced preterm birth. PMID: 26995887
  15. LIF gene breakage was captured at the 18th hour after carbon disulfide exposure by Comet-FISH and the protein and mRNA of LIF in uterine tissue were down-regulated after carbon disulfide exposure through the peri-implantation period PMID: 26427868
  16. LIF has a role in primitive endoderm expansion during pre-implantation development PMID: 26395492
  17. Study shows that LIF-treated postmitotic oligodendrocytes secrete a number of neuropeptides at the mRNA and peptide level, and confirm LIF-induced galanin synthesis and secretion in primary rat oligodendrocytes but not in astrocytes PMID: 25639936
  18. In a preclinical animal model of MS shifting the LIF/IL-6 balance in favor of LIF by CNS-targeted overexpression increased the number of Tregs in the CNS during active autoimmune responses and reduced disease symptoms. PMID: 25514345
  19. LIF and miR-302 family positively affect the expression of pluripotency markers. PMID: 25147827
  20. these results reveal a novel function of miR-181 in embryo implantation through the regulation of LIF, and also indicate a potential link between miR-181 dysregulation and human embryo implantation defects. PMID: 25635127
  21. Expression of LIF protects the lung from lung injury and enhanced pathology during respiratory syncytial virus infection. PMID: 25277705
  22. Data suggest LIF induces dynamic/complex network of changes in endometrium, changes that appear essential to reproduction; changes involve gene expression regulation for cytoskeletal proteins and proteins involved in chromatin assembly/disassembly. PMID: 25031358
  23. The difference in the balance between the JAK-Stat3 pathway and the MAP kinase pathway underlies the differential response to LIF amongst mice strains. PMID: 25564647
  24. Data show that the contribution of leukemia inhibitory factor (LIF) for embryo implantation was dependent on the strains of mice. PMID: 24698551
  25. an essential function of Foxm1 in the LIF/Stat3-mediated mESC self-renewal and the generation of iPSCs PMID: 24743237
  26. LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway. PMID: 24464633
  27. Results show that endogenous levels of Pkig reciprocally regulate osteoblast and adipocyte differentiation and that this reciprocal regulation is mediated in part by LIF. PMID: 23963683
  28. study presents for the first time that estrogen regulates Egr1 expression through LIF-STAT3 signaling pathway in mouse uterus, and Egr1 functions as a critical mediator of stromal cell decidualization by regulating Wnt4 PMID: 25012664
  29. Addition of leukemia inhibitory factor (LIF) neutralizing antibodies inhibited oligodendrocyte differentiation, indicating a crucial role of TNFR2-induced astrocyte derived LIF for oligodendrocyte maturation PMID: 24310780
  30. Uterine kisspeptin signaling regulates glandular Lif levels, regulating embryo implantation PMID: 24877624
  31. This mini review will summarize the findings that are related to LIF signaling and discuss the neuroprotective effects of LIF in different animal models. PMID: 24664722
  32. Induced pluripotent stem cells-derived neural precursors exert a neuroprotective role in immune-mediated demyelination via the secretion of LIF. PMID: 24169527
  33. Expression of Lif in the injured retina and activation of the endogenous survival pathway involve signaling through p38 MAPK. PMID: 24008729
  34. Tfcp2l1 is the principal bridge between LIF/Stat3 input and the transcription factor core of naive pluripotency. PMID: 23942233
  35. A pivotal role of PPARgamma on mouse embryonic stem cell self-renewal depends on the presence and absence of LIF. PMID: 23628590
  36. LIF was able to modulate the levels of nitric oxide induced by LPS in vitro, suggesting that it could be a potential mediator of the inflammatory action of progesterone PMID: 23409146
  37. These studies demonstrate that endogenous CNTF and LIF act centrally to protect axons from acute inflammatory destruction via an oligodendrocyte-independent mechanism. PMID: 23077604
  38. we show that LIF increases the expression of the core transcription factors PMID: 23258129
  39. The rapid release of leukemia inhibitory factor from cortical neurons protects them against excitotoxicity. PMID: 22894638
  40. Acyloxy nitroso compounds inhibit LIF signaling in endothelial cells and cardiac myocytes, showing that STAT3 signaling is redox-sensitive PMID: 22905257
  41. These results provide further evidence for the role of LIF in inflammation and cartilage bone resorption and provide impetus to test the effects of LIF blockade as a therapeutic strategy in rheumatoid arthritis. PMID: 22774981
  42. The LIF-STAT3 axis is identified in this study as a critical determinant of lung injury with clinical implications for pneumonia patients. PMID: 22581855
  43. LIF controls vascular size and osteoclast differentiation during the transition of cartilage to bone, whereas an anatomically separate LIF-dependent pathway regulates osteoblast and adipocyte commitment in bone remodeling PMID: 22143976
  44. Aute short-term depletion of tet1 results in impairment of LIF-dependent Stat3-mediated gene activation. PMID: 22210859
  45. LIF expression might be regulated by microRNA-199a PMID: 22285730
  46. Liver sinusoidal endothelial cells constitutively express LIF at the mRNA and protein level. PMID: 21930391
  47. Study demonstrated that LIF was an important regulator of decidualization in humans and mice. PMID: 21966484
  48. Extensive mannose phosphorylation on leukemia inhibitory factor (LIF) controls its extracellular levels by multiple mechanisms. PMID: 21613225
  49. Blockade of LIF signalling results in a 50% decrease in embryo implantation. PMID: 19635738
  50. LIF removal results in the rapid phosphorylation of multiple proteins regulating murine embryonic stem cell self-renewal on Tyr, Ser, & Thr residues. These posttranslational modifications help drive the exit of mESCs from the pluripotent state. PMID: 22074622

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Subcellular Location Secreted
Protein Families LIF/OSM family
Database Links

KEGG: mmu:16878

STRING: 10090.ENSMUSP00000067066

UniGene: Mm.4964

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