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Fish Cortisol ELISA Kit

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Citations (75) Protocol
Code CSB-E08487f
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Abbreviation
COR
Species
Fish
Sample Types
serum, plasma, tissue homogenates.
Detection Range
3.12 ng/ml-200 ng/ml.
Sensitivity
1.56 ng/ml.
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
 
Linearity
 
Recovery
 
Typical Data
 
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Citations

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ELISA kits

Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Fish

Sample dilution: No dilution

Review: I used the kit CSB-E08487f to test tilapia serum under temperature stress. The serum was not diluted. The test results showed that the standard curve was normal, and the cortisol content in the sample was in the range of 50-200ng/mL. The experimental methods and steps of the product are detailed, and also provide solutions to possible problems and warm tips, which are very humane and scientific. However, most of the solution in the kit has a large amount of remaining after the use of the 96-well plate, and the number of well plates coated with antibodies is small. It is suggested that the number of well plates can be increased and the amount of some reagents can be reduced.

By Anonymous

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■ Q&A
Q:

1) Does the kit come with PBS -- is that what the included was solution is? Would Tris-HCL work as a wash solution? It is what my lab wrote they used in the pilot study but I do not have any context for why they chose it over PBS.
2) For getting tissue homogenates, do you happen to have any recommendations for grinding the tissue?

A:

This kit (CSB-E08487f) is ready to use and provides all required reagents. No extra solution is required. Tris-HCl you mentioned may be not suitable for this kit. Please use the matched reagents of this kit， including the wash buffer.
For the processing of tissue samples, you need to prepare 1×PBS for homogenization treatment, use a conventional tissue grinding method, and then perform a gradient pre-experiment. For details, please refer to the sample processing section of the manual.

Q:

Does this kit measure total cortisol levels, or only free/unbound cortisol? I will be using zebrafish tissue homogenates but I am not seeing any information in the protocol about whether it is total or only free cortisol.

A:

This is a competitive kit, and it detects endogenous total cortisol. For zebrafish tissue samples, it is recommended to arrange a gradient pre-experiment.

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