kn Antibody

Code CSB-PA348728XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) kn Polyclonal antibody
Uniprot No.
Target Names
kn
Alternative Names
kn antibody; col antibody; CG10197Transcription factor collier antibody; Transcription factor knot antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) kn protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
May act as a 'second-level regulator' of head patterning. Required for establishment of the PS(-1)/PS0 parasegmental border and formation of the intercalary segment. Required for expression of the segment polarity genes hedgehog, engrailed and wingless, and the segment-identity genes CAP and collar in the intercalary segment. Required at the onset of the gastrulation for the correct formation of the mandibular segment.
Gene References into Functions
  1. two independent sites of Collier/Early B Cell Factor expression, hematopoietic progenitors and the PSC, achieve control of hematopoiesis PMID: 26866694
  2. Col direct control of expression of the transcription regulators apterous and eyes-absent is critical to specifying neuronal identities. cross-regulation between col and eya in muscle progenitor cells is required for specification of muscle identity PMID: 26204530
  3. Col expression is required in the prohemocytes to prevent their differentiation. PMID: 26150488
  4. Collier controls hemocyte homeostasis via coordinate regulation of PSC cell number and PSC signaling to prohemocytes. PMID: 22331866
  5. Second order regulator Collier directly controls intercalary-specific segment polarity gene expression. PMID: 22005665
  6. Connecting Hh, Dpp and EGF signalling in patterning of the Drosophila wing; the pivotal role of this protein in the AP organizer PMID: 12183378
  7. collier has a role in the cellular immune response to parasitization in Drosophila PMID: 15314643
  8. two apparently functionally redundant blocks of sequences for repression by UBX PMID: 15753212
  9. Kn exerts its cell-autonomous function to control the formation, and possibly the function, of class IV-like elaborated dendritic arbors. PMID: 17825045
  10. Characterization of the col upstream region driving DA3 muscle specific expression revealed the existence of three separate phases of cis-regulation, correlating with conserved binding sites for different mesodermal transcription factors. PMID: 18003742
  11. Knot increases dendritic arbor outgrowth through promoting the expression of Spastin, a microtubule-severing protein in Drosophila. PMID: 18093520
  12. Control of multidendritic neuron differentiation in Drosophila: the role of kn. PMID: 18234173

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Subcellular Location
Nucleus.
Protein Families
COE family
Tissue Specificity
Its expression at the blastoderm stage is restricted to a single stripe of cells corresponding to part of the intercalary and mandibular segment primordia, possibly parasegment O.
Database Links

KEGG: dme:Dmel_CG10197

STRING: 7227.FBpp0111722

UniGene: Dm.1803

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