Chicken Insulin ELISA kit

Code CSB-E13293C
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
insulin
Alternative Names
INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
Abbreviation
INS
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 μIU/mL-50 μIU/mL
Sensitivity
0.59 μIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken insulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 87-98  
1:2 Average % 96  
Range % 90-101  
1:4 Average % 98  
Range % 95-103  
1:8 Average % 90  
Range % 85-96  
Recovery
The recovery of chicken insulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-100  
EDTA plasma (n=4) 97 95-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average    
50 0.141 0.145 0.143    
25 0.310 0.313 0.312    
12.5 0.685 0.676 0.681    
6.25 1.477 1.472 1.475    
3.12 2.709 2.707 2.708    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Chicken INS ELISA Kit was designed for the quantitative measurement of Chicken INS protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 3.12 μIU/mL-50 μIU/mL and the sensitivity is 0.59 μIU/mL.

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 Q&A
Q:

Regarding your ELISA kit CSB-E13293C, could you please advise on the following:
1) Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Does the standard include additive agent such as antiseptic or preservative? If it includes, what is the composition of said additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry.
CSB-E13293C
Standard:293 cell-derived recombinant protein.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
The kit doesn't contain hazardous substance such as azide, thimerosal, 2-ME, etc.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Gene References into Functions
  1. Data suggest that insulin signaling in skeletal muscle can be regulated by dietary factors (here, dietary content of omega-3 fatty acids). PMID: 24094087
  2. Polymorphisms within the INS gene are associated with 55-week body weight. PMID: 16734694
  3. The present findings suggest the involvement of insulin in neuronal/glial coupling in the cerebellum. PMID: 19135128
Subcellular Location
Secreted.
Protein Families
Insulin family
Database Links
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