Mouse N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Code CSB-E07444m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse NAG ELISA Kit was designed for the quantitative measurement of Mouse NAG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 μIU/mL-1000 μIU/mL and the sensitivity is 3.9 μIU/mL.

Target Name N-acetyl-β-D-glucosaminidase,NAG
Alternative Names Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
Abbreviation NAG
Uniprot No. P20060
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 μIU/mL-1000 μIU/mL
Sensitivity 3.9 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10 Average % 93  
Range % 85-99  
1:20 Average % 95  
Range % 92-98  
1:40 Average % 90  
Range % 87-100  
1:80 Average % 88  
Range % 86-98  
Recovery
The recovery of mouse NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 86-101  
EDTA plasma (n=4) 95 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average Corrected  
1000 2.555 2.529 2.542 2.398  
500 2.084 2.105 2.095 1.951  
250 1.442 1.467 1.455 1.311  
125 0.946 0.955 0.951 0.807  
62.5 0.413 0.408 0.411 0.267  
31.2 0.321 0.326 0.324 0.180  
15.6 0.244 0.239 0.242 0.098  
0 0.142 0.145 0.144    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
Gene References into Functions
  1. mast cell granule beta-hexosaminidase is crucial for defense against bacterial invasion, but is not involved in the allergic response. the bactericidal mechanism of beta-hexosaminidase involves degradation of bacterial cell wall peptidoglycan. PMID: 25015817
  2. transgenic inducible strains of Sandhoff disease mice provide a dynamic platform with which to explore the pathophysiological sequelae immediately after loss of neuronal lysosomal beta-hexosaminidase activity. PMID: 23028353
  3. Thymic involution and corticosterone level in Sandhoff disease model mice. PMID: 21598013
  4. These data suggest that the restricted ketogenic diet and N-butyldeoxynojirimycin may be a potential combinatorial therapy for Sandhoff disease. PMID: 20374428
  5. Data suggest that pathogenesis of Sandhoff disease (heritable beta-hexosaminidase deficiency) involves an increase in macrophage-inflammatory protein 1alpha that induces monocytes to infiltrate the CNS and trigger neuronal apoptosis. PMID: 15155903
  6. The bicistronic beta-hexosaminidase vector can reverse the biochemical defects and down-stream consequences in Sandhoff neurons, reinforcing its potential for Sandhoff disease in vivo gene therapy. PMID: 16441513
  7. Beta-hexosaminidase is a peptidoglycan hydrolase that surprisingly exerts its mycobactericidal effect at the macrophage plasma membrane during mycobacteria-induced secretion of lysosomes PMID: 18180457
  8. There was no change in the level of GM2 storage and pro-apoptotic activity or astrocyte activation in HexB-/- knockout mice PMID: 18657867

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Subcellular Location Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
Protein Families Glycosyl hydrolase 20 family
Database Links

KEGG: mmu:15212

STRING: 10090.ENSMUSP00000022169

UniGene: Mm.27816

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