Rat Insulin,INS ELISA Kit

Instructions
Code CSB-E05070r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Insulin,INS
Alternative Names Ins1 ELISA Kit; Ins-1 ELISA Kit; Insulin-1 [Cleaved into: Insulin-1 B chain; Insulin-1 A chain] ELISA Kit
Abbreviation INS
Uniprot No. P01322
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 nIU/mL-1000 nIU/mL
Sensitivity 3.9 nIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 85-94  
1:200 Average % 95  
Range % 91-99  
1:400 Average % 84  
Range % 80-89  
1:800 Average % 89  
Range % 84-96  
Recovery
The recovery of rat INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-100  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nIU/ml OD1 OD2 Average Corrected  
1000 2.372 2.234 2.303 2.150  
500 1.859 1.812 1.836 1.683  
250 1.429 1.365 1.397 1.244  
125 1.027 1.097 1.062 0.909  
62.5 0.576 0.589 0.583 0.430  
31.2 0.341 0.317 0.329 0.176  
15.6 0.213 0.207 0.210 0.057  
0 0.155 0.151 0.153    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Q&A
Q:

Seeking for ELISA kits of rat INSULIN.I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.

A:
Thanks for your inquiry.
For the mentiond detection targets,we have the following kit:
#CSB-E05070r,Rat Insulin,INS ELISA Kit
And we use kits to test ordinary rats.While there is not big problems to detect SD Rats.Pls take your actual test results as the real results.

Target Data

Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Gene References into Functions
  1. In conclusion, TRPV4 differently regulates insulin mRNA expression in INS-1E cells via ERK1/2 and NO-dependent mechanisms. PMID: 28359774
  2. CART is important for maintaining the beta-cell phenotype. PMID: 28237718
  3. Expression of ghrelin and ghrelin receptor and glucose stimulated insulin secretion (GSIS) of pancreatic islets. PMID: 28410130
  4. glucagon plus insulin increases FGF21 transcription by stimulating ATF4 expression PMID: 28188284
  5. Under insulin stimulation, and in spite of the less activated mTOR function in high altitude rats, low altitude rats had higher glycogen content and reduced levels of gluconeogenic enzymes with a more enhanced insulin signaling, mainly due to higher levels of phosphorylated IRS1. PMID: 28084108
  6. Reduced sortilin levels associate with lower VLDL apo B secretion. Sortilin facilitates VLDL-B100 secretion by insulin sensitive McArdle RH7777 cells. PMID: 27495870
  7. Data show that quercetin acutely stimulates insulin release, inhibits cell proliferation and induces apoptosis of INS-1 beta cells. PMID: 27336168
  8. Insulin deficiency increases the expression of IGF-1 and IGF-1R in renal mesangial cells and the kidney of diabetic rats, which contributes to the development of diabetic nephropathy. PMID: 26775660
  9. Data suggest that rosuvastatin has the potential to increase basal insulin secretion and reduce glucose-induced insulin secretion. PMID: 26986474
  10. These results identify a novel role for Jagn1 in regulating proinsulin biosynthesis. PMID: 26882284
  11. Data suggest glucose-induced insulin secretion is first up-regulated (acute infection), then down-regulated (chronic infection) in beta cells infected with S. aureus; Pla2g4a is up-regulated in acute infection; Pla2g6 is up-regulated in chronic infection. PMID: 26632509
  12. These observations support the hypothesis that glucagon acts via adenylate cyclase to decrease hepatic GSH levels and induce insulin resistance. PMID: 25961284
  13. Gamma conglutin and glibenclamide increased Ins-1 gene expression in diabetic rats. PMID: 24894193
  14. Data suggest neural mechanism of allostatic insulin secretion in male rats fed liquid diet as meals; allostasis leads to postprandial insulin secretion by pancreatic islets suggestive of neural/neurosecretory regulation to prevent hyperglycemia. PMID: 25159330
  15. Maximal upregulation of many unfolded protein response genes in response to mutant proinsulin production required IRE1. PMID: 25011481
  16. Data indicate that, gestational Di-(2-ethylhexyl)phthalate (DEHP) exposure predisposes F1 offspring to glucometabolic dysfunction by down-regulating the expression of critical genes involved in the insulin signalling pathway. PMID: 25232145
  17. analysis of organization of the beta-structure of rat insulin reveals key regions essential for the propagation and cytotoxicity of amyloid fibrils PMID: 24569992
  18. Data suggest that responsiveness of pancreatic beta cells to glucose level resulting in secretion of insulin involves Glp1/Glp1r (glucagon-like peptide-1/glucagon-like peptide-1 receptor) signaling and GLP1/Glp1r agonists act as hypoglycemic agents. PMID: 24425760
  19. Data indicate that miR-183 was decreased and Foxo1 was upregulated during conversion of dental pulp cells (DPCs) to insulin-producing cells. PMID: 24440707
  20. miR-25 and miR-92a are found as direct modulators of insulin expression; overexpression of miR-25 or miR-92a reduced insulin expression;inhibition of miR-25 and miR-92a expression promoted insulin expression and ultimately enhanced glucose-induced insulin secretion PMID: 24084692
  21. B-cell translocation gene 2 (BTG2) is a crucial regulator in GLP-1-induced insulin gene expression and insulin secretion via upregulation of pancreatic duodenal homeobox-1 (PDX-1) in pancreatic beta-cells. PMID: 23703573
  22. investigation of role of microtubules/actin cytoskeleton in insulin-stimulated glucose uptake in skeletal muscle: Data suggest sequence of reorganization of microtubules, actin remodeling, translocation of GLUT4, and glucose uptake. PMID: 22996137
  23. Exogenous and endogenous ghrelin counteracts GLP-1 action to stimulate cAMP signaling and insulin secretion in islet beta-cells PMID: 22750144
  24. adiponectin in the serum from CR animals increases NO* signaling by activating the insulin pathway PMID: 22319612
  25. Insulin entry into muscle involves a saturable process in the vascular endothelium PMID: 22002008
  26. results revealed a novel function for brain insulin in CTA and an additional source of local synthesis, the central and/or basolateral nuclei of the amygdala. PMID: 22119580
  27. Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) has a role in insulin hypersecretion PMID: 22065581
  28. Estrogen-related receptor gamma (ERRgamma) is a novel transcriptional regulator of phosphatidic acid phosphatase, LIPIN1, and inhibits hepatic insulin signaling PMID: 21911493
  29. NAD(P)H oxidase is an important source of superoxide in pancreatic islets and the activity of NAD(P)H oxidase is involved in the control of insulin secretion by palmitate. PMID: 20857410
  30. Insulin increased plasma membrane GLUT4 and GLUT1 content 30 and 40 min after treatment and CD36 content in plasma membrane fraction. PMID: 21773965
  31. Insulin (Ins) release is altered by anesthetics. PMID: 18165568
  32. insulin stimulates cell proliferation and c-Myc expression in colon cancer cell lines HT29 and Caco-2, intestinal non-cancer cell line IEC-6, and primary fetal rat intestinal cell (FRIC) cultures. PMID: 17993259
  33. the stimulation of glucose uptake in response to insulin or metabolic stress is dependent in cardiomyocytes on the presence of intact microtubules. PMID: 18063688
  34. Insulin down-regulates specific activity of ATP-binding cassette transporter A1 for high density lipoprotein biogenesis through its specific phosphorylation PMID: 21402379
  35. C-peptide may contribute to plaque development and restenosis formation in patients with insulin resistance and early type 2 diabetes mellitus. (C-peptide) PMID: 17068290
  36. AS160 phosphotyrosine-binding domain constructs inhibit insulin-stimulated GLUT4 vesicle fusion with the plasma membrane PMID: 21454690
  37. Data support the hypothesis that brain insulin is required to support optimal baroreflex function and that a decrease in brain insulin contributes to the fall in baroreflex gain during pregnancy. PMID: 21149828
  38. beta2-Syntrophin is a Cdk5 substrate that restrains the motility of insulin secretory granules. PMID: 20886068
  39. Intrauterine growth retardation leads to the functional change of insulin secretion in the newborn. PMID: 20225168
  40. This study identified second messenger systems in neuropeptide Y neurons of arcuate nucleus which regulate neuron activity and physiological processes such as the control of food intake and body weight, under the combined control of insulin and leptin. PMID: 19671839
  41. Leptin and insulin induce mutual resistance for nitric oxide synthase III activation in adipocytes PMID: 19728324
  42. induction of c-myc expression suppresses gene transcription PMID: 11799123
  43. Insulin preincubation effects on rat vessel contractile responses: role of the endothelium cycooxygenase and nitric oxide synthase pathways. PMID: 11824479
  44. mechanisms involved in the insulin-induced acceleration of ET1 contractions; role of the sarcoplasmic reticulum and calcium. PMID: 11824480
  45. pancreatic release of insulin inhibited by ghrelin PMID: 11834435
  46. effects of insulin and the degree of muscle loading on the phosphorylation of Ser(2448), a site in the mammalian target of rapamycin (mTOR) phosphorylated by protein kinase B (PKB) in vitro PMID: 11884412
  47. Glucagon-like peptide-1 analogue LY315902: effect on intestinal motility and release of insulin and somatostatin PMID: 12047915
  48. endogenously released insulin plays an important role in potentiating pancreatic secretion stimulated by exogenous cholecystokinin (CCK) and intraduodenal infusion of casein PMID: 12120208
  49. the signalling pathways and the effects of insulin were compared to those of glutamine and leucine in isolated hepatocytes from normal and streptozotocin-diabetic rats PMID: 12153571
  50. Insulin modulates GLUT4 trafficking at multiple steps, including not only the endocytotic and exocytotic rates, but also the two rate coefficients coupling the three intracellular compartments. PMID: 12450403
  51. prolonged exposure to elevated levels of palmitate affects glucose-stimulated insulin gene expression via transcriptional mechanisms and ceramide synthesis PMID: 12771145
  52. insulin is capable of increasing the abundance and functional activity of GLUT5 in skeletal muscle cells, most likely mediated via activation of the GLUT5 promoter PMID: 12914929
  53. long term nutritional state sets the preproinsulin mRNA level in the beta-cell at which translation control regulates short term changes in rates of proinsulin biosynthesis in response to glucose; this is not mediated by any autocrine effect of insulin. PMID: 12928442
  54. Resistance training increases insulin-stimulated carbohydrate metabolism in normal skeletal muscle and reverses high-fat diet-induced skeletal muscle insulin resistance by altering both insulin signaling cascade and glucose transporter effector system. PMID: 14707149
  55. Expression of p35 and CDK5 in insulin-producing beta-cellsis new signaling pathway controlled by glucose, and its functional role may comprise regulation of various biological processes in beta-cells, such as expression of the insulin gene. PMID: 14976144
  56. Improvements of insulin action in insulin-resistant skeletal muscle after exercise training were associated with increases in IRS-1 protein and IRS-1 associated with p85. PMID: 15068957
  57. GIP, GLP-1, insulin and gastrin have roles in ghrelin release in the isolated rat stomach PMID: 15093702
  58. CaM-KK/CaM-KIV cascade might play an important role in glucose-upregulated transcriptional activation of the insulin gene PMID: 15161751
  59. Insulin can negatively control pancreatic somatostatin mRNA and hormone content and positively control CCK(B)R mRNA; the CCK(B)R protein appears to be delayed. PMID: 15161757
  60. insulin and PDGF rely differently on the actin cytoskeleton and on tetanus-toxin-sensitive VAMPs for mobilizing GLUT4 PMID: 15466888
  61. Insulin has a role in increasing hypothalamo-pituitary-adrenal activity, while diabetes lowers peak stress responses PMID: 15564337
  62. increased hepatocyte CYP2E1 expression and the presence of steatohepatitis result in the down-regulation of insulin signaling PMID: 15632182
  63. full induction of the mature and transcriptionally active form of SREBP-1c protein requires insulin PMID: 15637161
  64. data showed that the effects of high free fatty acids (FFAs) levels on plasma leptin were mediated by the rise of insulin concentration PMID: 15687408
  65. Insulin protectes theca-interstitial cell cells from apoptosis, reducing DNA fragmentation. PMID: 15833775
  66. results indicate that the ERK1/2 pathway modulates partners of NFAT, which may either stimulate or repress insulin gene transcription during stimulatory and chronic exposure to elevated glucose PMID: 15899886
  67. ATP (purinergic receptor antagonists) signals entrain the islets into a common rhythm resulting in pulsatile release of insulin. PMID: 15983214
  68. NADPH/glutaredoxin/thioredoxin redox regulation mediates a novel signaling pathway of nutrient-induced insulin secretion. PMID: 15983215
  69. The study indicates that hGH stimulates rise in Ca(2+) and insulin secretion mainly through activation of the prolactin receptor and JAK2 and Src kinases in rat insulin-secreting cells PMID: 16597920
  70. insulin-regulated resensitization of GH signaling may be necessary to reset the complete response to GH after a normal, physiologic pulse of GH PMID: 16717097
  71. Ins induces tyr-phosphorylation of IRS-4, an effect modulated by Ang II PMID: 16933034
  72. decrease in DIO rat hypothalamic leptin and insulin signaling; high energy diet intake reduces hypothalamic melanocortin and hippocampal insulin binding PMID: 17023527
  73. Suggest an overexpression of CD36 in vascular smooth muscle from diabetic rats and impaired control by insulin. PMID: 17127041
  74. Ins1 signaling pathway modulates nitric oxide synthesis in glucose-inhibited neurons in the ventromedial hypothalamus. PMID: 17170237
  75. The results suggest that neuropeptide W (NPW) but not neuropeptide B (NPB) exerts a potent suppressive effect on blood leptin concentrations in the rat, and this mechanism may be involved in NPW regulation of energy homeostasis. PMID: 17273787
  76. New insulin producing cells can be regenerated from diabetic pancreas by auto-transplanting pancreatic fragments into the activated omentum, an environment rich in growth factors and progenitor cells. PMID: 17292859
  77. The response of adrenomedullin to insulin via insulin responsive genetic elements is reported. PMID: 17327422
  78. Though stage of development significantly affects the levels of peripheral metabolic hormones such as insulin, T4 and T3, acute stress does not appreciably influence their secretion before or after puberty. PMID: 17454971
  79. Our study indicates that palmitate-induced insulin resistance is provoked by two distinct mechanisms. PMID: 17550999
  80. The ATF6-induced suppression of insulin gene expression was associated with up-regulation of SHP gene expression. PMID: 18450959
  81. Insulin depletion and hyperglycemia do not regulate the expression of GLUT8 in testes. PMID: 19194835
  82. acute actions of insulin to augment the sympathoadrenal response to hypoglycemia are likely mediated via mechanisms exterior to the central nervous syst PMID: 19375131
  83. untreated diabetic rats had higher expressions of sterol regulatory element-binding protein 1c, tumor necrosis factor alpha, and Tyr(705) phosphorylation of stat3 levels, which all were down-regulated after insulin treatment PMID: 19375767
  84. Reducing SirT1, by using an antisense oligonucleotide (ASO), decreases fasting hyperglycemia in a rat model of type 2 diabetes mellitus. PMID: 19549853

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Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: rno:24505

STRING: 10116.ENSRNOP00000016052

UniGene: Rn.962

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