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Mouse Hepatocyte Growth Factor Receptor(C-MET/HGFR)ELISA Kit

Mouse Hepatocyte Growth Factor Receptor(C-MET/HGFR)ELISA Kit

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Citations (5) Protocol MSDS

Code	CSB-E13492m
Size	96T,5×96T,10×96T
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Product Details
Citations (5)
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Target Background

Product Details

Target Name

met proto-oncogene (hepatocyte growth factor receptor)

Alternative Names

Met ELISA Kit; Hepatocyte growth factor receptor ELISA Kit; HGF receptor ELISA Kit; EC 2.7.10.1 ELISA Kit; HGF/SF receptor ELISA Kit; Proto-oncogene c-Met ELISA Kit; Scatter factor receptor ELISA Kit; SF receptor ELISA Kit; Tyrosine-protein kinase Met ELISA Kit

Abbreviation

MET

Uniprot No.

P16056

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.078 ng/mL-5 ng/mL

Sensitivity

0.02 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	639.988	613.434	633.318	588.302	625.563	621.144
SD	0.035	0.040	0.036	0.045	0.048	0.052
CV(%)	4.698	5.548	4.871	6.447	6.557	7.143

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-MET/HGFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-96
1:2	Average %	102
	Range %	96-107
1:4	Average %	91
	Range %	87-96
1:8	Average %	95
	Range %	89-99

Recovery

The recovery of mouse C-MET/HGFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-101
EDTA plasma (n=4)	104	92-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
5	2.723	2.854	2.789	2.701
2.5	1.856	1.987	1.922	1.834
1.25	1.219	1.264	1.242	1.154
0.625	0.715	0.732	0.724	0.636
0.312	0.399	0.386	0.393	0.305
0.156	0.248	0.255	0.252	0.164
0.078	0.158	0.162	0.160	0.072
0	0.087	0.088	0.088

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The met proto-oncogene encodes the hepatocyte growth factor receptor (HGFR), also known as C-MET, a receptor tyrosine kinase that regulates cellular proliferation, survival, motility, and morphogenesis. When hepatocyte growth factor binds to MET, the receptor dimerizes and undergoes autophosphorylation. This activates downstream signaling cascades including PI3K/AKT and MAPK pathways. The receptor is essential for embryonic development, tissue regeneration, and wound healing. Its dysregulation contributes to various pathological conditions including cancer metastasis and tumor progression.

The Mouse Hepatocyte Growth Factor Receptor ELISA Kit (CSB-E13492m) provides quantitative measurement of MET in Mus musculus samples using a sandwich assay principle. This kit works with serum, plasma, and tissue homogenates. The detection range spans 0.078 ng/mL to 5 ng/mL with sensitivity of 0.02 ng/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining hepatocyte growth factor receptor expression in various biological contexts. Studies have applied the kit to quantify receptor levels in tissue homogenates and serum samples, supporting research into mesenchymal-epithelial transition processes and related cellular signaling pathways.

• Tumor biology research: Quantification of hepatocyte growth factor receptor in primary tumor tissue homogenates to measure mesenchymal-epithelial transition factor expression
• Serum biomarker analysis: Measurement of circulating hepatocyte growth factor receptor levels in serum samples using sandwich immunoassay methodology
• Multi-target profiling: Integration with other biomarker measurements to provide comprehensive molecular characterization in research studies

Citations

PDCD5 regulates cell proliferation, cell cycle progression and apoptosis Li P,Oncology letters,2023
Effects of Wnt‑1 blockade in DEN‑induced hepatocellular adenomas of mice Sklavos A,Oncology letters,2023
HDAC1/2 Inhibitor Romidepsin Suppresses DEN-Induced Hepatocellular Carcinogenesis in Mice H Afaloniati,OncoTargets and Therapy,2020
Novel aminochromone derivative inhibits tumor growth on xenograft model of lung cancer in mice Ekaterina V Blinova, et al,Journal of Advanced pharmaceutical technology research,2018
Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation Wang X.et al,Cytokine,2017

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Target Background

Function
(From Uniprot)

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis.; (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.

Involvement in disease

Activation of Met after rearrangement with the TPR (translocated promoter) locus of chromosome 1 produces an oncogenic protein.

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Tyr protein kinase family

Database Links

STRING: 10090.ENSMUSP00000079324

UniGene: Mm.86844

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