Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
fla; BB_0147Flagellar filament 41 kDa core protein; Flagellin; 41 kDa antigen; P41
Species
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Expression Region
1-336aa
Target Protein Sequence
MIINHNTSAINASRNNGINAANLSKTQEKLSSGYRINRASDDAAGMGVSGKINAQIRGLSQASRNTSKAINFIQTTEGNLNEVEKVLVRMKELAVQSGNGTYSDADRGSIQIEIEQLTDEINRIADQAQYNQMHMLSNKSASQNVRTAEELGMQPAKINTPASLSGSQASWTLRVHVGANQDEAIAVNIYAANVANLFSGEGAQTAQAAPVQEGVQQEGAQQPAPATAPSQGGVNSPVNVTTTVDANTSLAKIENAIRMISDQRANLGAFQNRLESIKDSTEYAIENLKASYAQIKDATMTDEVVAATTNSILTQSAMAMIAQANQVPQYVLSLLR
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-336 constitute the expression domain of recombinant Borrelia burgdorferi fla. This fla protein is theoretically predicted to have a molecular weight of 51.8 kDa. This fla protein is produced using e.coli expression system. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of fla, which enables a simple process of detecting and purifying the fla recombinant protein in the following steps.
The Borrelia burgdorferi Flagellar Filament 41 kDa Core Protein (Fla) is a structural component of the bacterial flagellum, a whip-like appendage that plays a crucial role in motility and chemotaxis. Borrelia burgdorferi is a spirochete bacterium responsible for causing Lyme disease, and its flagellum is essential for its movement in various environments. The Fla protein forms the core structure of the flagellar filament and is involved in the rotation of the flagellum, allowing the bacterium to navigate and migrate through different host tissues. Research on the Fla protein in Borrelia burgdorferi is crucial for understanding the bacterium's motility, which is essential for its transmission between hosts and the establishment of infection. Investigating the structure and function of Fla may contribute to the development of strategies to interfere with the bacterium's motility, potentially hindering its ability to cause Lyme disease.