Recombinant Borrelia burgdorferi Flagellar filament 41 kDa core protein (fla)

Amino acids 1-336 constitute the expression domain of recombinant Borrelia burgdorferi fla. This fla protein is theoretically predicted to have a molecular weight of 51.8 kDa. This fla protein is produced using e.coli expression system. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of fla, which enables a simple process of detecting and purifying the fla recombinant protein in the following steps.

The Borrelia burgdorferi Flagellar Filament 41 kDa Core Protein (Fla) is a structural component of the bacterial flagellum, a whip-like appendage that plays a crucial role in motility and chemotaxis. Borrelia burgdorferi is a spirochete bacterium responsible for causing Lyme disease, and its flagellum is essential for its movement in various environments. The Fla protein forms the core structure of the flagellar filament and is involved in the rotation of the flagellum, allowing the bacterium to navigate and migrate through different host tissues. Research on the Fla protein in Borrelia burgdorferi is crucial for understanding the bacterium's motility, which is essential for its transmission between hosts and the establishment of infection. Investigating the structure and function of Fla may contribute to the development of strategies to interfere with the bacterium's motility, potentially hindering its ability to cause Lyme disease.