Recombinant Rickettsia conorii Outer membrane protein A (ompA), partial

Code CSB-EP706579RMS
MSDS
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
ompA
Uniprot No.
Research Area
Microbiology
Alternative Names
ompA; RC1273; Outer membrane protein A; 190 kDa antigen; Cell surface antigen; rOmp A; rOmpA) [Cleaved into: 120 kDa surface-exposed protein; 120 kDa outer membrane protein OmpA); 32 kDa beta peptide]
Species
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Source
E.coli
Expression Region
1734-2021aa
Target Protein Sequence
DMDAKFGAWISPFVGNATQKMCNSISGYKSDTTGGTIGFDGFVSDDLVLGLAYTRADTDIKLKNNKTGDKNKVESNIYSLYGLYSVPYENLFVEAIASYSDNKIRSKSRRVIATTLETVGYQTANGKYKSESYTGQLMAGYTYMMSENINLTPLAGLRYSTIKDKSYKETGTTYQNLTVKGKNYNTFDGLLGAKVSSNINVNEIVLTPELYAMVDYAFKNKVSAIDARLQGMTAPLPTNSFKQSKTSFDVGVGVTAKHKMMEYGINYDTNIGSKYFAQQGSVKVRVNF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
51.8kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

Why aa 1734-2021 were selected for expression? What is the function of this region of the protein?

A:
Thanks for your inquiry. The region 1734-2021 corresponds to Autotransporter domain which has been purchased by other customers before.
In molecular biology, an autotransporter domain is a structural domain found in some bacterial outer membrane proteins.
The domain is always located at the C-terminal end of the protein and forms a beta-barrel structure.
The barrel is oriented in the membrane such that the N-terminal portion of the protein, termed the passenger domain, is presented on the cell surface.
These proteins are typically virulence factors, associated with infection or virulence in pathogenic bacteria.
Passenger domains structurally characterized to date have been shown to be dominated by a protein fold known as a beta helix, typified by pertactin.
The folding of this domain is thought to be intrinsically linked to its method of outer membrane translocation.
If you have interest in other expression region, please consult with us and we will evaluate if we can provide your specified expression region.

Target Background

Function
Elicits protective immunity.
Subcellular Location
[Outer membrane protein A]: Periplasm.; [120 kDa surface-exposed protein]: Secreted. Cell surface. Note=Surface exposed. This bacterium is covered by a S-layer with hexagonal symmetry.; [32 kDa beta peptide]: Cell outer membrane; Multi-pass membrane protein. Note=The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane.
Protein Families
Rickettsiae OmpA/OmpB family
Database Links

KEGG: rco:RC1273

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