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Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Citations (5) Protocol
Code CSB-E07443r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat NAG ELISA Kit was designed for the quantitative measurement of Rat NAG protein in serum, plasma, cell culture supernates, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL .
Target Name N-acetyl-β-D-glucosaminidase,NAG
Alternative Names Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
Abbreviation NAG
Uniprot No. Q6AXR4
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 1.56 mIU/mL-100 mIU/mL
Sensitivity 0.39 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %95
Range %90-100
1:2Average %96
Range %91-102
1:4Average %87
Range %84-95
1:8Average %95
Range %92-101
Recovery
The recovery of rat NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10095-105
EDTA plasma (n=4)9690-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
1002.495 2.471 2.483 2.306
501.955 1.987 1.971 1.794
251.356 1.368 1.362 1.185
12.50.934 0.963 0.949 0.772
6.250.587 0.597 0.592 0.415
3.120.425 0.451 0.438 0.261
1.560.361 0.374 0.368 0.191
00.175 0.178 0.177 　
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Hexb Proteins

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Target Background

Function
(From Uniprot)
Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
Subcellular Location Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
Protein Families Glycosyl hydrolase 20 family
Database Links

KEGG: rno:294673

UniGene: Rn.203067
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