Human clusterin,CLU ELISA Kit

Code CSB-E09121h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P10909
Lead Time 3-5 working days
Abbreviation CLU
Protein Biological Process 1 Apoptosis/Autophagy
Target Name clusterin
Alias AAG4, APOJ, CLI, KUB1, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, aging-associated protein 4|apolipoprotein J|clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, cell culture supernates, saliva, urine
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 29094
RGD 1307222
MGI 1921398
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %90
Range %85-96
1:2000Average %97
Range %91-104
1:4000Average %92
Range %85-100
1:8000Average %93
Range %83-99
Recovery
The recovery of human CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-106
EDTA plasma (n=4)9590-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.248 2.205 2.227 2.072
1001.196 1.266 1.231 1.076
500.675 0.663 0.669 0.514
250.472 0.453 0.463 0.308
12.50.335 0.314 0.325 0.170
6.250.221 0.207 0.214 0.059
3.120.200 0.194 0.197 0.042
00.155 0.154 0.155  
References
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Function Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
Subcellular Location Isoform 1: Secreted, Note=Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress, SUBCELLULAR LOCATION: Nucleus, Cytoplasm, Mitochondrion membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, cytosol, Microsome, Endoplasmic reticulum, Cytoplasmic vesicle, secretory vesicle, chromaffin granule
Protein Families Clusterin family
Tissue Specificity Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain,
Database Links

HGNC: 2095

OMIM: 185430

KEGG: hsa:1191

STRING: 9606.ENSP00000315130

UniGene: Hs.436657

Pathway Complement and coagulation cascades

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