Human clusterin,CLU ELISA Kit

Instructions
Code CSB-E09121h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name clusterin
Alternative Names 40 ELISA Kit; AAG 4 ELISA Kit; AAG4 ELISA Kit; Aging associated protein 4 ELISA Kit; Aging-associated gene 4 protein ELISA Kit; AI893575 ELISA Kit; APO J ELISA Kit; Apo-J ELISA Kit; APOJ ELISA Kit; ApoJalpha ELISA Kit; ApoJbeta ELISA Kit; Apolipoprotein J ELISA Kit; ApolipoproteinJ ELISA Kit; CLI ELISA Kit; CLU ELISA Kit; CLU1 ELISA Kit; CLU2 ELISA Kit; CLUS_HUMAN ELISA Kit; Clusterin alpha chain ELISA Kit; Clusterin ELISA Kit; Clusterin beta chain ELISA Kit; Complement associated protein SP 40 40 ELISA Kit; Complement associated protein SP 40 ELISA Kit; Complement associated protein SP40 ELISA Kit; Complement cytolysis inhibitor a chain ELISA Kit; Complement cytolysis inhibitor ELISA Kit; Complement cytolysis inhibitor b chain ELISA Kit; Complement lysis inhibitor ELISA Kit; Complement-associated protein SP-40 ELISA Kit; D14Ucla3 ELISA Kit; Dimeric acid glycoprotein ELISA Kit; Glycoprotein 80 ELISA Kit; Glycoprotein III ELISA Kit; GP80 ELISA Kit; Ku70-binding protein 1 ELISA Kit; KUB 1 ELISA Kit; KUB1 ELISA Kit; MGC24903 ELISA Kit; NA1/NA2 ELISA Kit; RATTRPM2B ELISA Kit; SGP 2 ELISA Kit; SGP2 ELISA Kit; SP 40 ELISA Kit; SP40 ELISA Kit; Sugp-2 ELISA Kit; Sulfated glycoprotein 2 ELISA Kit; Testosterone repressed prostate message 2 ELISA Kit; Testosterone-repressed prostate message 2 ELISA Kit; TRPM 2 ELISA Kit; TRPM-2 ELISA Kit; TRPM2 ELISA Kit; TRPM2B ELISA Kit; Trpmb ELISA Kit
Abbreviation CLU
Uniprot No. P10909
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, saliva, urine
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %90
Range %85-96
1:2000Average %97
Range %91-104
1:4000Average %92
Range %85-100
1:8000Average %93
Range %83-99
Recovery
The recovery of human CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-106
EDTA plasma (n=4)9590-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.248 2.205 2.227 2.072
1001.196 1.266 1.231 1.076
500.675 0.663 0.669 0.514
250.472 0.453 0.463 0.308
12.50.335 0.314 0.325 0.170
6.250.221 0.207 0.214 0.059
3.120.200 0.194 0.197 0.042
00.155 0.154 0.155  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Customer Reviews
  • Tested application: ELISA
    Test sample: Serum
    Species: Human
    Review: This kit is quite easy to perform, even for people not familiarised with this kind of procedures. Personally, I had some problems obtaining high levels of absorbance at 450 nm in some samples. This problem happened only in some duplicates, so it probably was due to a human mistake (my mistake).

Target Data

Function Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
Gene References into Functions
  1. The plasma levels of septin-9 and clusterin in ovarian cancer patients were abnormally elevated, which might be used as potential candidates of peripheral blood tumor biomarkers for early diagnosis of EOC and septin-9 might be related to distal metastases of EOC. PMID: 29970704
  2. Data suggest that the potential of clusterin and glutathione synthetase (GSH-S) as platelet biomarkers for early detection of colorectal cancer (CRC) could improve existing screening modalities in clinical application. PMID: 28849249
  3. recombinant alpha- and beta-chains exhibit structural and functional differences and differ in their sub-cellular localization. PMID: 28120874
  4. miR-195 improved the sensitivity of resistant prostate cancer cells to docetaxel by suppressing CLU. PMID: 29665645
  5. The VDR/MEG3/Clusterin signaling pathway may serve as potential therapeutic targets and prognosis biomarkers for colorectal cancer patients in future. PMID: 29628342
  6. Meta analysis validated the Alzheimer disease protective association for CLU (rs11136000) variants. PMID: 29504051
  7. that the Clusterin rs11136000 polymorphism C allele is associated with AD susceptibility PMID: 29396813
  8. clusterin promotes growth and invasion in renal cell carcinoma cells in vitro and in vivo through upregulation of S100A4 PMID: 29400663
  9. The results showed that secreted CLU was overexpressed in three hepatocellular carcinoma cell lines. The downregulation of CLU by CLU shRNA synergistically increased Sorafenib sensitivity in the Bel7402 and SMMC7721 cells, and potentiated Sorafenib induced cell apoptosis. PMID: 29436591
  10. Our study showed low clusterin immunostaining in colorectal carcinoma with lack of association with prognostic indicators PMID: 29279586
  11. This study showed that the 1- and 2-year follow-up, generalized estimating equation analyses of Alzheimer's disease patients revealed that high levels of plasma clusterin at baseline were associated with a significantly larger decrease in Mini-Mental State Examination compared with low levels of plasma clusterin. PMID: 29169407
  12. plasma level decreases in healthy but not in asthmatic pregnancy and correlates directly with lung function PMID: 29200898
  13. Downregulation of apoE and apoJ in CSF strongly suggests a critical role of lipid metabolism in the development and progression of Moyamoya disease. PMID: 28843803
  14. Our study may help to further elucidate the development and progression of non-small cell lung cancer , also it may contribute to the research of therapies targeting sCLU. PMID: 28954633
  15. Using global proteomic profiling of brain leptomeningeal arteries, this study revealed that clusterin and tissue inhibitor of metalloproteinases-3 increase in leptomeningeal arteries affected by cerebral amyloid angiopathy. PMID: 27543695
  16. The presence of microbial invasion of the amniotic cavity, intra-amniotic inflammation (IAI), and microbial-associated IAI was characterized by lower amniotic fluid clusterin concentrations in pregnancies complicated by preterm prelabor rupture of membranes. PMID: 27806672
  17. Our meta-analysis demonstrated that the rs9331888/C> G polymorphism in the clusterin gene might contribute to Alzheimer disease susceptibility especially in Caucasian populations. PMID: 28168383
  18. these findings revealed that CLU genotypes could probably modulate the cerebral the Abeta loads on imaging and volume of hippocampus PMID: 27229352
  19. No association for CLU with Alzheimer's disease in south-Indian population. PMID: 28558900
  20. we confirm that a PEX/Alzheimer's Disease associated risk variant, rs2279590, resides within an enhancer element and regulates the expression of three candidate genes CLU, PTK2B and EPHX2, which were previously known to be modulators in the progression of Alzheimer's Disease. PMID: 28973302
  21. Silencing the expression of CLU could improve the anticancer efficacy of CPT. PMID: 28064403
  22. Thus, we suggest that a combination of PI3K/AKT/mTOR inhibitors with V-ATPase inhibitors might be an effective approach for NSCLC treatment. PMID: 29253572
  23. plasma clusterin was associated with lower volume of the entorhinal cortex, an area that atrophies early in AD. Clusterin could be informative as part of a multi-component preclinical marker for AD PMID: 29324756
  24. This study demonstrated that Apo J expression preferentially occurs in neurons in brain in patient with Alzheimer's Disease. PMID: 27197790
  25. Hippocampal shape features derived from the diffeomorphic metric-based shape analysis led to the identification of significant CLU-PICALM interaction effects on hippocampal morphology in young healthy adults, which were not identified by volume measurement and voxel-wise analysis. PMID: 27017968
  26. CLU-C allele contrubutes to architectural disruptions in resting-state networks in amnestic mild cognitive impairment (aMCI) subjects (i.e., individuals with elevated risk of AD). PMID: 26899953
  27. A total of 249 epileptic patients and 289 healthy controls were included in this study. Three Clusterin single nucleotide polymorphisms (SNPs: rs11136000, rs9314349, and rs9331949) were selected and genotyped with the SNaPshot assay. Our study failed to detect an association between Clusterin polymorphisms (rs11136000, rs9314349, and rs9331949) and epilepsy in a Han Chinese population. PMID: 28972394
  28. Subarachnoid hemorrhage is associated with immediate decrease in CSF clusterin concentrations. PMID: 28803177
  29. Serum ApoJ levels are closely correlated with the magnitude of insulin resistance. PMID: 28986164
  30. data confirm that the association of psoriatic disease with some comorbidities, especially metabolic and cardiovascular disease, might support the correlation with increased circulating Clu. PMID: 28958138
  31. blood platelets have a role in amyloid-beta aggregation in cerebral vessels through integrin alphaIIbbeta3-induced outside-in signaling and clusterin release PMID: 27221710
  32. The results identify Clusterin as a new molecular partner involved in apoptotic cell efferocytosis and suggest a protective role for Clusterin in inflammation and autoimmune diseases. PMID: 27148688
  33. CLU as novel and promising biomarker for prognosis in patients with chronic HF PMID: 28391884
  34. FRET and co-IP assays demonstrated that Clu interacted with beta-amyloid peptide, a pathological protein of AD, which suggested a potential effect of SelR and Abeta with the aid of Clu. The interaction between SelR and Clu provides a novel avenue for further study on the mechanism of SelR in AD prevention. PMID: 23805218
  35. The findings of this study provided further evidence for the CLU risk variant as a candidate gene for Alzheimer disease and may serve as a pre-clinical neuroimaging phenotype of late-onset Alzheimer disease. PMID: 27396407
  36. Study demonstrate high concentrations of clusterin (CLU) in mucus samples and significantly reduced CLU levels in cases with endometriosis receiving contraception compared to cases with endometriosis without contraception. PMID: 27071964
  37. Risk-reducing clusterin genotypes protect against the consequences of poor vascular health on executive function performance and change in older adults without dementia. PMID: 27143425
  38. Addition of the minor allele for rs670139 (MS4A4E), rs9331896 (CLU), and rs12155159 (NME8) was nominally associated with change on the DWRT, DSST, and WFT, respectively, in whites. For rs670139 and rs9331896 the association was only significant in individuals bearing at least one APOE 4 allele in stratified analyses. PMID: 27781389
  39. CLU expression is related to the cellular type and inversely correlated with the presence of lymph node metastases in medullary thyroid carcinoma. PMID: 28411179
  40. Melittin is capable of suppressing tumor growth and promoting gemcitabine sensitivity in pancreatic ductal adenocarcinomas by downregulating cholesterol pathway gene CLU. PMID: 28428074
  41. Data show that ELOVL7, SOCS3, ACSL4 and CLU were upregulated while PRKAR1A and ABCG1 were downregulated in the phlegm-dampness group. PMID: 27928700
  42. Both CLU and PLXNA4 have been genetically associated with Alzheimer disease (AD) risk and our data thus provide a direct relationship between two AD risk genes. Our data suggest that increasing the levels of PLXNA4 or targeting CLU-PLXNA4 interactions may have therapeutic value in AD. PMID: 27378688
  43. Although further studies are required to determine how clusterin suppresses non-specific cellular uptake in phagocytes, our data suggest that clusterin plays a key role in the stealth effect of not only pegylated nanoparticles but also non-pegylated nanoparticles. PMID: 27983983
  44. the initial rapid aggregation of LDL(-) is apparently counterbalanced by the stabilizing effects of minor proteins such as apoA-I and apoJ. These results help identify key determinants for LDL aggregation, fusion and coalescence into lipid droplets in vivo. PMID: 27233433
  45. The rs9331888 "GG" genotype in patients and the "CC" genotype in controls were significantly higher in non-4 allele carriers of APOE The haplotype analysis showed the CLU "GCG" haplotype was a risk haplotype. PMID: 27076484
  46. Study explored the common effects of the clusterin (CLU) rs11136000 variant on the default mode network (DMN) in amnestic mild cognitive impairment (aMCI) subjects and remitted geriatric depression (RGD) subjects. The CLU rs11136000 variant could consistently affect the changing of DMN patterns of aMCI and RGD subjects in the frontal cortex. PMID: 28233427
  47. Findings indicate that in Alzheimer's disease, clusterin increases, particularly in regions with most abundant Abeta, but because the increase does not match the rising level of Abeta42, the molar ratio of clusterin : Abeta42 in those regions falls, probably contributing to Abeta deposition within the tissue PMID: 27248362
  48. possible local regulatory role for clusterin in the adipose tissue rather than its systemic involvement in the regulation of energy homeostasis PMID: 27070750
  49. Study identified significant association of the CLU rs9331888 polymorphism with Alzheimer's disease susceptibility in Caucasian population but not in East Asian population (Meta-analysis). PMID: 25633098
  50. Our results suggest clusterin plays a role in modulating the inflammatory response of acute and chronic traumatic brain injury and that it is a useful marker for TBI, particularly in cases with short survival times. Its prominent accumulation in astrocytes, alongside a mounting inflammatory response and activation of microglial cells supports a potential role in the neurodegenerative changes that occur as a result of TBI. PMID: 27365216
  51. Seminal clusterin was a significant predictor of sperm retrieval in infertile men. PMID: 26939938
  52. abnormal sCLU expression associated with tumor progression could be a potential diagnostic and prognostic biomarker for HCC. PMID: 26264614
  53. CLU protein is involved with late onset Alzheimer's disease. [review] PMID: 27773727
  54. When clusterin is expressed in endometrial tumors, it is associated with lower stage. PMID: 27079858
  55. increased levels of clusterin are associated with IUGR and probably predictive for adverse pregnancy outcomes in preeclampsia PMID: 26352072
  56. cross-domain interactions showed that personality trait effects on declarative memory were moderated by APOE and CLU allelic risk. PMID: 26343804
  57. we identified sCLU as a novel miR-378 target, and we showed that targeting sCLU via miR-378 may help disable the chemoresistance against cisplatin in lung adenocarcinoma cells. PMID: 26781643
  58. The genotype frequency of CLU (rs11136000, rs1532278, rs7012010, rs9331888) in a cohort of patients with CAA-associated intracerebral hemorrhage was compared with hypertension-associated ICH, Alzheimer disease, and controls. The CLU genotypes were not different among groups. ApoJ/clusterin appears to be a chaperone related to distinctive lesions in CAA brains. PMID: 26661731
  59. Increased levels of neutrophil defensin 1, apolipoprotein E, clusterin, and zinc-alpha-2-glycoprotein are present in carotid atherosclerotic plaque secretomes. PMID: 26795031
  60. Increased CLU expression is associated with nasopharyngeal carcinoma metastasis. PMID: 26716898
  61. Our results suggest a wider role for clusterin associated with white matter damage in addition to its ability to chaperone proteins for clearance via the perivascular drainage pathways in several disease states. PMID: 25940137
  62. A low prevalence of AD was found in Tibetans from the Qinghai-Tibet plateau and negatively associated with AD in this population with the CLU genotype at rs9331888. PMID: 26757186
  63. The results of the presented "feasibility" study support the role of clusterin in Parkinson disease pathogenesis PMID: 26810527
  64. This review connects the biochemistry and molecular cell biology of CLU with medical aspects, to formulate a hypothesis of a CLU function in health and disease. [review] PMID: 26673020
  65. This study demonstrated that Increased extracellular clusterin in the prefrontal cortex in schizophrenia. PMID: 26482819
  66. Plasma Clusterin glycosylation is associated with hippocampal atrophy in Alzheimer's disease patients. PMID: 26488311
  67. It is an endophenotype for Alzheimer's disease and may play a role in AD by influencing immune system changes that have been observed in AD or by disrupting healing after neurodegeneration. PMID: 26545630
  68. We replicated the rs11136000 (CLU)- rs670139 (MS4A4E) interaction, demonstrated an association in APOE epsilon4 negative subjects, and reported evidence of a possible dominant effect for MS4A4E. PMID: 26449541
  69. The expression of clusterin is significantly correlated with the biological characteristics of ovarian cancer cells, it may be a potential molecular for ovarian cancer treatment PMID: 26293319
  70. L1-mediated induction of CLU, via STAT-1, has an important role in L1-mediated colorectal cancer progression PMID: 26399194
  71. the antioxidant property of clusterin is suggested to regulate the expression of CCL20 in bronchial epithelial cells and the subsequent recruitment of inflammatory dendritic cells in the airway. PMID: 26826245
  72. Clusterin elicits neuroprotective effect by binding to amyloid beta (1-42) oligomers with high affinity and interfering with peptide aggregation. PMID: 26884339
  73. Our data suggest that genetic variants in CLU and TNF-alpha genes do not play a major role in the development of PEXS and PEXG in the South Indian population. PMID: 25849827
  74. Clusterin expression was increased in reactive astrocytes in multiple sclerosis white matter lesions. PMID: 26683597
  75. we show that young individuals with the CLU rs11136000 risk variant (C) have higher activation levels in memory-related prefrontal and limbic areas during a WM task. We also found subtle reductions in gray matter in the right hippocampal formation in carriers of the risk variant. PMID: 25496871
  76. Lpd of P. aeruginosa is a surface exposed moonlighting protein that binds two human terminal pathway inhibitors, vitronectin and clusterin PMID: 26368530
  77. Clusterin was isolated from the pericardial fluid following myocardial infarction. PMID: 26159041
  78. increased apoJ in hyperlipemic serum is paradoxically associated with decreased apoJ content in lipoproteins PMID: 26081122
  79. The pathogenic effect of mutation p.I360N and 10 additional CLU mutations on protein localization and secretion suggest reduced CLU secretion as the mode of action for 3 of them: a frameshift mutation and 2 AA substituions in the disulfide bridge. PMID: 26179372
  80. showed that the presence of these two clusterin glycoforms differed significantly in the plasma of patients prior to and after curative nephrectomy for localized ccRCC. PMID: 25855029
  81. A CLU protein isoform is upregulated in papillary carcinomas. PMID: 25934174
  82. There was no association between CLU polymorphism and Alzheimer disease. PMID: 26738351
  83. sCLU overexpression decreased cisplatin chemosensitivity of lung cancer. PMID: 25884382
  84. The mass spectrometry-based analysis of the global plasma proteome identified CLU as an early biomarker of colorectal cancer, at least in men. PMID: 25884309
  85. PICALM and CLU risk genotypes exert differential impacts on the hippocampal resting-state functional connectivity in healthy young subjects. PMID: 24578178
  86. the rs11136000 of CLU SNP might be related to development of behavioral and psychological symptoms of dementia and Alzheimer's disease in female Turkish subpopulation PMID: 26434199
  87. Clusterin is an independent predictive factor for prognosis of hepatocellular carcinoma and it facilitates metastasis through EIF3I/Akt/MMP13 signaling. PMID: 25609201
  88. fasting plasma clusterin levels correlate with the parameters of adiposity and systemic inflammation in healthy adults. Therefore, the circulating clusterin level may be a surrogate marker for obesity-associated systemic inflammation. PMID: 25076422
  89. These findings suggest that an intracellular form CLU and BIN1 interaction might impact Tau function in neurons and uncover potential new mechanisms underlying the etiology of Tau pathology in in Alzheimer's disease. PMID: 25051234
  90. The results of this study showed significant association between rs2279590 polymorphism and AD in Asian population using additive and recessive models, respectively. We observed no significant heterogeneity between Asian and Caucasian populations. PMID: 24947876
  91. Clusterin facilitates stress-induced lipidation of LC3 and autophagosome biogenesis to enhance cancer cell survival. PMID: 25503391
  92. Its variants are not associated with southern Chinese patients with Alzheimer's disease PMID: 24958191
  93. CLU SNP rs9331888 might be associated with an increased AD risk in Caucasian population, but not in Asian population. PMID: 25703218
  94. High clusterin expression is associated with hepatocellular carcinoma invasion. PMID: 22949882
  95. Clusterin promoter histone H3K9me3 and H3K27me3 marks are elevated in comparison to H3K4me3 and H3K9AcS10P marks in colon cancer cell lines. PMID: 25917404
  96. silencing CLU led to downregulation of beta-catenin (q = 13.544, P = 0.001), suggesting that downregulation of CLU might be a key point to reverse multidrug resistance of HepG2/ADM cells. PMID: 25600802
  97. Clusterin may play a key role during tumorigenesis and tumor progression of esophageal squamous cell carcinoma. PMID: 25574066
  98. High clusterin expression is associated with bladder cancer. PMID: 24696417
  99. ApoJ facilitates infectious HCV particle production via stabilization of core/NS5A, which might surround lipid droplets at the ER-Golgi membrane contact site. PMID: 24996046
  100. we show for the first time that the expression of CLU 2 mRNA is driven by a novel promoter, P2, whose activity responds to epigenetic drugs treatment through changes in histone modifications. PMID: 25464035

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Subcellular Location Isoform 1: Secreted, Note=Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress, SUBCELLULAR LOCATION: Nucleus, Cytoplasm, Mitochondrion membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, cytosol, Microsome, Endoplasmic reticulum, Cytoplasmic vesicle, secretory vesicle, chromaffin granule
Protein Families Clusterin family
Tissue Specificity Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain,
Database Links

HGNC: 2095

OMIM: 185430

KEGG: hsa:1191

STRING: 9606.ENSP00000315130

UniGene: Hs.436657

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