Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
TRAC; TCRA; T cell receptor alpha constant
Species
Homo sapiens (Human)
Expression Region
1-142aa
Target Protein Sequence
PNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESSCDVKLVEKSFETDTNLNFQNLSVIGFRILLLKVAGFNLLMTLRLWSS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The Recombinant Human TRAC protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of TRAC gene. This recombinant TRAC protein was expressed in the host. The expression region is 1-142aa of the Human TRAC. In the production, the expression vector contains N-terminal 6xHis tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.
The TRAC gene encodes for the constant region of the TCR subunit and is essential for membrane expression of the TCRαβ heterodimer. In recent years, researcher revealed the important roles of TRAC in different diseases. For example, it has been found that mutation in the TCRα subunit constant gene leads to a human immunodeficiency disorder characterized by a lack of TCRαβ+ T cells. Besides, narcolepsy is strongly associated with the T-cell receptor alpha locus. Furthermore, gene rearrangements and lymphoma are partly responsible for a pathogenic TRAC Variant. Previous studies have shown that mutation in TCRα subunit constant (TRAC) leads to loss of αβ TCR on T-cell surface. Beyond this, some findings indicate that CRISPR-Cas9-mediated multiplex gene editing of TRAC, B2M, and PD-1 in CAR-T cells. The reports further adds that although these double (TRAC/B2M)- and triple (TRAC/B2M/PD-1)-negative CAR-T cells need to be further tested for their safety and efficacy in clinical studies, our results suggest that they are promising reagents for cancer therapy.