CS Antibody

Code CSB-PA006031LA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA006031LA01HU at dilution of 1:100

  • Western blot
    All lanes: CS antibody at 3µg/ml + HepG2 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa

  • Western Blot
    Positive WB detected in: Zebrafish tissue
    All lanes: CS antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 52 kDa
    Observed band size: 52 kDa

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CS Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
CISY_HUMAN antibody; Citrate synthase antibody; Citrate synthase; mitochondrial antibody; citrate synthetase antibody; Cs antibody; EC 2.3.3 antibody; EC 2.3.3.1 antibody
Raised in
Rabbit
Species Reactivity
Human, Zebrafish
Immunogen
Recombinant Human Citrate synthase, mitochondrial protein (28-464AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CS Antibody (Product code: CSB-PA006031LA01HU) is Non-conjugated. For CS Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA006031LB01HU CS Antibody, HRP conjugated ELISA
FITC CSB-PA006031LC01HU CS Antibody, FITC conjugated
Biotin CSB-PA006031LD01HU CS Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Gene References into Functions
  1. citrate synthase and ACSS1 have tumorigenic functions in hepatocellular carcinoma PMID: 27363021
  2. Citrate synthase activity was lower in patients with heart failure with preserved ejection fraction compared to controls. PMID: 27179829
  3. mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers. PMID: 27793029
  4. METTL12 methylates CS on Lys-395, which is localized in the CS active site. Interestingly, the METTL12-mediated methylation inhibited CS activity and was blocked by the CS substrate oxaloacetate. PMID: 28887308
  5. Data suggest that METTL12, localized in mitochondrial matrix, modifies/methylates Lys368 of citrate synthase in external surface region close to its catalytic site; addition/removal of methylation has no effect on citrate synthase activity; Lys368 occurs in highly conserved sequence of amino acid residues of citrate synthase. (METTL12 = methyltransferase-like protein 12) PMID: 28391595
  6. Data suggest that downregulation of citrate synthase (CS) expression in 293T cells leads to low level of ATP production, excessive superoxide formation and cell apoptosis, which implies a possible mechanism for hearing loss in A/J mice. PMID: 28216161
  7. Hence, we conclude that SIRT3 exhibits neuroprotection via deacetylating and increasing mitochondrial enzyme activities. PMID: 28161643
  8. Paretic muscle in hemiparetic stroke survivors had lower CS concentration. PMID: 26361074
  9. The citrate synthase knockdown cells exhibited severe defects in respiratory activity and marked decreases in ATP production, but great increases in glycolytic metabolism. PMID: 23139858
  10. Citrate synthase (CS) is a direct RORalpha target gene and one mechanism by which RORalpha regulates lipid metabolism is via regulation of CS expression. PMID: 22485150
  11. Responses of skeletal and cardiac muscles in CS activity and gene expression at 1 and 48 h after endurance training. Acute effect of exercise on training-induced elevation in CS activity in rat soleus but not ventricle muscles. PMID: 12531911
  12. possible role of CS-specific autoantibodies in the pathomechanism of allograft vasculopathy PMID: 15711981
  13. It is likely that enhanced citrate synthase activity contributes to the conversion of glucose to lipids in pancreatic cancer providing substrate for membrane lipids synthesis. PMID: 15714131
  14. These results provide an important basis for the study of mitochondrial dysfunction due to aberrant CSa trafficking. PMID: 19479947

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Subcellular Location
Mitochondrion matrix.
Protein Families
Citrate synthase family
Database Links

HGNC: 2422

OMIM: 118950

KEGG: hsa:1431

STRING: 9606.ENSP00000342056

UniGene: Hs.743252

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