Rat cartilage oligomeric matrix protein,COMP ELISA Kit

Code CSB-E13833r
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The product CSB-E13833r is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of rat cartilage oligomeric matrix protein (COMP) in biological samples, including serum, plasma, or tissue homogenates. This kit has undergone rigorous quality tests in multiple parameters, including specificity, sensitivity, precision, and lot-to-lot consistency.

The detection mechanism of this kit is based on the Sandwich-ELISA technique. COMP in the samples or standards are sandwiched between pre-coated COMP antibody and Biotin-conjugated COMP antibody. The COMP/pre-coated COMP antibody/Biotin-conjugated COMP antibody complex is labeled by HRP and then occurs a chromogenic reaction after the addition of the TMB solution. The color intensity is positively related to the COMP in the samples.

COMP, also known as thrombospondin-5 (TSP-5), is a matricellular protein essential for maintaining vascular homeostasis. It also plays a crucial role in cartilage and bone metabolism and is involved in arthritis. COMP preserves the contractile phenotype of vascular smooth muscle cells and prevents the formation of vascular neointima and vascular calcification after an injury. Model mice lacking COMP developed spontaneous dilated cardiomyopathy and heart failure. Evidence revealed that COMP is an endogenous thrombin inhibitor and negatively modulates hemostasis and thrombosis.

Target Name cartilage oligomeric matrix protein
Alternative Names CompCartilage oligomeric matrix protein ELISA kit; COMP ELISA kit
Abbreviation COMP
Uniprot No. P35444
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/ml - 5000 pg/ml
Sensitivity 19.5 pg/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Precision  
Linearity  

Recovery   

Typical Data  
Materials provided
  • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat COMP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled COMP antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
Gene References into Functions
  1. COMP could normally have a protective role against PASMC phenotype switching PMID: 28860005
  2. these findings revealed the essential role of COMP in retarding the development of vascular aging and vascular smooth muscle cell senescence. PMID: 27498005
  3. COMP-Ang1-stimulated proliferation, differentiation, and migration of progenitor cells may involve the Tie-2-mediated activation of p38 MAPK and PI3K/Akt pathways PMID: 25446117
  4. results suggest the potential importance of COMP-Ang1 as a new therapy for impaired bone formation that is associated with diabetes and advanced age PMID: 23850469
  5. The presence of cleaved apoptosis inducing factor (tAIF) and the absence of activated caspases indicated that retention of D469del-COMP triggers cell death in chondrocytes by necroptosis, a caspase-independent programmed necrosis. PMID: 22154936
  6. Cartilage oligomeric matrix protein is a novel inhibitor of vascular smooth muscle calcification. PMID: 21350216
  7. Overexpression of COMP markedly suppressed PDGF-BB-elicited VSMC dedifferentiation. Alpha(7)beta(1) integrin is a potential receptor for COMP. PMID: 20019333
  8. Matrix-matrix interaction of cartilage oligomeric matrix protein and fibronectin. PMID: 12225811
  9. The X-ray structure of the COMP coiled coil domain in complex with vitamin D(3) at 1.7 A resolution is reported. COMP exerts a distinct mechanism of interaction mainly defined by the pattern of hydrophobic core residues. PMID: 12426368
  10. COMP has a role in development of the extracellular matrix and in a cell culture model for pseudoachondroplasia PMID: 15579310
  11. COMP secretion is affected by the signal peptide PMID: 15749701
  12. Expression of COMP in both anulus fibrosus(AF) and nucleus pulposus(NP) of intervertebral disc(IVD). COMP is component of extracellular matrix of AF and NP, with lamellar distribution in AF. May play role in normal structure of IVD. PMID: 16778685
  13. ultrastructural distribution of COMP & TSP-4 & collagen fibril thickness in achilles tendon related to exercise; COMP immunolabeling decreased with longer running distance & was lower in pericellular & interterritorial compartments of high training rats PMID: 17882701
  14. To identify residues critical for the structure, stability, oligomerization and binding to vitamin D as well as two small molecules, all-trans-retinol and curcumin, an alanine-scanning mutagenesis study of cartilage oligomeric matrix protein is performed. PMID: 19681593

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Subcellular Location Secreted, extracellular space, extracellular matrix.
Protein Families Thrombospondin family
Tissue Specificity Expressed in differentiated and de-differentiated vascular smooth muscle cells (VSMCs) (at protein level).
Database Links

KEGG: rno:25304

STRING: 10116.ENSRNOP00000067037

UniGene: Rn.10343

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