Mouse Angiotensin converting enzyme,ACE ELISA Kit

Instructions
Code CSB-E04492m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Alternative Names Ace ELISA kit; Dcp1Angiotensin-converting enzyme ELISA kit; ACE ELISA kit; EC 3.2.1.- ELISA kit; EC 3.4.15.1 ELISA kit; Dipeptidyl carboxypeptidase I ELISA kit; Kininase II ELISA kit; CD antigen CD143) [Cleaved into: Angiotensin-converting enzyme ELISA kit; soluble form] ELISA kit
Abbreviation ACE
Uniprot No. P09470
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 95  
Range % 84-101  
1:10 Average % 96  
Range % 86-103  
1:20 Average % 96  
Range % 92-100  
1:40 Average % 95  
Range % 87-101  
Recovery
The recovery of mouse ACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-103  
EDTA plasma (n=4) 92 85-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.153 2.141 2.147 1.971  
50 1.542 1.596 1.569 1.393  
25 1.117 1.104 1.111 0.935  
12.5 0.703 0.688 0.696 0.520  
6.25 0.489 0.474 0.482 0.306  
3.12 0.322 0.318 0.320 0.144  
1.56 0.259 0.266 0.263 0.087  
0 0.172 0.180 0.176    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.
Gene References into Functions
  1. ACE expression/phosphorylation in the bone-marrow niche interface negatively regulates G-CSF-induced signaling and hematopoietic progenitor cell mobilization. PMID: 29549541
  2. first evidence indicating the causation between ACE DD or B2R+9bp genotype and the increased risk for diabetic nephropathy, broadening our horizon about the role of genetic modulators in this disease. PMID: 28390948
  3. a small increase in angiotensin-converting enzyme activity in diabetic animals leads to greater impairment of autonomic function, as demonstrated by increased sympathetic modulation and reduced cardiac vagal modulation along with increased renal expression of Ang II. PMID: 30088535
  4. Resveratrol upregulated ACE2 and inhibited abdominal aortic aneurysm growth in a mouse model. PMID: 28935757
  5. In mice with renal injury induced by L-NAME pretreatment, renal tubular epithelial ACE is essential for renal angiotensin II accumulation and salt-sensitive hypertension PMID: 27988209
  6. Studied ACE role in peptide processing and for MHC class II antigen presentation; found ACE level effects efficiency of antigen presentation, and overexpression or inhibition of ACE alters the CD4(+) T-cell and antibody response. PMID: 28394320
  7. ACE enhances the oxidative response and bactericidal activity of neutrophils. PMID: 28515091
  8. this study shows that angiotensin-converting enzyme inhibitor captopril rescues mice from endotoxin-induced lethal hepatitis PMID: 27879415
  9. Smooth muscle cell-derived ACE contributes to atherosclerosis, independent of circulating ACE activity and blood pressure. PMID: 27055902
  10. Intestinal ACE shedding is increased by DSS-induced intestinal inflammation and parallels local corticosterone production. ACE product angiotensin II stimulates corticosterone formation in healthy intestine. PMID: 26401072
  11. Renin angiostensin system contributes to 20-HETE-mediated microvascular remodeling in hypertension and that 20-HETE-driven microvascular remodeling independent of blood pressure elevation does not fully rely on ACE activity in the vascular endothelium. PMID: 25924878
  12. angiotensin-converting enzyme has an essential role in hypertension induced by nitric oxide synthesis inhibition PMID: 25012170
  13. Suggest that the ACE2-ACE imbalance plays an important role in the pathogenesis of severe acute pancreatitis and that pancreatic ACE2 is an important factor in determining the severity of SAP. PMID: 24414175
  14. Show that tissue-specific targets are critical for the effects of miR-143/145 on smooth muscle differentiation and that angiotensin converting enzyme is one such target. PMID: 25273883
  15. Tissue-specific expression of transgenic secreted ACE in vasculature can restore normal kidney functions, but not blood pressure, of Ace-/- mice. PMID: 24475296
  16. ACE-null mice produce large numbers of myeloid-derived suppressor cells during chronic inflammation. PMID: 24614194
  17. Myelomonocytic expression of catalytically active ACE, prevents cognitive decline in a murine model of Alzheimer disease. PMID: 24487585
  18. ACE mediates angiotensin I-induced atherosclerosis, and ACE expression in leukocytes modestly contributes to atherosclerotic development in hypercholesterolemic mice. PMID: 23846498
  19. TEX101 is a unique specific substrate for ACE that is essential for the production of fertile mouse spermatozoa PMID: 23633567
  20. renal ACE activity is required to increase local Ang II, to stimulate sodium transport in loop of Henle and the distal nephron, and to induce hypertension PMID: 23619363
  21. deregulation of ACE and ACE2 plays an important role in tourniquet-induced renal injury PMID: 22580272
  22. When Ang I and CMP48/80 were co-administered, AT(1B) receptor expression was increased, MCP-4 was found surrounding the vessel wall PMID: 21622682
  23. shapes the MHC class I peptide repertoire for antigen presentation to T-cells PMID: 21964607
  24. Mature adipocytes modulate the expression profile of macrophages by releasing lipid mediators that increase ACE expression. PMID: 21116821
  25. results demonstrate a previous unrecognized significant role for ACE in myelopoiesis and imply new perspectives for manipulating myeloid cell expansion and maturation. PMID: 21148418
  26. Leptin regulates ACE activity in mice PMID: 20614101
  27. Angiotensin-converting enzyme overexpression in mouse myelomonocytic cells augments resistance to Listeria and methicillin-resistant Staphylococcus aureus PMID: 20937811
  28. A modest genetic increase in ACE impairs myocardial tolerance to ischemia. ACE level plays a critical role in cardiac ischemia, through both kinin and angiotensin mediated mechanisms. PMID: 20667972
  29. data provide direct evidence that small genetic disturbances in ACE levels per se have an influence on haemodynamic, cardiac mass and autonomic nervous system responses in mice under pathological perturbation PMID: 19930431
  30. angiotensin-converting enzyme has a role in improving autonomic dysregulation in ob/ob mice PMID: 20012594
  31. data suggest that the two catalytic domains of mACE do not function independently but that the signal transduction is influenced by negative cooperativity of the two catalytic domains PMID: 20030584
  32. There is a switch from ACE-dependent to serine protease-dependent ANG II formation in the type II diabetic kidney. PMID: 19846569
  33. data suggest that ACE is essential to ANG II formation in the vascular space PMID: 12003835
  34. urine-concentrating defect in mice with absence of tissue ACE is associated with downregulation of key urea, salt, and water transport proteins PMID: 12167603
  35. Chymase may play a role in heart remodeling by increasing Ang II formation and activating MMP-9, and the regulation of collagen I gene expression. PMID: 12359984
  36. gender differences in expression of ACE in the heart PMID: 12384478
  37. results demonstrated that the rodent germinal angiotensin converting enzyme is released from the testicular sperm membrane when sperm enter the epididymis PMID: 12444051
  38. There is no any association between angiotensin converting enzyme polymorphism and Alzheimer's disease. PMID: 12459519
  39. Substantial evidence is provided for a major role for angiotensin converting enzyme in a murine allergic contact dermatitis model of neurogenic infolammation. PMID: 12646655
  40. Expression of ACE, either in renal proximal tubules or in vasculature, is sufficient for maintaining normal kidney functions, but for maintaining blood pressure, ACE must be expressed in vascular endothelial cells. PMID: 12777443
  41. the presence of the C-terminal ACE catalytic domain is sufficient to maintain a functional renin-angiotensin system PMID: 14757757
  42. ACE is the predominant pathway of angiotensin II formation in blood and tissues of mice and plays a major role in bradykinin (1-9) metabolism in blood and, to a lesser extent, in kidney and heart. PMID: 14769811
  43. t-ACE contributes to arterial structure/remodeling & hyperplastic carotid inward remodeling from blood flow cessation. It is not needed for outward hypertrophic & subsequent inward hypotrophic remodeling of the uterine artery during/after pregnancy. PMID: 15031129
  44. Aldosterone upregulated macrophage ACE mRNA expression and activity. The selective effect of aldosterone on tissue ACE, versus circulating ACE, further demonstrates differences in the regulation pathways of tissue and circulating ACE. PMID: 15123520
  45. ACE Tyr phosphorylation, probably in the endoplasmic reticulum, enhances the rate of its cleavage secretion at the plasma membrane using a regulatory pathway that may involve p38 MAP kinase PMID: 15252021
  46. Mice with cardiac-restricted angiotensin-converting enzyme have atrial enlargement, cardiac arrhythmia, and sudden death. PMID: 15331425
  47. Ptn has the potential to critically regulate the downstream activities of angiotensin II, through the regulation of its synthesis by ACE and its receptor mediated functions through regulation of both the AT1 and AT2 receptors PMID: 15485659
  48. renal tissue angiotensin converting enzyme is an important contributor to tubuloglomerular feedback PMID: 15494545
  49. ACE is associated with body weight and peri-epididymal fat accumulation PMID: 15522949
  50. ACE signaling may underlie the increase in COX-2 and prostacyclin levels in patients treated with ACE inhibitors. PMID: 15569856
  51. a new function for ACE; it has a GPI-anchored protein releasing activity and has a crucial role in fertilization through this activity PMID: 15665832
  52. regional distribution of ACE in the brain PMID: 16154999
  53. This study shows a previously undescribed expression of angiotensin converting enzyme by intraepithelial lymphocytes. PMID: 16215678
  54. Male fertility is dependent on dipeptidase activity of testis ACE. PMID: 16270063
  55. Enzymatic activity examined in knockout mice in a rodent model of diabetes, in the db/db and streptozotocin (STZ)-induced diabetic mice in kidney cortex. PMID: 16804085
  56. Identify ACE as a previously unrecognized marker of PCFCs and suggest that ACE is functionally important for PCFC proliferation. PMID: 16857898
  57. beta-amyloid levels in the brain are regulated by neprilysin and endothelin-converting enzyme but not angiotensin-converting enzyme PMID: 16912050
  58. Collectively, these data provide direct evidence that ACE gene dosage per se does not influence cardiac mass but upon a pathological stimulus, such as elevation in blood pressure, it modulates cardiac mass in both mice and humans. PMID: 16926271
  59. Collectively, these data indicate that small isolated genetic disturbances in ACE cardiac levels can be well compensated under physiological perturbations PMID: 16926272
  60. These results suggest that ACE negatively regulate the myogenesis through the mechanism at least in part via production of angiotensin II followed by its binding to AT2 receptor. PMID: 17188239
  61. Suggest that angiotensin II, formed by the enzymatic activity of angiotensin converting enzyme and chymase, plays an important role in inducing postischemic leukocyte rolling/adhesion in mouse model of intestinal ischemia. PMID: 17307998
  62. Therefore, cardiac-specific ACE overexpression resulted in changes in connexins consistent with the phenotype of low-voltage electrical activity. PMID: 17337599
  63. Results show that mice with enhanced macrophage angiotensin-converting enzyme are resistant to melanoma. PMID: 17525278
  64. ACE mRNA levels increased three-fold in Npr1 knockout mice compared with wild-type. PMID: 17566078
  65. Testis ACE contributes to fertilization as a dipeptidase at least in the epididymis. PMID: 17634445
  66. Results suggest that ACE negatively regulates the myotube maturation via impairment of mTOR function. PMID: 17892857
  67. both wild-type and heterozygous mice develop similar degrees of cardiac hypertrophy after aortic banding PMID: 18032521
  68. Data describe the role of the ACE/AT2R complex in the control of mesenteric artery contraction induced by ACE/AT1R complex activation in response to Ang I. PMID: 18084722
  69. C-domain of angiotensin-converting enzyme plays the major role in the conversion of Angiotensin I to angiotensin II. PMID: 18158355
  70. ACE/kinin B(2) receptor interaction modulates angiotensin-converting enzyme (ACE) activity. PMID: 18212275
  71. Bradykinin receptors (B2R and B1R) contribute to the cardioprotective bradykinin-mediated effect of ACE inhibition. PMID: 18347228
  72. The decreased body fat in ACE(-/-) mice is independent of food intake and appears to be due to a high energy expenditure related to increased metabolism of fatty acids in the liver, with the additional effect of increased glucose tolerance. PMID: 18443281
  73. ACE appears to have an important role in the modulation of intestinal EC apoptosis and proliferation and suggests that the presence of ACE in the intestinal epithelium has a critical role in guiding epithelial cell adaptive response PMID: 18483182
  74. localized in the endothelial layer in kidney arterioles PMID: 19004932
  75. Endogenous ACE-mediated Ang II formation plays a significant role in the increases of blood pressure and intrarenal Ang II during Ang II-induced hypertension. PMID: 19075090
  76. Exercise training decreased alpha(2A)/alpha(2C)adrenoceptor lacking mice's cardiac angiotensin II levels and angiotensin-converting enzyme activity. PMID: 19125280
  77. Data indicate that small physiologically relevant changes in ACE, not associated with basal vascular abnormalities or blood pressure levels, do influence the magnitude of cuff-induced neointima thickening. PMID: 19258495
  78. Just after stopping the angiotensin II infusion, aortic ACE and chymase activities were significantly increased, and were involved in the progression of aortic abdominal aneurysms PMID: 19556720
  79. The vascular compartment is the main site of AngI and bradykinin-(1-9) formation and metabolism and vascular ACE may limit AngI entry to the extravascular compartment of WT mice. PMID: 19673938
  80. Ace is a candidate gene for survival in acute lung injury. PMID: 17656683

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Subcellular Location Angiotensin-converting enzyme, soluble form: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families Peptidase M2 family
Tissue Specificity Testis-specific isoform is expressed in spermatocytes, adult testis.
Database Links

KEGG: mmu:11421

STRING: 10090.ENSMUSP00000001963

UniGene: Mm.754

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