Product Details

Purity

>85% (SDS-PAGE)

Target Names

Uniprot No.

Q9Q0L6

Alternative Names

NS; Non-structural protein 1; NS1; NS1A

Species

Influenza A virus (strain A/Goose/Guangdong/1/1996 H5N1 genotype Gs/Gd)

Expression Region

1-230

Target Protein Sequence

MDSNTITSFQ VDCYLWHIRK LLSMRDMCDA PFDDRLRRDQ KALKGRGSTL GLDLRVATME GKKIVEDILK SETNENLKIA IASSPAPRYI TDMSIEEMSR EWYMLMPRQK ITGGLMVKMD QAIMDKRIIL KANFSVLFDQ LETLVSLRAF TESGAIVAEI FPIPSVPGHF TEDVKNAIGI LIGGLEWNDN SIRASENIQR FAWGIHDENG GPSLPPKQKR YMAKRVESEV

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA.; Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in the host nucleus and are no longer exported to the cytoplasm. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.

Gene References into Functions

study demonstrated that the amino acid at positions 55, 66, and 133 of NS1 play an important role in the ability of H5N1 influenza viruses to dampen the host IFN response PMID: 29677653
ANXA2 interacts with NS1 and ANXA2 expression increases highly pathogenic H5N1 avian influenza virus replication. PMID: 28893180
Studies showed that the replacement of the 42nd residue of NS1 has a direct impact on the binding to dsRNA, so that the 42nd residue should be responsible for the high pathogenicity of H5N1. The structural models also suggested that the replacement causes structural change within the RNA-binding domain , leading to the change in the dsRNA-binding affinity. PMID: 26845765
analysis yielded chicken gga-miR-1658* as the potential miRNA which is targeting the NS1 gene of H5N1 genome PMID: 25963812
D120 and R195 of H5N1 influenza virus NS1 were crucial for the binding to host NOLC1. PMID: 25645906
This is the first report to elucidate the novel function of NS1--binding protein collaborating with viral protein NS1 in modulation of host cell physiology. In addition, an alternative role of adaptor protein CRKL in association with NS1 and NS1-BP during influenza A virus infection is demonstrated. PMID: 24220336
H5N1 viral NS1 interacts with host NOLC1 protein. PMID: 23188192
The PDZ and p85beta-binding domains of the NS1 protein are important for the virulence of H5N1 influenza A virus. PMID: 23408626
Findings indicate that the heterologous interactions between non-structural protein 1 (NS1) proteins from different influenza A virus subtypes/strains may be a common event during co-infection. PMID: 22744180
The high pathogenic strains of the avian influenza H5N1 virus isolated in Kazakhstan have NS of different genotypes. The influenza virus strains isolated in 2005 is of NS1E Qinghai genotype. PMID: 21338659
NS1 of H5N1 was an effective inhibitor of cellular pre-mRNA polyadenylation in A549 cells, unlike NS1 of H5N2 and H1N1/2009 PMID: 21163951
Data suggest that NS1 can directly interfere with IFN signaling to enhance viral replication. PMID: 21085662
Virulence determinants of avian H5N1 influenza A virus in mammalian and avian hosts: role of the C-terminal ESEV motif in the viral NS1 protein. PMID: 20686040
found that the D92E mutation eliminated NS1's repressive effect on IFN induction, while the 5-aa deletion resulted in enhanced resistance to TNFalpha responses. PMID: 20228833

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Subcellular Location

Host nucleus. Host cytoplasm.

Protein Families

Influenza A viruses NS1 family

Database Links

KEGG: vg:8656648

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Recombinant Influenza A virus Non-structural protein 1 (NS)

Product Details

Customer Reviews and Q&A

Target Background

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