Recombinant Human Leukemia inhibitory factor protein(LIF) (Active)

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Code CSB-AP002221HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.
Target Names LIF
Uniprot No. P15018
Research Area Immunology
Alternative Names CDF; Cholinergic Differentiation Factor ; D factor; DIA; Differentiation inducing factor; differentiation inhibitory activity; Differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin ; Hepatocyte stimulating factor III; HILDA; Human interleukin in DA cells; Leukemia inhibitory factor; LIF; LIF_HUMAN; Melanoma derived LPL inhibitor; Melanoma-derived LPL inhibitor; MLPLI
Species Homo sapiens (Human)
Source E.coli
Expression Region 23-202aa
Complete Sequence SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Mol. Weight 19.7 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Gene References into Functions
  1. miR-181c-3p and -5p promotes high-glucose-induced dysfunction in human umbilical vein endothelial cells by regulating leukemia inhibitory factor PMID: 29605252
  2. The findings indicate that low LIF concentrations in serum and follicular fluid may contribute to disordered folliculogenesis in polycystic ovary syndrome. PMID: 29397316
  3. This study uses LIF to activate the PI3K/ AKT signal and induce the anti-inflammatory effect during the neuron differentiation from human induced pluripotent stem cell-derived neural precursor cells. PMID: 29393372
  4. These findings implicate ZEB1 as a stem cell regulator in glioma via LIF repression which when deleted leads to increased stemness, tumorigenicity and shortened patient survival. PMID: 28246407
  5. Decreased serum LIF levels may be associated with vasculopathy in systemic sclerosis (SSc) and that Fli1 deficiency may contribute to the inhibition of LIF-dependent biological effects on SSc endothelial cells by suppressing the expression of LIF, LIF receptor, and gp130. PMID: 29038846
  6. The endometrial expression of LIF and CD34 in the pathogenesis of non-developing pregnancy can be used for evaluating the pregnancy prognosis in women of young and old reproductive age. PMID: 29063331
  7. This report describes the involvement of proteins responsible for cell growth and progression and defines the LIF-mediated novel autocrine-paracrine signaling loop for cell growth arrest. PMID: 28755912
  8. The expression of LIF was associated with tumor size and a poorer overall survival. Microarray and quantitative real-time polymerase chain reaction assessments suggest that LIF can facilitate tumor-promoting inflammation. Results indicate that LIF plays a role in maintaining cancer stem cells in chordomas. PMID: 28247842
  9. study indicated impaired LIF expression levels only in women with unexplained infertility, while LIF-R expression was impaired in all sub-groups of infertile women. PMID: 28432985
  10. findings illustrate that DeltaNp63alpha can inhibit the levels of LIF mRNA by direct transcription regulation and decrease LIF mRNA stability by suppressing the expression of Lnc-LIF-AS. An inverse interaction of LIF and DeltaNp63alpha expression was as well validated in clinical samples of cervical cancer, and high level of LIF in cervical cancers was related with poor patient survival. PMID: 28391028
  11. ATF3 plays a significant role in regulating human endometrial receptivity and embryo attachment in vitro via up-regulation of leukemia inhibitory factor. PMID: 28577574
  12. Although further studies would be required to deconvolute the targets involved in LIF induction and to confirm activity of hits in more disease-relevant assays, our results have demonstrated the potential of the phenotypic approach to identify specific and chemically tractable small molecules that trigger the production of LIF in relevant cell lines PMID: 26984928
  13. SNP 3951C/T of LIF may not be associated with in vitro fertilization and embryo transfer outcome in Iranian population. PMID: 28466814
  14. In summary, this study has shown that LIF is implicated in the HG-mediated inhibition of osteoblast differentiation, via promoting STAT3/SOCS3 signaling. This study may provide insights into the signal pathway of HG-induced bone loss or delayed injured joint healing. PMID: 28064096
  15. Cytokines of the LIF/CNTF family and metabolism PMID: 26817395
  16. These results demonstrate the involvement of PIM kinases in LIF-induced regulation in different trophoblastic cell lines which may indicate similar functions in primary cells. PMID: 28729093
  17. Data suggest a 216-nucleotide proximal cis-element in LIF mRNA exhibits mRNA destabilizing potential; on exposure to carcinogen PMA (phorbol-12-myristate-13-acetate), this cis-element exhibits mRNA stabilizing activity. PMA induces nucleo-cytoplasmic translocation of both nucleolin and PCBP1, 2 trans-acting factors that bind to and stabilize LIF mRNA. [LIF = leukemia inhibitory factor; PCBP1 = poly(rC) binding protein 1] PMID: 28512205
  18. Leukemia inhibitory factor (LIF) - STAT3 transcription factor (STAT3) signaling pathway is systemically dysregulated in in the endometrium of patients with recurrent/repeated implantation failure (RIFE). PMID: 27304912
  19. Endometrial expression of LIF and LIFR is significantly reduced in the epithelial cells of infertile women. PMID: 27082016
  20. LIF SNP T/G (rs929271) seems to be a susceptibility biomarker capable of predicting implantation efficiency and pregnancy outcomes. PMID: 26615902
  21. Overexpression of LIF promotes Epithelial-mesenchymal transition and results in cancer. PMID: 26716902
  22. This review discusses the role of LIF and the recent analysis of its action on the uterine LE in regulating endometrial receptivity and implantation. PMID: 26817565
  23. Women with dormant genital tuberculosis were found to have decreased endometrial LIF-STAT3 signaling. PMID: 26776907
  24. Data show that leukemia inhibitory factor (LIF) signaling promote chemoresistance in cholangiocarcinoma by up-regulating myeloid cell factor-1 (Mcl-1) via phosphatidylinositol 3-kinase (PI3K)/c-akt protein (AKT)-dependent pathway. PMID: 26296968
  25. Despite common signal transduction mechanisms (JAK/STAT, MAPK and PI3K) LIF can have paradoxically opposite effects in different cell types including stimulating or inhibiting each of cell proliferation, differentiation and survival. PMID: 26187859
  26. Taken together, we firstly demonstrate that LIF enhances the adhesion of trophoblastic cells to endometrial cells by up-regulating expression of integrin heterodimer alphaVbeta3 and alphaVbeta5. PMID: 26723254
  27. LIF has a role in negatively regulating tumour-suppressor p53 through Stat3/ID1/MDM2 in colorectal cancers PMID: 25323535
  28. LIF/p21 signaling cascade as a novel tumor suppressive-like pathway in melanoma, acting downstream of TGFbeta to regulate cell cycle arrest and cell death, further highlight new potential therapeutic strategies for the treatment of cutaneous melanoma PMID: 25885043
  29. LIF was frequently overexpressed in osteosarcoma, which could promote the growth and invasion through activating the STAT3 pathway. PMID: 26271643
  30. LIF structure, signaling pathway, and primary roles in the development and function of an organism are reviewed--{REVIEW} PMID: 25879318
  31. LIF downregulates the autoimmune response by enhancing Treg numbers. PMID: 25514345
  32. LIF mediates fibroblast activation to promote invasive tumor microenvironment. PMID: 24857661
  33. Data indicate that NanoLuc-fusion strategy provided an efficient approach for preparation of recombinant leukemia inhibitory factor (LIF) protein PMID: 25179300
  34. Expression of LIF protects the lung from lung injury and enhanced pathology during respiratory syncytial virus infection. PMID: 25277705
  35. Studied the association of tubal pregnancy with leukemia inhibitory factor (LIF) and leukemia inhibitory factor receptor (LIFR) expression in oviduct tissues. PMID: 25790555
  36. an essential function of Foxm1 in the LIF/Stat3-mediated mESC self-renewal and the generation of iPSCs PMID: 24743237
  37. Results show that endogenous levels of Pkig reciprocally regulate osteoblast and adipocyte differentiation and that this reciprocal regulation is mediated in part by LIF. PMID: 23963683
  38. An increased mRNA expression of PROK1 and LIF could be one of the several abnormalities characterizing the endometrium in women recurrent pregnancy loss. PMID: 25128195
  39. Addition of leukemia inhibitory factor (LIF) neutralizing antibodies inhibited oligodendrocyte differentiation, indicating a crucial role of TNFR2-induced astrocyte derived LIF for oligodendrocyte maturation PMID: 24310780
  40. Data from 3-year-old girl and other reported cases support model in which abundance of LIF in B-cell acute lymphoblastic leukemia results in leukemic infiltration of central nervous system and development of Cushing syndrome. [CASE STUDY; REVIEW] PMID: 23729555
  41. This mini review will summarize the findings that are related to LIF signaling and discuss the neuroprotective effects of LIF in different models. PMID: 24664722
  42. Data suggest LIF/LIF receptor (alpha subunit) signal transduction facilitates blastocyst implantation or development of tubal pregnancy by stimulating blastocyst adhesion and outgrowth/proliferation of placenta or Fallopian tube epithelial cells. PMID: 24074901
  43. Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT1. PMID: 23876532
  44. NPC patients had increased serum levels of LIF. Higher LIF levels correlated with local tumor recurrence. Xenograft mouse studies showed that LIF critically contributes to NPC tumor growth & radioresistance. PMID: 24270418
  45. study found lower expression of LIF in the edndometrium in unexplained infertile women with multiple implantation failures compared to fertile women; data suggest that the initial lower expression of LIF in proliferative phase may be one of the causes for multiple failure of implantation PMID: 23541977
  46. novel expression and purification protocol for the production of recombinant hLIF PMID: 23628981
  47. In summary, we can show that LIF is an important factor in melanoma progression. PMID: 23831429
  48. Data show that the expressions of ER-alpha, PR, LIF, VEGF, iNOS and CB1 in fallopian tube and chorionic villi of tubal pregnancy were not altered by exposure to levonorgestrel emergency contraception. PMID: 23687977
  49. LIF is a contraction-induced myokine, potentially acting in an autocrine or paracrine fashion to promote skeletal muscle satellite cell proliferation. PMID: 21527666
  50. No correlation was found between pinopodes development stage and LIF expressions in endometrium. PMID: 22252755
  51. LIF combined with VEGF can maintain the preliminary, progenitor phenotype of EPCs and alleviate cell differentiation by upregulating KLF4, which may provide new insights into transcriptional regulation in endothelial progenitor cells PMID: 22835519
  52. Identification of novel molecules associated with a LIF-mediated increase in trophoblastic cell invasion may facilitate our understanding of implantation biology. PMID: 23122949
  53. Data show that LIF through STAT3 negatively regulates Th2 differentiation. PMID: 22712053
  54. Acyloxy nitroso compounds inhibit LIF signaling in endothelial cells and cardiac myocytes, showing that STAT3 signaling is redox-sensitive PMID: 22905257
  55. LIF was expressed in endometrium of fertile women but significantly decreased in infertile women. Infertile women secreted significantly less LIF molecules in uterine flushing compared with fertile women. PMID: 22520363
  56. findings suggest that our LIF-dependent human iPSCs could provide a novel model to investigate the role of cytokine signaling in cellular reprogramming PMID: 22720020
  57. there is an altered expression of LIF and IL-15 in the endometrium of women with recurrent implantation failure PMID: 22537815
  58. plasma concentrations is a predictive marker of in vitro fertilization outcome PMID: 22436290
  59. Expression of LIF and integrin-beta3 (ITGB3) in the peri-implantation endometrium does not change in women with unexplained recurrent pregnancy loss. PMID: 22698642
  60. Although leukemia inhibitory factor (LIF) and LIF receptors were increased in muscle tissue and myoblasts from diabetic patients, LIF signaling and LIF-induced cell proliferation were impaired in diabetic myoblasts. PMID: 22649064
  61. LIF expression might be regulated by microRNA-199a PMID: 22285730
  62. LIF mRNA expression was significantly decreased in abnormal uterine cavities during the midsecretory phase, indicating that endometrial cavity defects are a possible cause of poor reproductive outcomes. PMID: 22341639
  63. Addition of IL-4 or IL-13 had no effect on IL-6 expression, but significantly inhibited LIF and IL-11 mRNA and protein stimulated by IL-1beta and TNF-alpha. PMID: 22142941
  64. LIF delivery can be harnessed to enhance the generation of oligodendrocytes that express myelin proteins and reform the nodes of Ranvier in the context of chronic demyelination in the adult mouse hippocampus. PMID: 22323722
  65. Study demonstrated that LIF was an important regulator of decidualization in humans and mice. PMID: 21966484
  66. Extensive mannose phosphorylation on leukemia inhibitory factor (LIF) controls its extracellular levels by multiple mechanisms. PMID: 21613225
  67. Endometrial HOXA11 and LIF mRNA expression levels (normalized to beta-actin expression) were significantly decreased in endometrium of infertile patients with endometriosis compared with healthy fertile controls PMID: 21987111
  68. stimulation with alpha(1)-adrenoceptor may enhance DNA synthesis and proliferation of mouse iPS cells cultured with LIF via augmentation of both the MEK/MAPK and the PI3K/Akt pathways PMID: 21745467
  69. MiR-141 has been significantly downregulated by more than 50% after leukemia inhibitory factor stimulation PMID: 21726338
  70. LIF could be significantly involved in the pathophysiology of Multiple sclerosis PMID: 21570299
  71. TGFbeta1, TNFalpha and LIF expressions showed increased level in the untreated patients with de novo acute myeloid leukemia. PMID: 21129244
  72. The epigenetic up-regulation of the LIF gene likely play an important role in the development of breast cancer. PMID: 21191816
  73. Serum concentrations of IL-6 family cytokines, including IL-6, IL-11, and LIF, were significantly elevated in patients with RA compared to those of healthy controls. PMID: 21155043
  74. Patients with recurrent spontaneous abortion not only displayed a decrease in LIF expression but also had a reduced frequency of endometrial infiltrated CD4+ LIF+ cells in comparison with fertile women. PMID: 20618183
  75. The cytokine LIF promotes trafficking of T-type calcium channel subunits in human HEK293 cells via transient activation of JAK- and ERK-kinase signaling pathways. PMID: 21178106
  76. Wharton's jelly - and adipose tissue-mesenchymal stromal cells (MSCc) were more potent than BMbone marrow-MSCs in suppressing lymphocyte responses, and they mediated this effect by secreting high levels of leukemia inhibitory factor. PMID: 20597819
  77. OPN, IL-6 and LIF may have critical roles in human pregnancy maintenance. PMID: 20851233
  78. The key features of leukaemia inhibitory factor cytokine, its numerous physiological effects the most recent data are presented. Review. PMID: 19541590
  79. Suggest that growth inhibition and activation of the autocrine/paracrine signaling through LIF/JAK/STAT may be a common response to Ras/Raf activation in different medullary thyroid carcinoma types. PMID: 20570039
  80. Results describe the effects of 6-h marathon ultra-race on the levels of IL-6, leukemia inhibiting factor (LIF), and stem cell growth factor (SCF) in athletes. PMID: 20396801
  81. Leukemia inhibitory factor is dysregulated in the endometrium and uterine flushing fluid of women with adenomyosis during the implantation window. PMID: 19361790
  82. LIF protein is expressed in Alzheimer's and Parkinson diseases. PMID: 20074285
  83. Studies indicate potential of LIF/IL11 as contraceptive targets and therapeutic agents for infertility. PMID: 19647472
  84. Oncostatin M and leukemia inhibitory factor regulate the growth of normal human breast epithelial cells PMID: 11811789
  85. LIF does not activate STAT3, ERK, or gp130 receptor in human N tera-2/D1 EC cells. The suppressor of cytokine signaling 1 (SOCS-1) is constitutively expressed, suggesting that LIF signal transduction is inhibited by elevated levels of SOCS-1 expression. PMID: 11855863
  86. experimental N-Myc overexpression results in down-regulation of leukemia inhibitory factor (LIF), a modulator of endothelial cell proliferation PMID: 12153570
  87. LIF stimulates (45)Ca release and enhances expression of receptor activator of NF-kappa B ligand (RANKL) and osteoprotegerin in neonatal mouse calvarial cultures, but has no effect on the expression of RANK. PMID: 12218157
  88. role for LIF in loss of autocrine prolactin suppression contributing to unrestrained prolactinomas prolactin secretion. PMID: 12574225
  89. LIF regulates cell survival of normal human endometrial stromal cells dose-dependently. PMID: 12579339
  90. Leukemia inhibitory factor (LIF), cardiotrophin-1, and oncostatin M share structural binding determinants in the immunoglobulin-like domain of LIF receptor PMID: 12707269
  91. LIF has a role in src family kinase-independent signal transduction and gene induction PMID: 12764151
  92. Leukemia inhibitory factor (LIF) increased overall expansion of human neural precursor cells grown in culture; Following LIF withdrawal, 200 genes showed significant decreases PMID: 12807438
  93. binding of ETS transcription factors to the ETS binding sites in the human LIF promoter is critical for its inducibility in response to T cell activators and play an important functional role within the endocrine-immune network. PMID: 14557674
  94. role in hepatocyte growth factor expression PMID: 14647442
  95. Decreased concentartion of LIF in infertile women do not seem to have a genetic basis due to the low frequency of LIF gene alterations. PMID: 14687743
  96. Constitutive expression of cytokines in brain induces changes in gene expression characteristic of chronic inflammation leading to either temporal weight reduction (CNTF) or severe cachexia (leukemia inhibitory factor). PMID: 14715713
  97. immunocytochemical staining and mRNA expression of LIF and its receptor are consistent with the concept that LIF might be involved in growth initiation of primordial follicles through its receptor PMID: 15044601
  98. At early human post-implantation stage, LIF is produced from decidua and chorionic villi and may exert its action on trophoblasts. Anembryonic pregnancy cannot be accounted for by defective expression of either LIF or LIF-Rbeta in most circumstances. PMID: 15180980
  99. Both IL-6 and LIF can be very efficiently induced by IL-1beta in articular chondrocytes in vitro, more so in osteosarthritis than in normal cells. PMID: 15341921
  100. LIFRbeta and the signaling subunit gp130 are expressed in hESCs and human LIF can induce STAT3 phosphorylation and nuclear translocation in hESCs. PMID: 15342941

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Subcellular Location Secreted
Protein Families LIF/OSM family
Database Links

HGNC: 6596

OMIM: 159540

KEGG: hsa:3976

STRING: 9606.ENSP00000249075

UniGene: Hs.2250

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