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Rat Insulin,INS ELISA Kit

Citations (77) Protocol
Code CSB-E05070r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat INS ELISA Kit was designed for the quantitative measurement of Rat INS protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 nIU/mL-1000 nIU/mL and the sensitivity is 3.9 nIU/mL .
Target Name Insulin,INS
Alternative Names
Ins1 ELISA Kit; Ins-1 ELISA Kit; Insulin-1 [Cleaved into: Insulin-1 B chain; Insulin-1 A chain] ELISA Kit
Abbreviation INS
Uniprot No. P01322
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 nIU/mL-1000 nIU/mL
Sensitivity 3.9 nIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:100 Average % 90  
Range % 85-94  
1:200 Average % 95  
Range % 91-99  
1:400 Average % 84  
Range % 80-89  
1:800 Average % 89  
Range % 84-96  
Recovery
The recovery of rat INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-100  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nIU/ml OD1 OD2 Average Corrected  
1000 2.372 2.234 2.303 2.150  
500 1.859 1.812 1.836 1.683  
250 1.429 1.365 1.397 1.244  
125 1.027 1.097 1.062 0.909  
62.5 0.576 0.589 0.583 0.430  
31.2 0.341 0.317 0.329 0.176  
15.6 0.213 0.207 0.210 0.057  
0 0.155 0.151 0.153 　  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Related Products

Ins1 antibodies

Ins1 Proteins

Customer Reviews and Q&A

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■ Q&A
Q:

Seeking for ELISA kits of rat INSULIN.I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.

A:
Thanks for your inquiry.
For the mentiond detection targets,we have the following kit:
#CSB-E05070r,Rat Insulin,INS ELISA Kit
And we use kits to test ordinary rats.While there is not big problems to detect SD Rats.Pls take your actual test results as the real results.

Target Background

Function
(From Uniprot)
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Gene References into Functions
  1. In conclusion, TRPV4 differently regulates insulin mRNA expression in INS-1E cells via ERK1/2 and NO-dependent mechanisms. PMID: 28359774
  2. CART is important for maintaining the beta-cell phenotype. PMID: 28237718
  3. Expression of ghrelin and ghrelin receptor and glucose stimulated insulin secretion (GSIS) of pancreatic islets. PMID: 28410130
  4. glucagon plus insulin increases FGF21 transcription by stimulating ATF4 expression PMID: 28188284
  5. Under insulin stimulation, and in spite of the less activated mTOR function in high altitude rats, low altitude rats had higher glycogen content and reduced levels of gluconeogenic enzymes with a more enhanced insulin signaling, mainly due to higher levels of phosphorylated IRS1. PMID: 28084108
  6. Reduced sortilin levels associate with lower VLDL apo B secretion. Sortilin facilitates VLDL-B100 secretion by insulin sensitive McArdle RH7777 cells. PMID: 27495870
  7. Data show that quercetin acutely stimulates insulin release, inhibits cell proliferation and induces apoptosis of INS-1 beta cells. PMID: 27336168
  8. Insulin deficiency increases the expression of IGF-1 and IGF-1R in renal mesangial cells and the kidney of diabetic rats, which contributes to the development of diabetic nephropathy. PMID: 26775660
  9. Data suggest that rosuvastatin has the potential to increase basal insulin secretion and reduce glucose-induced insulin secretion. PMID: 26986474
  10. These results identify a novel role for Jagn1 in regulating proinsulin biosynthesis. PMID: 26882284
  11. Data suggest glucose-induced insulin secretion is first up-regulated (acute infection), then down-regulated (chronic infection) in beta cells infected with S. aureus; Pla2g4a is up-regulated in acute infection; Pla2g6 is up-regulated in chronic infection. PMID: 26632509
  12. These observations support the hypothesis that glucagon acts via adenylate cyclase to decrease hepatic GSH levels and induce insulin resistance. PMID: 25961284
  13. Gamma conglutin and glibenclamide increased Ins-1 gene expression in diabetic rats. PMID: 24894193
  14. Data suggest neural mechanism of allostatic insulin secretion in male rats fed liquid diet as meals; allostasis leads to postprandial insulin secretion by pancreatic islets suggestive of neural/neurosecretory regulation to prevent hyperglycemia. PMID: 25159330
  15. Maximal upregulation of many unfolded protein response genes in response to mutant proinsulin production required IRE1. PMID: 25011481
  16. Data indicate that, gestational Di-(2-ethylhexyl)phthalate (DEHP) exposure predisposes F1 offspring to glucometabolic dysfunction by down-regulating the expression of critical genes involved in the insulin signalling pathway. PMID: 25232145
  17. analysis of organization of the beta-structure of rat insulin reveals key regions essential for the propagation and cytotoxicity of amyloid fibrils PMID: 24569992
  18. Data suggest that responsiveness of pancreatic beta cells to glucose level resulting in secretion of insulin involves Glp1/Glp1r (glucagon-like peptide-1/glucagon-like peptide-1 receptor) signaling and GLP1/Glp1r agonists act as hypoglycemic agents. PMID: 24425760
  19. Data indicate that miR-183 was decreased and Foxo1 was upregulated during conversion of dental pulp cells (DPCs) to insulin-producing cells. PMID: 24440707
  20. miR-25 and miR-92a are found as direct modulators of insulin expression; overexpression of miR-25 or miR-92a reduced insulin expression;inhibition of miR-25 and miR-92a expression promoted insulin expression and ultimately enhanced glucose-induced insulin secretion PMID: 24084692
  21. B-cell translocation gene 2 (BTG2) is a crucial regulator in GLP-1-induced insulin gene expression and insulin secretion via upregulation of pancreatic duodenal homeobox-1 (PDX-1) in pancreatic beta-cells. PMID: 23703573
  22. investigation of role of microtubules/actin cytoskeleton in insulin-stimulated glucose uptake in skeletal muscle: Data suggest sequence of reorganization of microtubules, actin remodeling, translocation of GLUT4, and glucose uptake. PMID: 22996137
  23. Exogenous and endogenous ghrelin counteracts GLP-1 action to stimulate cAMP signaling and insulin secretion in islet beta-cells PMID: 22750144
  24. adiponectin in the serum from CR animals increases NO* signaling by activating the insulin pathway PMID: 22319612
  25. Insulin entry into muscle involves a saturable process in the vascular endothelium PMID: 22002008
  26. results revealed a novel function for brain insulin in CTA and an additional source of local synthesis, the central and/or basolateral nuclei of the amygdala. PMID: 22119580
  27. Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) has a role in insulin hypersecretion PMID: 22065581
  28. Estrogen-related receptor gamma (ERRgamma) is a novel transcriptional regulator of phosphatidic acid phosphatase, LIPIN1, and inhibits hepatic insulin signaling PMID: 21911493
  29. NAD(P)H oxidase is an important source of superoxide in pancreatic islets and the activity of NAD(P)H oxidase is involved in the control of insulin secretion by palmitate. PMID: 20857410
  30. Insulin increased plasma membrane GLUT4 and GLUT1 content 30 and 40 min after treatment and CD36 content in plasma membrane fraction. PMID: 21773965
  31. Insulin (Ins) release is altered by anesthetics. PMID: 18165568
  32. insulin stimulates cell proliferation and c-Myc expression in colon cancer cell lines HT29 and Caco-2, intestinal non-cancer cell line IEC-6, and primary fetal rat intestinal cell (FRIC) cultures. PMID: 17993259
  33. the stimulation of glucose uptake in response to insulin or metabolic stress is dependent in cardiomyocytes on the presence of intact microtubules. PMID: 18063688
  34. Insulin down-regulates specific activity of ATP-binding cassette transporter A1 for high density lipoprotein biogenesis through its specific phosphorylation PMID: 21402379
  35. C-peptide may contribute to plaque development and restenosis formation in patients with insulin resistance and early type 2 diabetes mellitus. (C-peptide) PMID: 17068290
  36. AS160 phosphotyrosine-binding domain constructs inhibit insulin-stimulated GLUT4 vesicle fusion with the plasma membrane PMID: 21454690
  37. Data support the hypothesis that brain insulin is required to support optimal baroreflex function and that a decrease in brain insulin contributes to the fall in baroreflex gain during pregnancy. PMID: 21149828
  38. beta2-Syntrophin is a Cdk5 substrate that restrains the motility of insulin secretory granules. PMID: 20886068
  39. Intrauterine growth retardation leads to the functional change of insulin secretion in the newborn. PMID: 20225168
  40. This study identified second messenger systems in neuropeptide Y neurons of arcuate nucleus which regulate neuron activity and physiological processes such as the control of food intake and body weight, under the combined control of insulin and leptin. PMID: 19671839
  41. Leptin and insulin induce mutual resistance for nitric oxide synthase III activation in adipocytes PMID: 19728324
  42. induction of c-myc expression suppresses gene transcription PMID: 11799123
  43. Insulin preincubation effects on rat vessel contractile responses: role of the endothelium cycooxygenase and nitric oxide synthase pathways. PMID: 11824479
  44. mechanisms involved in the insulin-induced acceleration of ET1 contractions; role of the sarcoplasmic reticulum and calcium. PMID: 11824480
  45. pancreatic release of insulin inhibited by ghrelin PMID: 11834435
  46. effects of insulin and the degree of muscle loading on the phosphorylation of Ser(2448), a site in the mammalian target of rapamycin (mTOR) phosphorylated by protein kinase B (PKB) in vitro PMID: 11884412
  47. Glucagon-like peptide-1 analogue LY315902: effect on intestinal motility and release of insulin and somatostatin PMID: 12047915
  48. endogenously released insulin plays an important role in potentiating pancreatic secretion stimulated by exogenous cholecystokinin (CCK) and intraduodenal infusion of casein PMID: 12120208
  49. the signalling pathways and the effects of insulin were compared to those of glutamine and leucine in isolated hepatocytes from normal and streptozotocin-diabetic rats PMID: 12153571
  50. Insulin modulates GLUT4 trafficking at multiple steps, including not only the endocytotic and exocytotic rates, but also the two rate coefficients coupling the three intracellular compartments. PMID: 12450403

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Subcellular Location Secreted.
Protein Families Insulin family
Database Links

KEGG: rno:24505

STRING: 10116.ENSRNOP00000016052

UniGene: Rn.962
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