Recombinant Enterobacteria phage P1 Lysozyme (17)

Code CSB-YP657799EDD
MSDS
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Source Yeast
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Code CSB-EP657799EDD
MSDS
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Source E.coli
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Code CSB-EP657799EDD-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657799EDD
MSDS
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Source Baculovirus
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Code CSB-MP657799EDD
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
17
Uniprot No.
Alternative Names
17; lysa; lyzSAR-endolysin; EC 3.2.1.17; Endolysin; Gene product 17; gp17; Lysis protein; Lysozyme; Muramidase
Species
Escherichia phage P1 (Bacteriophage P1)
Expression Region
1-185
Target Protein Sequence
MKGKTAAGGG AICAIAVMIT IVMGNGNVRT NQAGLELIGN AEGCRRDPYM CPAGVWTDGI GNTHGVTPGV RKTDQQIAAD WEKNILIAER CINQHFRGKD MPDNAFSAMT SAAFNMGCNS LRTYYSKARG MRVETSIHKW AQKGEWVNMC NHLPDFVNSN GVPLRGLKIR REKERQLCLT GLVNE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Signal-arrest-release (SAR) endolysin with lysozyme activity that degrades host peptidoglycans and participates with the pinholin and spanin proteins in the sequential events which lead to programmed host cell lysis releasing the mature viral particles. Once the pinholin has permeabilized the host cell membrane, the SAR-endolysin is released into the periplasm where it breaks down the peptidoglycan layer.
Gene References into Functions
  1. Lyz is activated by an intramolecular thiol-disulfide isomerization; the exported Lyz is kept inactive by 3 levels of control-topological, conformational and covalent-until its release from the membrane is triggered by the P1 holin PMID: 15637279
Subcellular Location
Host cell inner membrane; Single-pass type II membrane protein; Periplasmic side.
Protein Families
Glycosyl hydrolase 24 family
Database Links

KEGG: vg:2777454

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