Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

OGN

Uniprot No.

P20774

Research Area

Signal Transduction

Alternative Names

Corneal keratan sulfate proteoglycan; DKFZP586P2421; MIME_HUMAN; Mimecan; Mimecan proteoglycan; OG; OGN; OIF; Osteoglycin; Osteoinductive factor; SLRR3A

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

21-298aa

Target Protein Sequence

PPTQQDSRIIYDYGTDNFEESIFSQDYEDKYLDGKNIKEKETVIIPNEKSLQLQKDEAITPLPPKKENDEMPTCLLCVCLSGSVYCEEVDIDAVPPLPKESAYLYARFNKIKKLTAKDFADIPNLRRLDFTGNLIEDIEDGTFSKLSLLEELSLAENQLLKLPVLPPKLTLFNAKYNKIKSRGIKANAFKKLNNLTFLYLDHNALESVPLNLPESLRVIHLQFNNIASITDDTFCKANDTSYIRDRIEEIRLEGNPIVLGKHPNSFICLKRLPIGSYF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

33.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To achieve the expression of the recombinant Human OGN protein in Yeast cells, a DNA fragment encoding the Human OGN protein (21-298aa) is inserted into a plasmid vector, which is then transferred to Yeast cells. After screening positive cells, they are cultured and induced to generate the OGN protein. A N-terminal 6xHis tag is attached to the protein. Cells are lysed to collect the recombinant Human OGN protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the obtained recombinant Human OGN protein is over 90%.

Target Background

Function

Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.

Gene References into Functions

OGN expression is positively associated with CD8+ cell density in colorectal cancer tissue, suggesting a possible influence of OGN expression on tumor reactive T cells in the tumor niche. PMID: 30037719
Results provide evidence that osteoglycin expression is increased in the heart in response to pressure overload and its absence results in increased cardiac inflammation and fibrosis resulting in increased diastolic dysfunction. PMID: 28958774
OGN identified as a novel oncogene in meningioma proliferation. PMID: 28931407
serum osteoglycin is a potential predictor of adverse outcomes in patients with chronic kidney disease. PMID: 28824047
NK cells promote fetal development through the secretion of growth-promoting factors, pleiotrophin and osteoglycin. PMID: 29262349
Increased serum mimecan is associated with poor angiographic coronary collateralization in patients with chronic total occlusion PMID: 27508318
the association of serum OGN and FAM5C levels and muscle mass with bone mineral density (BMD), bone turnover markers, and the presence of vertebral fractures (VFs) in 156 postmenopausal women with type 2 diabetes mellitus, is reported. PMID: 27836731
OGN plays a critical role in negatively regulating ischaemia-induced angiogenesis by inhibiting VEGF-VEGFR2 signalling and thereby attenuating endothelial cells tube formation, proliferation, and migration. PMID: 28069703
The current study discovered a novel 72-kDa chondroitin sulfate-OGN that is specific for innate immune cells. This variant is able to bind and activate TLR4. PMID: 27878326
mimecan is a satiety hormone in adipose tissue, and that mimecan inhibits food intake independently of leptin signaling by inducing IL-1beta and IL-6 expression in the hypothalamus. PMID: 26870797
Increased plasma mimecan levels are independently associated with increased arterial stiffness in hypertensive patients. PMID: 26206738
OIF may be an indicator of the earlier-stage diabetic nephropathy in subjects with type 2 diabetes mellitus PMID: 26045825
OGN might play a role in the development of Ovarian Cancer, and may be a therapeutic target in OC. PMID: 25953442
The lack of the proteoglycan OGN does not affect the progression of atherosclerosis in mice. Possible causes for the absence of phenotype in the Apoe/Ogn double mutants are discussed. PMID: 25463067
Circulating OGN and NGAL/MMP9 complex are promising biomarkers that are expressed in vulnerable atherosclerotic plaques and may have incremental value for prediction of MACE within 1 year after coronary angiography. PMID: 24651681
We found that IGF-2 and IGFBP2 synergistically increased neurite outgrowth via enhanced early signaling through the IGF type 1 receptor. PMID: 23714241
This work provides insight into the regulating mechanism of mimecan in pituitary and suggests that mimecan may be an unidentified pituitary secretory protein, and certain pituitary cells secreting ACTH or GH also secrete mimecan. PMID: 16189248
All adenoma and cancer tissues did not express mimecan, but all normal mucosa did (P < 0.01). PMID: 17895523
High expression in the cochlea may be suggestive of a fundamental role for a transcript in the auditory system PMID: 18243607
Includes the characterization of two human splice variants. PMID: 10373482

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

Small leucine-rich proteoglycan (SLRP) family, SLRP class III subfamily

Tissue Specificity

Bone.

Database Links

HGNC: 8126

OMIM: 602383

KEGG: hsa:4969

STRING: 9606.ENSP00000262551

UniGene: Hs.109439

Code	CSB-YP016314HU
Abbreviation	Recombinant Human OGN protein
MSDS	MSDS Datasheet
Size	$250
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP016314HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) OGN. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP016314HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) OGN.
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Recombinant Human Mimecan (OGN)

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