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Mouse Hepatocyte Growth Factor Receptor(C-MET/HGFR)ELISA Kit

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Citations (5) Protocol MSDS
Code CSB-E13492m
Size 96T,5×96T,10×96T
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Product Details

Target Name
met proto-oncogene (hepatocyte growth factor receptor)
Alternative Names
Met ELISA Kit; Hepatocyte growth factor receptor ELISA Kit; HGF receptor ELISA Kit; EC 2.7.10.1 ELISA Kit; HGF/SF receptor ELISA Kit; Proto-oncogene c-Met ELISA Kit; Scatter factor receptor ELISA Kit; SF receptor ELISA Kit; Tyrosine-protein kinase Met ELISA Kit
Abbreviation
MET
Uniprot No.
P16056
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.078 ng/mL-5 ng/mL
Sensitivity
0.02 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

20

20

20

20

20

20

Mean(pg/ml)

639.988

613.434

633.318

588.302

625.563

621.144

SD

0.035

0.040

0.036

0.045

0.048

0.052

CV(%)

4.698

5.548

4.871

6.447

6.557

7.143

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-MET/HGFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

　

Sample

Serum(n=4)

1:1

Average %

91

Range %

87-96

1:2

Average %

102

Range %

96-107

1:4

Average %

91

Range %

87-96

1:8

Average %

95

Range %

89-99

Recovery

The recovery of mouse C-MET/HGFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

97

92-101

EDTA plasma (n=4)

104

92-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

5

2.723

2.854

2.789

2.701

2.5

1.856

1.987

1.922

1.834

1.25

1.219

1.264

1.242

1.154

0.625

0.715

0.732

0.724

0.636

0.312

0.399

0.386

0.393

0.305

0.156

0.248

0.255

0.252

0.164

0.078

0.158

0.162

0.160

0.072

0

0.087

0.088

0.088

　

ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The mouse hepatocyte growth factor receptor (HGFR) ELISA kit is suitable for quantitatively determining mouse HGFR in serum, plasma, or tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify mouse HGFR levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

HGFR, also called MET/c-MET, is stimulated upon HGF binding and further activates downstream effector molecules, thus eliciting Grb2-Ras-Raf-Erk/MAPK, Gab1-Shp2-Erk/MAPK, PI3K-AKT, and STAT3 signaling pathway. HGF/c-MET signaling is involved in different processes including normal development, organ regeneration, cell cycle progression, cell proliferation, survival, migration, invasion, and tumorigenesis. Dysregulation of HGF/cMET signaling plays a crucial role in several human cancers, especially in invasion and metastasis.

Citations

Related Products

Met Proteins

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis.; (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.
Involvement in disease
Activation of Met after rearrangement with the TPR (translocated promoter) locus of chromosome 1 produces an oncogenic protein.
Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Tyr protein kinase family
Database Links
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