Recombinant Sendai virus Matrix protein (M)

Code CSB-YP525216SYH
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP525216SYH
MSDS
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Source E.coli
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Code CSB-EP525216SYH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP525216SYH
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP525216SYH
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
M
Uniprot No.
Alternative Names
MMatrix protein; M protein
Species
Sendai virus (strain Ohita) (SeV)
Expression Region
1-348
Target Protein Sequence
MADIYRFPKF SYEDNGTVEP LPLRTGPDKK AIPHIRIVKV GDPPKHGVRY LDLLLLGFFE TPKQTASLGS VSDLTEHTGY SICGSGSLPI GVAKYHGSDQ ELLKACTDLR ITVRRTVRAG EMIVYMVDSI GAPLLPWSGR LRQGMIFNAN KVALAPQCLP VDKDIRFRVV FVNGTSLGAI TIAKIPKTLA DLALPNSISV NLLVTLKTGI STEQKGVLPV LDDQGEKKLN FMVHLGLIRR KVGKIYSVEY CKSKIERMRL IFSLGLIGGI SFHVQVTGTL SKTFMGQLAW KRAVCFPLMD VNPHMNLVIW AASVEITDVD AVFQPAIPRD FRYYPNVVAK NIGRIRKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a crucial role in virion assembly and budding. Forms a shell at the inner face of the plasma membrane and concentrates the HN and F glycoproteins. Acts as a negative regulator for transcription and replication by sticking to the nucleocapsid. This effect might be regulated by the cytoplasmic interaction with tubulin that dissociates the M protein from the nucleocapsid.
Gene References into Functions
  1. Incorporation of the matrix protein in virus particle is required for its function in particle production. PMID: 25113904
  2. The results indicate YLDL motif in M protein is essential for efficient budding in the context of virus infection and suggest involvement of Alix/AIP1 in SeV budding. PMID: 20605035
  3. A YLDL sequence within the M protein showed L-domain activity, and its specific interaction with the N terminus of Alix/AIP1(1-211) was important for the budding of virus-like particles (VLPs) of M protein. PMID: 17166905
Subcellular Location
Virion. Host cytoplasm. Host cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Morbillivirus/respirovirus/rubulavirus M protein family
Database Links

KEGG: vg:1489778

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