Recombinant Duck hepatitis B virus Protein P(P) ,partial

Code CSB-YP322912DHF
Size Pls inquire other sizes
Source Yeast
Code CSB-EP322912DHF
Size Pls inquire other sizes
Source E.coli
Code CSB-EP322912DHF-B
Size Pls inquire other sizes
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP322912DHF
Size Pls inquire other sizes
Source Baculovirus
Code CSB-MP322912DHF
Size Pls inquire other sizes
Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names P
Uniprot No. P17192
Species Duck hepatitis B virus (isolate brown Shanghai duck S5) (DHBV)
Expression Region 1-788
Target Protein Sequence MPQPLKQSLD QSRWLREAEK HLRELENLVD SNLEEEKLKP QLSMGEDVQS PGIGEPLHPN VRAPLSHVVR AATIDLPRLG NKLPAKHHLG KLSGLYQMKG CTFNPEWKVP DISDTHFDLQ VINECPSRNW KYLTPAKFWP KSISYFPVQA GVKAKYPDNV MQHEAIVGKY LNRLYEAGIL YKRISKHLVT FKGKPYNWEL QYLVKQHQVP DGTTTSKING RAENRRRRAP AKSISRPHDS ERDCNMVGQI SNNRSSIRPC ANNGGGKHYA TTRRLACWGG KTIGTDQSYS SRDTSATVDS RGRSESSRGF STISGRKATG NHHHCSNVTN SVETTTRGRS TPGKQVVTRD SSALPESRAS RACHKDSSPQ KEENAWYLRG NTSWPNRITG KLFLVDKNSR NTTEARLVVD FSQFSKGKNA MRFPRYWSPN LSTLRRILPV GMPRISLDLS QAFYHLPLNP ASSSRLAVSD GQHVYYFRKA PMGVGLSPFL LHLFTTALGS EIARRFNIWT FTYMDDFLLC HPNARHLNSI SHAVCSFLQE LGIRINFDKT TPSPVNDIRF LGYQIDQKFM KIEESRWKEL RTVIKKIKIG AWYDWKCIQR FVGHLNFVLP FTKGNIEMLK PMYAAITNKV NFSFSSAYRT LLYKLTMGVC KLAIRPKSSV PLPRVATDAT PTHGAISHIT GGSAVFAFSK VRDIHIQELL MVCLAKIMIK PRCILSDSTF VCHKRYQTLP WHFAMLAKQL LSPIQLYFVP SKYNPADGPS RHKPPDWTAL TYTPLSKAIY IPHRLCGT
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
Still Have Questions? Start a Chat

Target Data

Function Multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated together with the P protein, and reverse-transcribed inside the nucleocapsid. Initiation of reverse-transcription occurs first by binding the epsilon loop on the pgRNA genome, and is initiated by protein priming, thereby the 5'-end of (-)DNA is covalently linked to P protein. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA). The activity of P protein does not seem to be necessary for cccDNA generation, and is presumably released from (+)DNA by host nuclear DNA repair machinery (By similarity).
Protein Families Hepadnaviridae P protein family

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.