Human Pancreatic Polypeptide,PP ELISA Kit

Code CSB-E09206h
Size 96T,5×96T,10×96T
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Product Details

Target Name
Pancreatic Polypeptide,PP
Alternative Names
PPY ELISA Kit; PNPPancreatic prohormone ELISA Kit; Pancreatic polypeptide ELISA Kit; PP ELISA Kit; Obinepitide) [Cleaved into: Pancreatic hormone ELISA Kit; PH); Pancreatic icosapeptide ELISA Kit; PI)] ELISA Kit
Abbreviation
PP
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %86
Range %83-89
1:2Average %99
Range %84-102
1:4Average %89
Range %84-93
1:8Average %97
Range %94-100
Recovery
The recovery of human PP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8995-94
EDTA plasma (n=4)108104-112
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.353 2.369 2.361 2.239
10001.470 1.420 1.445 1.323
5000.795 0.763 0.779 0.657
2500.473 0.498 0.486 0.364
1250.315 0.328 0.322 0.200
62.50.232 0.241 0.237 0.115
31.250.171 0.179 0.175 0.053
00.123 0.120 0.122
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human Pancreatic Polypeptide (PP) ELISA Kit was designed for the quantitative measurement of Human Pancreatic Polypeptide (PP) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

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Target Background

Function
(From Uniprot)
Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.; The physiological role for the icosapeptide has not yet been elucidated.
Gene References into Functions
  1. Fasting human pancreatic polypeptide (HPP) levels are similar in chronic pancreatitis (CP) and pancreatic ductal adenocarcinoma (PDAC) and controls regardless of glycemic status. PMID: 29771765
  2. Because of its properties, the PP appears to be a useful marker of the endocrine insufficiency of the pancreas and a specific prognostic parameter of developing diabetes due to chronic pancreatitis PMID: 26766123
  3. Peptide YY and PP are associated with circulating innate pro-inflammatory cytokines in patients after acute pancreatitis and cumulatively contribute to nearly half of the variance of IL-6, MCP-1, and TNFalpha. Future research is warranted to investigate the signaling pathways that underlie these associations. PMID: 27918953
  4. GIP and pancreatic polypeptide plasma concentrations are lower in pancreatic cancer irrespective of the degree of glucose intolerance as compared to Type 2 diabetic patients and healthy controls. PMID: 28027898
  5. Immunohistochemical distribution of this peptide in the epidermal skin (from abdomen, breast and face) of healthy women was analysed. PMID: 26002416
  6. The most important parameter in diagnosis of HG [Hyperemesis gravidarum ]was plasma PP level PMID: 25990478
  7. These data demonstrate glucose-regulated secretion of PP and its effects on glucagon release through PPYR1 receptors expressed by alpha-cells. PMID: 25445712
  8. Pancreatic polypeptide is recognized by two hydrophobic domains of the human Y4 receptor binding pocket. PMID: 24375409
  9. The diagnostic accuracy of the tumor markers CgA, PP, and glucagon for pancreatic neuroendocrine tumors in multiple endocrine neoplasia type 1 is low. PMID: 23956349
  10. These findings underscore the important role of the NPY-Y receptor system at several levels of the gut-brain axis in which NPY, peptide yy and pancreatic polypeptide operate both as neural and endocrine messengers--{REVIEW} PMID: 22979996
  11. Pancreatic polypeptide (and peptide tyrosine-tyrosine) increases in obese woman on caloric restriction following high protein liquid meals compared to high carbohydrate liquid meals. PMID: 23371976
  12. Data suggest that beta-cell function improves in overweight subjects with type 2 diabetes who lose weight by dieting; this change is associated with a decrease in PPY secretion (i.e., plasma PPY is decreased). PMID: 22673566
  13. Self-association of PPY with phospholipid micelles addressed the delivery issues of the peptide for diabetes treatment. PMID: 22399387
  14. Women with bulimia nervosa have significantly lower fasting and postprandial serum concentrations of pancreatic polypeptide (and GLP-1) than control subjects. PMID: 21813805
  15. Distribution of pancreatic polypeptide and peptide YY PMID: 11825640
  16. Pancreatic polypeptide in pancreatitis PMID: 11825647
  17. Pancreatic polypeptide-related tumors PMID: 11825648
  18. Autonomic neuropathy is associated with impaired neuropeptide Y and this peptide responses to insulin-induced hypoglycaemia in Type I diabetic patients. PMID: 12187924
  19. Early postprandial insulin and PP secretory responses were higher in Pima Indians compared with those of Caucasians. PMID: 14988250
  20. Pancreatic polypeptide contributes to the regulation of energy balance in humans. PMID: 15561938
  21. in healthy humans the presence of fat in the small intestine suppresses ghrelin secretion, and fat-induced suppression of ghrelin and stimulation of peptide YY and pancreatic polypeptide is dependent on fat digestion PMID: 15998659
  22. Inhibits gastric emptying of solid food and delays the postprandial rise in plasma glucose and insulin. Is suggested to have physiological role in pancreatic postprandial counterregulation of gastric emptying and insulin secretion. PMID: 15998783
  23. human pancreatic polypeptide inhibits TFF2 secretion in a diurnal rhythm PMID: 16359755
  24. Age and sex may modulate the association between plasma PP level and the intra-abdominal fat area, suggesting that they may be determinants of parasympathetic activity. PMID: 17171555

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Subcellular Location
Secreted.
Protein Families
NPY family
Database Links

HGNC: 9327

OMIM: 167780

KEGG: hsa:5539

STRING: 9606.ENSP00000225992

UniGene: Hs.558368

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