Recombinant Rat Amyloid beta A4 protein(App),partial

Code CSB-YP001950RA1
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Source Yeast
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Code CSB-EP001950RA1
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Source E.coli
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Code CSB-EP001950RA1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001950RA1
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Source Baculovirus
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Code CSB-MP001950RA1
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names App
Uniprot No. P08592
Alternative Names AppAmyloid-beta A4 protein; ABPP; APP; Alzheimer disease amyloid A4 protein homolog; Amyloid precursor protein; Amyloid-beta precursor protein; Amyloidogenic glycoprotein; AG) [Cleaved into: N-APP; Soluble APP-alpha; S-APP-alpha); Soluble APP-beta; S-APP-beta); C99; Beta-secretase C-terminal fragment; Beta-CTF); Amyloid-beta protein 42; Abeta42; Beta-APP42); Amyloid-beta protein 40; Abeta40; Beta-APP40); C83; Alpha-secretase C-terminal fragment; Alpha-CTF); P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59; Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57; Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50; Gamma-CTF(50)); C31]
Species Rattus norvegicus (Rat)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions (By similarity). Can promote transcription activation through binding to APBB1-KAT5 and inhibit Notch signaling through interaction with Numb (By similarity). Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV (By similarity). The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1 (By similarity).
Gene References into Functions
  1. These results, in Wistar rats, provide experimental support for the hypothesis that certain risk factors, such as energy metabolism dysfunction or the aging process itself, may increase vulnerability to beta-amyloid protein (Abeta). Hippocampal region is more susceptible to Abeta and its effect increases with age in relation to the neocortex. PMID: 29624358
  2. Abeta 25-35 was found to induce PC12 cell apoptosis in a dose-dependent manner (P<0.05). Moreover, Abeta 25-35 also caused dose-dependent disintegration of the cytoskeleton (P<0.05). PMID: 29436599
  3. Andrographolide (ANDRO) reduced inflammation-mediated neuronal damage by blocking inflammatory responses of microglial cells to Abeta(1-42), suggesting ANDRO may be an effective agent in modulating neuroinflammatory process in Alzheimer's disease. PMID: 28669260
  4. alanine in APP- intracellular domain(ICD) and the proline in APLP2-ICD lie directly behind a conserved caspase cleavage site PMID: 26921470
  5. These data suggest that methylation change in BDNF exon is involved in the regulation of BDNF expression by Abeta or SAM, and further support the view of specific epigenetic modifications of a certain BDNF gene transcript. PMID: 29305263
  6. Results from the present study indicated that pioglitazone may improve insulin sensitivity and ameliorate Abeta42 accumulation in rats with dietinduced IR by regulating AKT/GSK3beta activation, suggesting that pioglitazone may be a promising drug for AD treatment. PMID: 28447730
  7. Dynamic transport of Abeta aggregates is only observed between axon terminal and cell body. In addition to differential cellular uptake, more Abeta-peptide secretion is detected significantly from axons than from dendritic side. PMID: 28121396
  8. Results show that concerted signaling by both APP and Abeta is necessary for aberrant neuronal cell cycle entry. Thus, APP plays a central role in promotion of neurodegeneration, through activation of Ras-ERK signaling axis as well as GSK-3, and interfering with these signaling events would impede cell cycle reentry and neurodegeneration observed in Alzheimer's disease. PMID: 28374012
  9. Lynx1 and Abeta1-42 bind competitively to multiple nicotinic acetylcholine receptor subtypes. PMID: 27460145
  10. increased APP and/or beta-CTF impact the endocytic pathway to disrupt NGF trafficking and signaling, resulting in trophic deficits in basal forebrain cholinergic neurons. PMID: 27064279
  11. results establish a novel toxic impact ofAbeta oligomers on neuronal metabolism and suggest that Abeta oligomers-induced, NMDA receptor-mediated AMPK inhibition may play a key role in early brain metabolic defects in AD. PMID: 28302722
  12. The protein level of Abeta in serum and cerebrospinal fluid (CSF) was severity and timedependent during the acute phase in rats with traumatic spinal cord injury (SCI). Monitoring the level of Abeta protein in serum may improve the evaluation of SCI severity and the neuron functional status following SCI. PMID: 28259979
  13. O3 exposure (0.25 ppm) induced a significant increase of Syntaxin 5 and accumulation of betaA42 peptide in the reticulum in cells of the dentate gyrus PMID: 27366738
  14. results indicate that specifically "trapping" low-n oligomers provides a novel strategy for toxic Abeta42-oligomer recognition and removal. PMID: 26510576
  15. Results indicate that improving synaptic soluble AbetaPPalpha availability at synapses helps in reducing the functional NMDAR-related deregulation of hippocampal networks linked to aging PMID: 26402095
  16. High glucose leads to the increased expression of genes related to Abeta production, resulting in the accumulation of Abeta in the lens. PMID: 27028062
  17. Autoimmune Optic Neuritis Is Associated with Altered APP Cleavage in Neurons and Up-Regulation of p53 PMID: 26426258
  18. Redox-mediated posttranslational modification of brain proteins link Abeta and hyperglycaemia to cognitive dysfunction in metabolic syndrome/type 2 diabetes and Alzheimer disease. PMID: 26743041
  19. This study indicates that interfering intracellular Abeta especially mitochondrial Abeta accumulation, together with ameliorating Abeta-associated mitochondrial dysfunction, may contribute to the protective effects of huperzine A against Abeta neurotoxicity. PMID: 26024517
  20. Syt-1 and Syt-9 regulate endogenous APP-CTF and Abeta levels in PC12 cells. Secreted sAPPbeta levels were significantly reduced in PC12 cells lacking Syt-1 expression. PMID: 26202512
  21. Results show that downregulation of let-7d might contribute to isoflurane-induced learning and memory impairment through upregulating its target APP, and increasing the production of Abeta subsequently. PMID: 25799420
  22. Exposure to As-, Cd-, and Pb-mixture induces Abeta, amyloidogenic APP processing and cognitive impairments via oxidative stress-dependent neuroinflammation in young rats. PMID: 25288670
  23. Data suggests that APP is important in regulating intestinal cholesterol uptake in a fashion dependent upon specific proteolytic pathways. PMID: 25742317
  24. The soluble extracellular fragment of neuroligin-1 protects synapses in neurons damaged by ABETA oligomers. PMID: 26325471
  25. The present study indicates that Abeta-mediated Zn2+ influx into dentate granule cells, which may occur without AMPA receptor activation, transiently induces a short-term cognitive deficit. PMID: 25536033
  26. Incubation of brain homogenates with 70 microM hydrogen peroxide significantly influenced the profile of Abeta binding proteins and 0.1 mM isatin decreased the number of identified Abeta binding proteins. PMID: 25551598
  27. Abeta-40 Y10F exhibited remarkably decreased neurotoxicity compared to Abeta-40 which could be partly due to the reduced generation of hydrogen peroxide. PMID: 22754299
  28. The results of this study provide the first direct demonstration that AbetaPP is mostly distributed among glutamatergic rather than GABAergic or cholinergic terminals of the adult rat hippocampus. PMID: 24531160
  29. report we describe a novel neuroprotective function of mGlu3 receptors related to their ability to promote the non-amyloidogenic pathway of APP cleavage in astrocytes, thus enhancing sAPPalpha production PMID: 24291464
  30. Rodent-Abeta staining is present and increased after juvenile traumatic brain injury around cerebral blood microvessels, and the diameter of those is decreased by 25% and 34% at 2 and 6 months, respectively, without significant angiogenesis. PMID: 25052558
  31. Oxidative stress served as the key trigger to down-regulate miR-107 by cell- derived soluble Abeta. PMID: 24595404
  32. present results suggest that the amyloid peptide exhibits a protective antioxidant role in biological systems, but also has toxic actions independent of oxidative stress PMID: 23041348
  33. Both voltage-activated Ca2+ channel and NMDAR-dependent long-term potentiation in vivo are strongly inhibited by Abeta. PMID: 24298149
  34. the existence of a structurally related family of quasi-fibrillar conformers of Abeta42, which is stabilized both by curcumin and by Zn(2+.) PMID: 24599958
  35. analysis of the role of JIP1 in APP transport; knockdown of JIP1 did not affect either amyloid precursor protein transport or amyloid-beta peptide production PMID: 23825109
  36. Data suggest that the brain expression of neprilysin may promote amyloid beta-protein (Abeta)deposition in patients with Alzheimer's disease (AD). PMID: 24508800
  37. Neurodegeneration may occur in a retrograde fashion from axon terminals near beta-amyloid plaques which may spread through brain regions in cortical neuron cultures. PMID: 24114864
  38. APP is an independently operating cell adhesion molecule PMID: 23691241
  39. These data present novel insights into the subsynaptic localization of APPs PMID: 23815291
  40. Abeta42 globulomers have an antiparallel beta-sheet arrangement PMID: 23687299
  41. processing of amyloid precursor protein in cells undergoing apoptosis PMID: 23469123
  42. Identification of the minimal zinc-binding center in natural isoforms of amyloid-beta domain 1-16 using ESI-MS PMID: 23888782
  43. PuF may regulate the APP gene promoter PMID: 23368879
  44. results showed that EMP exposure can cause long-term impairment in impaired cognition and memory of rats, resulting in AD-like symptoms; this may be induced by enhancing oxidative stress and is related to autophagy dysfunction PMID: 23523687
  45. The CentA1-Ras-Elk-1 signaling pathway acts on mitochondria to regulate dendritic spine density and synaptic plasticity in response to Abeta in hippocampal neurons. PMID: 23516302
  46. Prion protein-mediated toxicity of amyloid-beta oligomers requires lipid rafts and the transmembrane LRP1 PMID: 23386614
  47. Neuronal binding and hippocampal neurotransmission were found to be suitable to account for the synaptotoxicity to different App assemblies, based on the stability of the applied App aggregates in these settings. PMID: 23374097
  48. Early brain injury alters the blood-brain barrier phenotype in parallel with beta-amyloid and cognitive changes in adulthood. PMID: 23149553
  49. S-nitrosylation pathway wherein Cdk5/NOS1 interaction enhances S-nitrosylation of Cdk5, mediates mitochondrial dysfunction and synaptic loss during the App etiology of Alzheimer's disease. PMID: 22874667
  50. C1q-induced LRP1B and GPR6 proteins expressed early in Alzheimer disease mouse models, are essential for the C1q-mediated protection against amyloid-beta neurotoxicity PMID: 23150673

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Subcellular Location Membrane, Single-pass type I membrane protein, Membrane, clathrin-coated pit
Protein Families APP family
Tissue Specificity In the brain, non-L-APP isoforms are expressed in neurons, isoform APP695 being the predominant form. In astrocytes and microglial cells, almost 50% is L-isoform (appican).
Database Links

KEGG: rno:54226

STRING: 10116.ENSRNOP00000041613

UniGene: Rn.2104

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