Cortisol is a steroid hormone produced by the adrenal cortex and serves as a primary glucocorticoid in mammals. It plays essential roles in glucose metabolism, immune response regulation, and stress response pathways. Cortisol levels fluctuate in response to circadian rhythms and various physiological stressors, making it a valuable biomarker for studying metabolic disorders, stress-related conditions, and endocrine function. Research applications include studies of hypothalamic-pituitary-adrenal axis activity, behavioral studies, and disease models where cortisol dysregulation is implicated.
The Mouse Cortisol ELISA Kit (CSB-E05113m) provides quantitative measurement of cortisol in Mus musculus samples using a competitive assay principle. The kit requires 50-100 μl sample volume and delivers results within 1-5 hours. Detection is performed at 450 nm wavelength, allowing researchers to measure cortisol levels in mouse models for endocrine and stress-related studies.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This mouse cortisol ELISA kit has been used in research studying stress response mechanisms and metabolic regulation. Studies have applied the kit to measure cortisol levels in serum and plasma samples from mouse models, particularly in contexts examining the interplay between stress hormones and physiological processes.
• Metabolic research: Measurement of cortisol levels alongside glucose tolerance testing, insulin resistance measurements, and other metabolic parameters to understand stress-metabolism interactions
• Sleep and circadian studies: Measurement of plasma cortisol concentrations in sleep deprivation experiments, with careful consideration of circadian rhythm effects on hormone levels
• Stress hormone profiling: Measurement of cortisol as part of comprehensive stress hormone panels that include other neuroendocrine markers
• Endocrine research: Study of hormonal responses in experimental models, including studies that account for reproductive cycle influences on hormone concentrations
