Code | CSB-E05070r |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 90 | ||||
Range % | 85-94 | |||||
1:200 | Average % | 95 | ||||
Range % | 91-99 | |||||
1:400 | Average % | 84 | ||||
Range % | 80-89 | |||||
1:800 | Average % | 89 | ||||
Range % | 84-96 |
The recovery of rat INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 90-100 | ||||
EDTA plasma (n=4) | 92 | 88-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The rat Insulin (INS) ELISA kit is a quantitative assay specifically designed for detecting insulin in rat samples. This kit is optimized for rat species and is compatible with sample types such as serum, plasma, and cell culture supernates. The detection range of the kit is from 15.6 nIU/mL to 1000 nIU/mL, with a sensitivity of 3.9 nIU/mL, ensuring accurate measurement even at low concentrations. The assay principle is based on a sandwich method, providing reliable and precise results. The assay can be completed within 1 to 5 hours, making it efficient for research purposes. A small sample volume of 50-100 µl is required for analysis. The detection wavelength for this ELISA kit is 450 nm, allowing for optimal detection of insulin levels in rat samples. This kit has been used in experimental sections of more than 84 literature.
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Seeking for ELISA kits of rat INSULIN.I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.