Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
TRAC; TCRA; T cell receptor alpha constant
Species
Homo sapiens (Human)
Expression Region
1-142aa
Target Protein Sequence
PNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESSCDVKLVEKSFETDTNLNFQNLSVIGFRILLLKVAGFNLLMTLRLWSS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Intact human T cell receptor alpha constant (TRAC) cDNA (1-142aa) with an N-terminal 6xHis-tag was expressed in the yeast. The forming protein is the recombinant full-length human TRAC protein. The purity of this protein is greater than 90% determined by SDS-PAGE. Under reducing conditions, the gel showed a molecular weight band of about 16 kDa. This recombinant TRAC protein can be used as the immunogen for antibody synthesis. Besides, it also may be applied in the studies of TRAC-related immunology.
The extracellular region of αβ T cell receptors (TCRs) is made up of an α chain and a β chain, each containing Ig-like variable and constant domains. TRAC, the TCR Cα domain, is significant in differing from the classical IgC1 domain. Substantial evidence indicated that TRAC interfaces with CD3. Mutagenesis has demonstrated that the interactions of CD3δɛ and CD3γɛ subunits with the TcR Cα and Cβ domains, respectively, contribute to the stability and function of the TCR–CD3 signaling complex. Gijs I. van Boxel etc. concluded that the TCR Cα domain can also adopt two very different stable conformations, a fundamental property that is potentially central to TCR function and that the TCR Cα domain may facilitate the "β-strand slippage."
