Mouse myeloperoxidase,MPO ELISA Kit

Instructions
Code CSB-E08723m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name myeloperoxidase
Alternative Names Mpo ELISA Kit; Myeloperoxidase ELISA Kit; MPO ELISA Kit; EC 1.11.2.2) [Cleaved into: Myeloperoxidase light chain; Myeloperoxidase heavy chain] ELISA Kit
Abbreviation MPO
Uniprot No. P11247
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %98
Range %92-104
1:200Average %87
Range %80-95
1:400Average %87
Range %85-90
1:800Average %95
Range %89-101
Recovery
The recovery of mouse MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8882-95
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.293 2.233 2.263 2.212
10001.386 1.275 1.331 1.280
5000.772 0.752 0.762 0.711
2500.496 0.491 0.494 0.443
1250.282 0.264 0.273 0.222
62.50.222 0.205 0.214 0.163
31.20.102 0.100 0.101 0.050
00.053 0.049 0.051  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Gene References into Functions
  1. Severe clinical and pathological disease is associated with the induction of anti-MPO IgG/LPS-mediated glomerulonephritis. PMID: 28186568
  2. These studies further support involvement of MPO-catalyzed oxidative processes in both the development of atherosclerosis and diabetes risk PMID: 28069522
  3. Neutrophils are abundant in abdominal aortic aneurysm (AAA), and myeloperoxidase (MPO), prominently expressed in neutrophils, is associated with AAA. taurine, which can scavenge MPO-generated oxidants, can prevent AAA formation. PMID: 28971841
  4. PTX3 attenuates the production of murine myeloperoxidase-anti-neutrophil cytoplasmic antibody induced by aluminum salt adjuvant. PMID: 28850023
  5. There is a smaller number of MPO positive neutrophils in the inflammatory infiltrate of a resistant model of amoebic liver abscess. PMID: 28796788
  6. thisa study shows that neutrophil myeloperoxidase plays a paradoxical role in bacterial clearance and tissue damage in pneumococcal acute otitis media PMID: 28359218
  7. lung MPO activity increased following induction of sepsis with CLP while siRNA treatment significantly reduced MPO activity. Liver and lung cytokine and chemokine levels in CLP-induced sepsis reduced following treatment with siRNA. These findings show a crucial pro-inflammatory role for H2S synthesized by CSE in macrophages in sepsis and suggest CSE gene silencing with siRNA as a potential therapeutic approach for this co PMID: 26949091
  8. MPO deficiency upregulates the expression of several proinflammatory cytokines and chemokines in mouse neutrophils PMID: 26573963
  9. Myeloperoxidase may be an important determinant of diet and inflammation on colon cancer risk via its effect on endogenous exposure to oxidants and acrolein. PMID: 26262998
  10. Alkalinity of neutrophil phagocytic vacuoles is modulated by HVCN1 and has consequences for myeloperoxidase activity. PMID: 25885273
  11. ethanol catabolism in renal tubules results in a self-perpetuating cycle of CYP2E1 induction, local PTAFR ligand formation, and neutrophil infiltration and activation that leads to myeloperoxidase-dependent oxidation and damage to kidney function. PMID: 26003521
  12. These findings suggest that myeloperoxidase may contribute importantly to formation and rupture of CA. PMID: 25922506
  13. Myeloperoxidase binds to RBC membranes in vitro and in vivo, is transported by RBCs to remote sites, and affects endothelial function as well as systemic vascular resistance. PMID: 24976018
  14. MPO knockout mice were protected from high fat diet-enhanced body weight gain and insulin resistance. Lack of MPO also attenuated HFD-induced macrophage infiltration and expression of proinflammatory cytokines. PMID: 25024373
  15. these results demonstrate that MPO deficiency ameliorates renal injury in the renal ablation model of chronic kidney disease in mice. PMID: 24990898
  16. MPO contributes to the development of arthritis despite suppressing adaptive immunity in secondary lymphoid organs. This suggests distinct effects of local MPO on arthritogenic effector responses. PMID: 24757143
  17. data suggest that the conversion of exogenous HE to 2-Cl-E(+) may be a useful selective and sensitive marker for MPO activity in addition to 3-Cl-Tyr. PMID: 24436331
  18. the role of leukocyte activation, leukocyte-derived MPO and MPO-generated oxidants on BBB function PMID: 23691142
  19. Data suggest that expression of Mpo in kidney is significantly correlated with severity of kidney damage in a mouse model of type 2 diabetes. PMID: 23268804
  20. Neu-164 and Neu-107, two novel antioxidant and anti-myeloperoxidase compounds, inhibit acute cigarette smoke-induced lung inflammation. PMID: 23686858
  21. Data indicate that myeloperoxidase-specific anti-neutrophil cytoplasmic antibodies (MPO-ANCA) induces injury via both humoral and cell mediated immune mechanisms. PMID: 23665205
  22. The role of genetics in myeloperoxidase -antineutrophil cytoplasmic autoantibodies necrotizing and crescentic glomerulonephritis severity was investigated using 13 inbred mouse strains. PMID: 23384999
  23. MPO, via its catalytic activity, inhibits the generation of adaptive immunity by suppressing dendritic cell activation, antigen uptake/processing, and migration to lymph nodes to limit pathological tissue inflammation. PMID: 23509155
  24. ceruloplasmin should provide a protective shield against inadvertent oxidant production by myeloperoxidase during inflammation PMID: 23306200
  25. The immunodominant myeloperoxidase T-cell epitope induces local cell-mediated injury in antimyeloperoxidase glomerulonephritis. PMID: 22955884
  26. MPO exacerbated secondary injury and impaired the functional recovery by generating strong oxidant HOCl, and enhancing neutrophil infiltration after spinal cord injury. PMID: 22322369
  27. Results provide evidence for the participation of myeloperoxidase (MPO) - one of the key-orchestrators of inflammatory response. PMID: 22479306
  28. The present study suggests that myeloperoxidase-mediated OCl(-) generation affects claudin molecules and leads to protein leakage and viral spread as a damage factor in influenza-induced acute respiratory distress syndrome. PMID: 22211924
  29. Green tea extract attenuates NADPH oxidase activity and the expression of myeloperoxidase and inducible nitric oxide synthase in ob/ob mice, thereby suppressing oxidative and nitrative modifications that otherwise lead to liver injury. PMID: 21543212
  30. MPO has dual functionality under rotenone-exposed conditions, playing a critical regulatory role in modulating pathological and protective events in the brain PMID: 21704008
  31. MPO is susceptible to the free radicals it generates, and this Achilles' heel of the enzyme can be exploited to block oxidative stress during inflammation. PMID: 21880720
  32. Rosiglitazone inhibits MPO secretion in RAW264.7 cells. PMID: 19778788
  33. Given MPO's affinity to both the endothelial and the leukocyte's surface, MPO evolves as a mediator of PMN recruitment because of its positive surface charge. PMID: 20980678
  34. Data indicate that myeloperoxidase contributes to the reduced reverse cholesterol transport observed during acute phase responses. PMID: 20061576
  35. myeloperoxidase is responsible for protein nitration and cardiomyocyte apoptosis in nonlethal traumatic mice. PMID: 20153732
  36. Contribution of myeloperoxidase to coronary artery vasculitis associated with MPO-ANCA production. PMID: 11831441
  37. MPO and NADPH-oxidase are equally important for early host defense against a large inoculum of Candida. PMID: 12085336
  38. MPO was observed to modulate the vascular signaling and vasodilatory functions of nitric oxide (NO) during acute inflammation PMID: 12089442
  39. Data show that myeloperoxidase and plasminogen activator inhibitor 1 play a critical role in the left ventricular response immediately after myocardial infarction. PMID: 12615902
  40. the MPO/H2O2 system has a role in up-regulating the catalytic activity of iNOS that occurs at sites of inflammation PMID: 14657339
  41. In knockout mice, suppressed MPO-derived oxidative/nitrative stress is associated with enhanced lung inflammation and persistent alveolar epithelial injury. PMID: 15020295
  42. Role of MPO in cellular immunity to fungi and bacteria in MPO knockout and chronic granulomatous disease mice. PMID: 15507755
  43. Compelling evidence is provided that susceptibility to infection with Gram-negative Klebsiella pneumoniae is enhanced in myeloperoxidase-deficient mice. PMID: 15661916
  44. mechanical loading activates neutrophil-mediated lysis of muscle cells through an MPO-dependent pathway PMID: 15790660
  45. aberrant MPO-antineutrophil cytoplasmic autoantibody production was exclusively controlled by Man-1 PMID: 16517735
  46. data suggest a major role of myeloperoxidase in the immunological defence to Cryptococcus neoformans infection PMID: 16914663
  47. myeloperoxidase is regulated by LXR and PPARalpha ligands PMID: 16956579
  48. Along with the activation of AMPD3, ischemia-reperfusion-induced lung inflammation is associated with increased MPO activity and TNF-alpha level. PMID: 17384464
  49. These results are consistent with the notion that nitric oxide and MPO contribute in liver tissue lipid peroxidation in peritonitis. PMID: 17901846
  50. blood myeloperoxidase is increased and myeloperoxidase-positive neutrophils develop in atherosclerotic lesions in LDLR-/- mice PMID: 17991873
  51. Taken together, these results demonstrated an important contribution of MPO in the induction of organ damage after renal I/R by influencing critical factors such as neutrophil extravasation but not complement activation. PMID: 18055546
  52. the lack of HOCl-mediated bacterial killing, and the absence of MPO-derived oxidants mitigates E. coli sepsis-induced lung inflammation and injury. PMID: 18424617
  53. Myeloperoxidase functions as a survival signal for neutrophils and thereby contribute to prolongation of inflammation. PMID: 18617697
  54. Estimation of MPO activity is a reliable indicator of inflammation PMID: 18619953
  55. The myeloperoxidase content of aortic tissue was significantly higher in all groups infected with E. coli PMID: 19221870
  56. MPO promotes the development of lung neutrophilia and indirectly influences subsequent chemokine and cytokine production by other cell types in the lung PMID: 19494324

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Subcellular Location Lysosome
Protein Families Peroxidase family, XPO subfamily
Database Links

KEGG: mmu:17523

STRING: 10090.ENSMUSP00000020779

UniGene: Mm.4668

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