Recombinant Human Angiogenin(ANG),partial

Code CSB-EP001703HU1
Size US$1726Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names ANG
Uniprot No. P03950
Alternative Names ALS9; AMYOTROPHIC LATERAL SCLEROSIS; ANG 1; ANG; ANG I; ANG1; ANGI; ANGI_HUMAN; Angiogenin; Angiogenin ribonuclease RNase A family; 5; Epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; Ribonuclease RNase A Family 5; RNase 5; RNASE4; RNASE5
Species Homo sapiens (Human)
Source E.coli
Expression Region 26-147aa
Target Protein Sequence DNSRYTHFLTQHYDAKPQGRDDRYCESIMRRRGLTSPCKDINTFIHGNKRSIKAICENKNGNPHRENLRISKSSFQVTTCKLHGGSPWPPCQYRATAGFRNVVVACENGLPVHLDQSIFRRP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 18.0kDa
Protein Length partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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 Q&A
Q:

I have a question “Has cat# CSB-RP057294h been assayed for activity?”

A:
Very nice to receive your inquiry.
We haven't tested the activity of this protein yet. Our protein is purified under mild conditions which should have activity in theory. We suggest you to purchase a small amount to have a try first.

Target Background

Function
(From Uniprot)
Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
Gene References into Functions
  1. Angiogenin contributes to angiogenesis induced by Brain-derived neurotrophic factor (BDNF). PMID: 29573867
  2. Data show that phospholipase D2 (PLD2)-produced phosphatidic acid (PA) promoted cell invasion through the the expression of angiogenin (ANG) in clear cell renal cell carcinoma (ccRCC) cells. PMID: 29660846
  3. The results revealed that PCNA is predominantly localized in the cytoplasm, while hAng is distributed both in the nucleus and in the cytoplasm. hAng and PCNA colocalize in the cytoplasm, suggesting that they may interact in this compartment. PMID: 28777577
  4. RNase 5 suppressed p27 and up-regulated cyclin D1, D3, and E by activating PI3-kinase/Akt in CECs to initiate cell cycle progression PMID: 27526633
  5. When yeast genetic interaction partners held in common between human OPTN and ANG were validated in mammalian cells and zebrafish, MAP2K5 kinase emerged as a potential drug target for amyotrophic lateral sclerosis therapy PMID: 28596290
  6. There was a significant association between RNase 5 and histological differentiation in colon adenocarcinomas, but no association between RNase 5 and Necl 4 in gastric or colon adenocarcinomas PMID: 28561015
  7. Results continue to establish ANG as an oncoprotein and further reveal that ANG contributes to oncogenesis by the activation of MMP2 through modulation of DNMT3b functions. PMID: 27317771
  8. (31) RRR(33) and (50) KRSIK(54) motifs of angiogenin might play a critical role in the regulation of p53-mediated apoptosis and angiogenesis in cancer cells. PMID: 29105754
  9. Study shows that plexin-B2 (PLXNB2) is the functional receptor for ANG in endothelial, cancer, neuronal, and normal hematopoietic and leukemic stem and progenitor cells. PMID: 29100074
  10. Delivery of the therapeutic human genes VEGF and ANG using an adenoviral vector improved functional recovery after traumatic spinal cord injury in a rat model. Immunofluorescent analysis of the post-treatment spinal cord suggested that the positive effect of Ad5-VEGF+Ad5-ANG transduction on recovery of locomotor function may be due to the action of glial cells on motor neurons. PMID: 28452633
  11. Ang down-regulate the expression of Col-I, alpha-SMA and TGF-beta1/Smad2/3 and subsequently inhibits fibroblast-myofibroblast transition. PMID: 27543459
  12. Ang,Tie1 and Tie2 play roles in vascular development and pathogenesis of vascular diseases.[review] PMID: 27941161
  13. Results indicate that angiogenin (ANG) plays a critical role in regulating angiogenesis and follicle survival in xenografted human ovarian tissues. PMID: 28274269
  14. we found that ANG plays an important role in EMT in squamous cell lung carcinoma and may be a valuable therapeutic target for squamous cell lung carcinoma PMID: 27667357
  15. There is Lack of association between the Angiogenin (ANG) rs11701 polymorphism and amyotrophic lateral sclerosis risk. PMID: 26753798
  16. ANG plays a non-cell-autonomous role in regulation of hematopoiesis by simultaneously preserving hematopoietic stem/progenitor cells stemness and promoting myeloid-restricted progenitor cell proliferation. PMID: 27518564
  17. ANG K17I variant is rare in Caucasian patients and controls and increases the risk for amyotrophic lateral sclerosis PMID: 26255299
  18. ANG rs11701 variant is a genetic risk factor for Parkinson's disease in Taiwan population.Mutations in ANG are not a common cause for idiopathic Parkinson's disease. PMID: 25386690
  19. In conclusion, angiogenin secretion by HCCs favors tumor development by inducing HSC activation and ECM remodeling. PMID: 25604905
  20. the present study established a link between Ang and FHL3 proteins and identifies a new pathway for regulating astrocytoma progression. PMID: 25659096
  21. Studied the mechanism of ligand binding in human angiogenin using the NMR chemical shift projection analysis. PMID: 25450558
  22. ANG could play a pivotal role in the development of bladder cancer through regulating AKT/mTOR signaling pathway. PMID: 25564356
  23. Suggest a novel regulatory pathway whereby breast tumor-derived angiogenin directly activates angiogenesis through inhibition of miR-542-3p. PMID: 26272182
  24. a biological explanation for the loss-of-function of D22G-Angiogenin leading to ALS, and suggest that the L35P-Angiogenin mutation would probably cause ALS symptoms in individuals harboring this mutation PMID: 25372031
  25. High angiogenin expression is associated with bladder cancer. PMID: 24696417
  26. a novel mechanism of ANG in regulating PI3K/AKT/mTOR signaling pathway via RI, is reported. PMID: 25193113
  27. Up-regulated ANG expression and increased nuclear translocation were observed in cervical cancers as compared to normal cervical tissues; upregulation of ANG was positively correlated with primary tumor invasion PMID: 25552400
  28. findings support an unrecognized interplay between ANG, ERK1/2 and MMP2 that can impact tumor growth and progression. PMID: 24561529
  29. The observed pattern of angiogenin expression is compatible with a role in blood vessel formation and in cross-talk between trophoblasts and endothelial cells. PMID: 25093183
  30. Serum angiogenin levels may be useful to distinguish between cancer and noncancer patients among the candidates for prostatic biopsy in regular clinical practice. PMID: 23921786
  31. ANG interacts with plasminogen activation system at the leading edges of breast cancer cell surfaces and facilitates interactions of uPAR with uPA to regulate plasmin formation and cell migration. PMID: 24457100
  32. Amyloidogenic ApoA-I induces cell death is through attenuating anti-stress activity of angiogenin. PMID: 24603325
  33. Angiogenin was up-regulated in proliferative hemangiomas. Angiogenin serum levels correlate with the hemangioma stage. Angiogenin levels discriminate between proliferative hemangiomas and the control group and patients with venous malformations. PMID: 25068345
  34. Depletion of cellular ANG expression abolished PLSCR1-enhanced rRNA transcription. PMID: 24356419
  35. Human angiogenin has antimicrobial activity against C. albicans and S. pneumoniae. PMID: 12548285
  36. Data show that the transcription of angiogenin (ANG) and ribonuclease 4 (RNASE4) promoter is influenced by RNA polymerase III (Pol III) elements and could be differentially regulated by an intragenic CCCTC binding factor (CTCF)-dependent chromatin loop. PMID: 24659782
  37. Data shows ANG-stimulated rRNA transcription is not only an essential component for androgen-dependent growth of prostate cancer but also contributes to the transition of prostate cancer from androgen-dependent to castration-resistant growth status. PMID: 23851444
  38. Nuclear ANG directly binds to the ANG-Binding Sequence within ERRgamma of ERRgamma gene and inhibits ERRgamma transcription to promote breast cancer cell proliferation. PMID: 23977052
  39. These data suggest that one of the mechanisms by which ANG stimulates rRNA transcription is through an epigenetic activation of rDNA promoter. PMID: 24122807
  40. Changes of angiogenin in the skin of patients with amyotrophic lateral sclerosis are related to the disease process. PMID: 23351638
  41. Overexpression of ANG in pterygium body fibroblasts might be involved in active pterygium growth with thick pterygium body formation and increased risk of recurrence. PMID: 23989187
  42. Our data do not support the association of angiogenin variants with Parkinson's disease in Han Chinese of mainland China. PMID: 23231972
  43. Increased angiogenin may contribute to decreased T-cell zeta-chain expression in hemodialysis patients PMID: 23379493
  44. fast molecular dynamics based method for determining the mechanisms of functional loss caused by mutations PMID: 23665167
  45. Angiogenin is up-regulated in the hypoxic environment of oral squamous cell carcinoma PMID: 22694909
  46. Dysregulated angiogenin expression may contribute to the pathogenesis of myositis as well as skin involvement via the vascular change in DM/CADM PMID: 23229115
  47. analysis of Angiogenin in middle-aged type 1 diabetes patients PMID: 22940420
  48. Angiogenin interacts with p53 and colocalizes in the nucleus. PMID: 22266868
  49. In Alzheimer disease, serum angiogenin was decreased and cognitive function was associated with correlated with angiogenin levels. PMID: 22449478
  50. serum angiogenin levels in cutaneous T cell lymphoma (CTCL)patients were significantly higher than healthy controls; angiogenin mRNA expression levels in lesional skin were elevated in erythrodermic CTCL compared to normal skin; results suggest enhanced angiogenin expression may be related with poor prognosis of erythrodermic CTCL PMID: 22526325

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Involvement in disease Amyotrophic lateral sclerosis 9 (ALS9)
Subcellular Location Cytoplasmic vesicle, secretory vesicle lumen, Secreted, Nucleus, Nucleus, nucleolus
Protein Families Pancreatic ribonuclease family
Tissue Specificity Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
Database Links

HGNC: 483

OMIM: 105850

KEGG: hsa:283

STRING: 9606.ENSP00000336762

UniGene: Hs.283749

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