The human insulin/INS ELISA kit is used to detect and quantify the concentrations of INS in serum, plasma, cell culture supernates, and tissue homogenates. This kit exclusively recognizes human INS proteins. It adopts a sandwich ELISA technique in which an INS antibody/INS/biotinylated INS complex is formed and develops color reaction after the addition of TMB substrate solution. The intensity of the color is positively relevant to the levels of INS in the sample. This ELISA kit has been confirmed to have high sensitivity, strong specificity, premium precision, and lot-to-lot consistency. See the instructions for more details.
Insulin/INS is the predominant anabolic hormone, promoting carbon energy deposition in the body. Its secretion from beta cells is induced by glucose and Glucagon-like-peptide 1 (GLP-1). It is a critical regulator of glucose metabolism, playing a hypoglycaemic effect. Insulin-glucagon coordination is essential for glucose homeostasis. Insulin resistance and loss of beta-cell function are involved in the development of type 2 diabetes mellitus (T2DM).