Rat Insulin,INS ELISA Kit

Code CSB-E05070r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01322
Lead Time 3-5 working days
Abbreviation INS
Target Name Insulin,INS
Alias N/A
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 nIU/mL-1000 nIU/mL
Sensitivity 3.9 nIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 85-94  
1:200 Average % 95  
Range % 91-99  
1:400 Average % 84  
Range % 80-89  
1:800 Average % 89  
Range % 84-96  
Recovery
The recovery of rat INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-100  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nIU/ml OD1 OD2 Average Corrected  
1000 2.372 2.234 2.303 2.150  
500 1.859 1.812 1.836 1.683  
250 1.429 1.365 1.397 1.244  
125 1.027 1.097 1.062 0.909  
62.5 0.576 0.589 0.583 0.430  
31.2 0.341 0.317 0.329 0.176  
15.6 0.213 0.207 0.210 0.057  
0 0.155 0.151 0.153    
References
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Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: rno:24505

STRING: 10116.ENSRNOP00000016052

UniGene: Rn.962

Q&A and Customer Reviews

Q&A
Q:

Seeking for ELISA kits of rat INSULIN.I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.

A:
Thanks for your inquiry.
For the mentiond detection targets,we have the following kit:
#CSB-E05070r,Rat Insulin,INS ELISA Kit
And we use kits to test ordinary rats.While there is not big problems to detect SD Rats.Pls take your actual test results as the real results.
Customer Reviews

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