Recombinant Xenopus laevis Brachyury protein (t)

Code CSB-YP023052XBE
MSDS
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Source Yeast
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Code CSB-EP023052XBE
MSDS
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Source E.coli
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Code CSB-EP023052XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP023052XBE
MSDS
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Source Baculovirus
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Code CSB-MP023052XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
t
Uniprot No.
Alternative Names
tbxt; bra; t; T-box transcription factor T; Brachyury protein; xBRA; Protein T
Species
Xenopus laevis (African clawed frog)
Expression Region
1-432
Target Protein Sequence
MSATESCAKN VQYRVDHLLS AVENELQAGS EKGDPTEKEL KVSLEERDLW TRFKELTNEM IVTKNGRRMF PVLKVSMSGL DPNAMYTVLL DFVAADNHRW KYVNGEWVPG GKPEPQAPSC VYIHPDSPNF GAHWMKDPVS FSKVKLTNKM NGGGQIMLNS LHKYEPRIHI VRVGGTQRMI TSHSFPETQF IAVTAYQNEE ITALKIKHNP FAKAFLDAKE RNDYKDILDE GIDSQHSNFS QLGTWLIPNG GSLCSPNPHT QFGAPLSLSS PHGCERYSSL RNHRSAPYPS PYTHRNNSPN NLADNSSACL SMLQSHDNWS TLQMPAHTGM LPMSHSTGTP PPSSQYPSLW SVSNSAITPV SQSGGITNGI SSQYLLGSTP HYSSLSHAVP SPSTGSPLYE HGAQTEIAEN QYDVTAHSRL SSTWTPVAPP SV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence. Causes dorsal mesodermal differentiation of animal cap ectoderm when co-expressed with wnt8 and noggin. None of these molecules causes dorsal mesoderm formation when expressed alone. Establishes the left/right axis at early gastrula stage by directly up-regulating mesodermal expression of zic3.
Gene References into Functions
  1. Disrupting the pattern of division by placing embryos in a temperature gradient resulted in highly asynchronous entry to the midblastula transition and misexpression of the mesodermal protein Xbra. PMID: 28978482
  2. DN-Xbra inhibits PV.1 expression and that the suppression of PV.1 reduces overall BMP signaling by inducing chordin and goosecoid expression. PMID: 24499677
  3. controls the early homeobox gene expression domain in the animal-vegetal direction PMID: 14729483
  4. findings reveal that the N-terminal domain that restricts the inducing activity of Xbra interacts with Smad1 PMID: 15809041
  5. Embryo elongation can occur through the synergistic effect(s) of the organizer molecule chordin, and the 'verall posteriorizing molecule Xbra. PMID: 18318733

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in presumptive mesodermal cells around the blastopore, and then in the notochord.
Database Links

KEGG: xla:399275

UniGene: Xl.514

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