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| Code | CSB-E08723m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 80-95 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 85-90 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| The recovery of mouse MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-95 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The mouse MPO ELISA Kit is engineered for accurate measurement of mouse MPO levels from samples including serum, plasma, cell culture supernates, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the MPO content in the sample. The color intensity is positively correlated with MPO content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
MPO is a key component of the innate immune system and is mainly released by neutrophils to defend against invading pathogens. MPO catalyzes the reaction of H2O2 with chloride ions (Cl−) to form hypochlorous acid (HOCl), which facilitates the destruction of microbes contained within the phagolysosome. MPO, together with its oxidative products, interacts with many lipids, proteins, and nucleic acids leading to some harmful effects in host tissues that are commonly related to ongoing inflammatory states such as atherosclerosis. MPO is ubiquitously distributed in atherosclerotic lesions and contributes to the initiation and progression of the disease mainly by oxidizing low-density lipoprotein (LDL) particles.
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