Human Angiogenin,ANG ELISA Kit

Code CSB-E04498h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P03950
Lead Time 3-5 working days
Abbreviation ANG
Protein Biological Process 1 Cardiovascular
Target Name angiogenin, ribonuclease, RNase A family, 5
Alias ALS9, HEL168, MGC22466, MGC71966, RNASE4, RNASE5, OTTHUMP00000164021|epididymis luminal protein 168
Species Homo sapiens (Human)
Protein Biological Process 3 Angiogenesis
Sample Types serum, plasma, cell culture supernates, urine, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5 exons. Each gene splices to a unique downstream exon that contains its complete coding region.
HGNC 483
RGD 1359373
MGI 88022
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ANG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:200Average %88
Range %84-92
1:400Average %94
Range %90-98
1:800Average %99
Range %95-104
1:1600Average %102
Range %96-108
Recovery
The recovery of human ANG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8993-96
EDTA plasma (n=4)10095-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10001.865 1.830 1.848 1.686
5001.457 1.446 1.452 1.290
2501.128 1.082 1.105 0.943
1250.693 0.708 0.701 0.539
62.50.437 0.409 0.423 0.261
31.20.260 0.243 0.252 0.090
15.60.209 0.201 0.205 0.043
00.163 0.160 0.162  
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Function Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
Involvement in disease Amyotrophic lateral sclerosis 9 (ALS9)
Subcellular Location Cytoplasmic vesicle, secretory vesicle lumen, Secreted, Nucleus, Nucleus, nucleolus
Protein Families Pancreatic ribonuclease family
Tissue Specificity Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
Database Links

HGNC: 483

OMIM: 105850

KEGG: hsa:283

STRING: 9606.ENSP00000336762

UniGene: Hs.283749

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