Mouse Angiotensin converting enzyme,ACE ELISA Kit

Code CSB-E04492m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Alternative Names ACE1, CD143, DCP, DCP1, MGC26566, MVCD3, CD143 antigen|angiotensin I converting enzyme 1|angiotensin converting enzyme, somatic isoform|carboxycathepsin|dipeptidyl carboxypeptidase 1|kininase II|pep
Abbreviation ACE
Uniprot No. P09470
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 95  
Range % 84-101  
1:10 Average % 96  
Range % 86-103  
1:20 Average % 96  
Range % 92-100  
1:40 Average % 95  
Range % 87-101  
The recovery of mouse ACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-103  
EDTA plasma (n=4) 92 85-98  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.153 2.141 2.147 1.971  
50 1.542 1.596 1.569 1.393  
25 1.117 1.104 1.111 0.935  
12.5 0.703 0.688 0.696 0.520  
6.25 0.489 0.474 0.482 0.306  
3.12 0.322 0.318 0.320 0.144  
1.56 0.259 0.266 0.263 0.087  
0 0.172 0.180 0.176    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.
Subcellular Location Angiotensin-converting enzyme, soluble form: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families Peptidase M2 family
Tissue Specificity Testis-specific isoform is expressed in spermatocytes, adult testis.
Database Links

KEGG: mmu:11421

STRING: 10090.ENSMUSP00000001963

UniGene: Mm.754

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