Mouse Angiotensin converting enzyme,ACE ELISA Kit

Code CSB-E04492m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P09470
Lead Time 3-5 working days
Abbreviation ACE
Target Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Alias ACE1, CD143, DCP, DCP1, MGC26566, MVCD3, CD143 antigen|angiotensin I converting enzyme 1|angiotensin converting enzyme, somatic isoform|carboxycathepsin|dipeptidyl carboxypeptidase 1|kininase II|pep
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 95  
Range % 84-101  
1:10 Average % 96  
Range % 86-103  
1:20 Average % 96  
Range % 92-100  
1:40 Average % 95  
Range % 87-101  
Recovery
The recovery of mouse ACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-103  
EDTA plasma (n=4) 92 85-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.153 2.141 2.147 1.971  
50 1.542 1.596 1.569 1.393  
25 1.117 1.104 1.111 0.935  
12.5 0.703 0.688 0.696 0.520  
6.25 0.489 0.474 0.482 0.306  
3.12 0.322 0.318 0.320 0.144  
1.56 0.259 0.266 0.263 0.087  
0 0.172 0.180 0.176    
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Function Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.
Subcellular Location Angiotensin-converting enzyme, soluble form: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families Peptidase M2 family
Tissue Specificity Testis-specific isoform is expressed in spermatocytes, adult testis.
Database Links

KEGG: mmu:11421

STRING: 10090.ENSMUSP00000001963

UniGene: Mm.754

Pathway Renin-angiotensin system

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