Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

TRAC

Uniprot No.

P01848

Research Area

Immunology

Alternative Names

TRAC; TCRA; T cell receptor alpha constant

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-142aa

Target Protein Sequence

PNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESSCDVKLVEKSFETDTNLNFQNLSVIGFRILLLKVAGFNLLMTLRLWSS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

19.9kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The Recombinant Human TRAC protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of TRAC gene. This recombinant TRAC protein was expressed in the host. The expression region is 1-142aa of the Human TRAC. In the production, the expression vector contains N-terminal 6xHis tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.

The TRAC gene encodes for the constant region of the TCR subunit and is essential for membrane expression of the TCRαβ heterodimer. In recent years, researcher revealed the important roles of TRAC in different diseases. For example, it has been found that mutation in the TCRα subunit constant gene leads to a human immunodeficiency disorder characterized by a lack of TCRαβ+ T cells. Besides, narcolepsy is strongly associated with the T-cell receptor alpha locus. Furthermore, gene rearrangements and lymphoma are partly responsible for a pathogenic TRAC Variant. Previous studies have shown that mutation in TCRα subunit constant (TRAC) leads to loss of αβ TCR on T-cell surface. Beyond this, some findings indicate that CRISPR-Cas9-mediated multiplex gene editing of TRAC, B2M, and PD-1 in CAR-T cells. The reports further adds that although these double (TRAC/B2M)- and triple (TRAC/B2M/PD-1)-negative CAR-T cells need to be further tested for their safety and efficacy in clinical studies, our results suggest that they are promising reagents for cancer therapy.

Target Background

Function

Constant region of T cell receptor (TR) alpha chain. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity.

Involvement in disease

Immunodeficiency 7 (IMD7)

Subcellular Location

Cell membrane.

Database Links

HGNC: 12029

OMIM: 186880

UniGene: Hs.74647

Code	CSB-EP024144HU
Abbreviation	Recombinant Human TRAC protein
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human T cell receptor alpha chain constant (TRAC)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage