Product Details

Purity

>85% (SDS-PAGE)

Target Names

tim

Uniprot No.

P49021

Alternative Names

tim; CG3234; Protein timeless

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Required for the production of circadian rhythms. The biological cycle depends on the rhythmic formation and nuclear localization of the TIM-PER complex. Light induces the degradation of TIM, which promotes elimination of PER. Nuclear activity of the heterodimer coordinatively regulates PER and TIM transcription through a negative feedback loop. Behaves as a negative element in circadian transcriptional loop. Does not appear to bind DNA, suggesting indirect transcriptional inhibition.

Gene References into Functions

data suggest that CRTC is a novel co-activator for the CLK/CYC-activated tim transcription to coordinate molecular rhythms with circadian behaviors over a 24-hour time-scale. PMID: 27577611
A phosphorylation program, native to the master pacemaker neurons, regulates the timing of nuclear accumulation of the Period/Timeless repressor complex. PMID: 27346344
mir-276a inhibits tim expression: Deleting the mir-276a-binding site in the tim 3' UTR causes elevated levels of TIM and approximately 50% arrhythmicity. PMID: 27162360
possible roles it plays in the physiological preservation of health, as well as in the pathogenic mechanisms of related diseases [review] PMID: 26691298
CULLIN-3 is required for the circadian control of PERIOD and TIMELESS oscillations. PMID: 22879814
Nuclear TIM expression is associated with day-night cycles that are independent of the central nervous system. PMID: 22713751
Timeless regulates phagocytosis of bacteria in Drosophila. PMID: 22253593
This study demonstrated that timelss mutant affect TIMELESS and PERIOD post-trnaslational regulation and metabolism in cell nuclear. PMID: 21734289
structural domains PMID: 12372263
Tim1 has a role in sister chromatid cohesion. PMID: 12852847
Regulation of the timeless protein involves nuclear transport mechanisms. PMID: 12944510
A novel tim mutation affects behavioral rhythms but not periodic eclosion. PMID: 15520259
results suggest that JET is essential for resetting the circadian clock by transmitting light signals from cryptochrome to TIMELESS PMID: 16794082
Results suggest that photic stimulation of timeless expression at low temperatures is part of a seasonal adaptive response that helps advance the clock phase on cold days, enabling flies to exhibit daytime activity despite the earlier onset of dusk. PMID: 16864646
Data show that ectopic CRYPTOCHROME renders TIMELESS light-sensitive in the Drosophila ovary. PMID: 16864647
show a strong interaction between Veela and cryptochrome genetic variants, demonstrating that the Jetlag, Timeless, and cryptochrome proteins function in the same pathway PMID: 17068124
study reports that a mutation in the timeless gene has arisen and spread by natural selection relatively recently in Europe; natural and artificial alleles of the timeless gene affect the incidence of diapause in response to changes in light & temperature PMID: 17600215
findings show that the mutant allele attenuates the photosensitivity of the circadian clock and causes decreased dimerization of the mutant TIMELESS protein isoform to CRYPTOCHROME; this interaction results in a more stable TIMELESS product PMID: 17600216
these data indicate that TIM is an important mediator of CK2 effects on circadian rhythms. PMID: 18815259

Hide All

Subcellular Location

Nucleus. Cytoplasm, perinuclear region. Note=Nuclear at specific periods of the day. First accumulates in the perinuclear region about one hour before translocation into the nucleus. Interaction with per is required for nuclear localization.

Protein Families

Timeless family

Tissue Specificity

Expressed in head, photoreceptors, lateral neurons and glial cells in the lamina and medulla of the optic lobes. Expression follows a light-dark cycle, levels show a significant decrease at the end of the night and then remain low throughout the light per

Database Links

KEGG: dme:Dmel_CG3234

STRING: 7227.FBpp0305454

UniGene: Dm.2663

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
If CAPTCHA verification fails, click to refresh the CAPTCHA and try again.

Code	CSB-YP342197DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP342197DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP342197DLU-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP342197DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP342197DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Drosophila melanogaster Protein timeless (tim), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage