nes Antibody

Code CSB-PA893810XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) nes Polyclonal antibody
Uniprot No.
Target Names
nes
Alternative Names
nes antibody; nessy antibody; CG9655Lysophospholipid acyltransferase 5 antibody; LPLAT 5 antibody; 1-acylglycerophosphocholine O-acyltransferase antibody; EC 2.3.1.23 antibody; 1-acylglycerophosphoethanolamine O-acyltransferase antibody; EC 2.3.1.n7 antibody; 1-acylglycerophosphoserine O-acyltransferase antibody; EC 2.3.1.n6 antibody; Lysophosphatidylcholine acyltransferase antibody; LPCAT antibody; Lyso-PC acyltransferase antibody; Lysophosphatidylethanolamine acyltransferase antibody; LPEAT antibody; Lyso-PE acyltransferase antibody; Lysophosphatidylserine acyltransferase antibody; LPSAT antibody; Lyso-PS acyltransferase antibody; Membrane-bound O-acyltransferase domain-containing protein 5 antibody; O-acyltransferase domain-containing protein 5 antibody; Protein nessy antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) nes protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acyltransferase that mediates the acylation of lysophospholipids to produce phospholipids (glycerophospholipids). Highest activity with lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) producing phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (LPCAT activity), but also converts lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) to phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity). Has a preference for unsaturated fatty acids of at least 16 carbons such as oleoyl-CoA ((9Z)-octadecenoyl-CoA) and palmitoleoyl-CoA ((9Z)-hexadecenoyl-CoA). Glycerophospholipids are important structural and functional components of cellular membrane, acyl-chain remodeling regulates the molecular species distribution of glycerophospholipids which can affect membrane fluidity and curvature. Essential for fertility and viability together with Oysgedart (Oys). Required for germ cells to migrate into the mesoderm.
Gene References into Functions
  1. The Drosophila homologues of membrane-bound O-acyltransferase, Oysgedart (Oys), Nessy (Nes), and Farjavit (Frj), are lysophospholipid acyltransferases. PMID: 19864461
Subcellular Location
Endoplasmic reticulum. Membrane; Multi-pass membrane protein.
Protein Families
Membrane-bound acyltransferase family
Tissue Specificity
During gastrulation, expressed mainly along the midline in the presumptive mesoderm. During germ band elongation, expressed in mesoderm and endoderm primordia and in the cephalic furrow. Expression in mesoderm and endoderm lineages continues during germ b
Database Links

KEGG: dme:Dmel_CG9655

STRING: 7227.FBpp0074786

UniGene: Dm.2523

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