Rat Catalase (CAT) ELISA kit

Instructions
Code CSB-E13439r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name catalase
Alternative Names Cat ELISA kit; Cas1Catalase ELISA kit; EC 1.11.1.6 ELISA kit
Abbreviation CAT
Uniprot No. P04762
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates
Detection Range 93.75 mIU/mL-6000 mIU/mL
Sensitivity 23.43 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 98  
Range % 94-102  
1:10 Average % 94  
Range % 90-97  
1:20 Average % 105  
Range % 98-109  
1:40 Average % 88  
Range % 82-94  
Recovery
The recovery of rat CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 82-94  
EDTA plasma (n=4) 92 87-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected  
6000 2.703 2.602 2.653 2.537  
3000 1.962 1.861 1.912 1.796  
1500 1.218 1.197 1.208 1.092  
750 0.734 0.723 0.729 0.613  
375 0.387 0.398 0.393 0.277  
187.5 0.297 0.286 0.292 0.176  
93.75 0.188 0.184 0.186 0.070  
0 0.115 0.117 0.116    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.
Gene References into Functions
  1. These results indicate that stress conditions most probably altered the cell redox equilibrium, thus influencing the antioxidant response in brain cortex. PMID: 29847079
  2. fenofibrate almost completely abolished GM-induced reactive oxygen species generation, which seemed to be mediated at least in part by the restoration of the expression of PPARalphadependent antioxidant enzymes, including catalase and superoxide dismutase (SOD)-1. PMID: 28290603
  3. These results indicate that CAT expression is significantly decreased in the hippocampi of aged animals and decreased CAT expression may be closely associated with aging. PMID: 27221506
  4. Catalase promoter region (-185 to +52) that contains binding sites for C/EBP-beta showed an augmentation in the methylation level along with a change in methylation pattern of CpG islands in response to PTU treatment PMID: 26459835
  5. Data show that nuclear factor erythroid 2-related factor (Nrf2), superoxide dismutase Sod1 and catalase antioxidant systems are downregulated in isolated arteries and vascular smooth muscle cells from stroke-prone spontaneously hypertensive rats (SHRSP). PMID: 26503970
  6. mitochondria-targeted antioxidant SkQ1 (50 nmol/kg during 5 days) significantly increased the mRNA levels of Nrf2 transcription factor and Nrf2-induced genes encoding antioxidant enzymes SOD1, SOD2, CAT, and GPx4 in rat peripheral blood leukocytes. PMID: 26071777
  7. Targeted mass spectrometry for the analysis of nutritive modulation of catalase and heme oxygenase-1 expression. PMID: 25639505
  8. Decrease in Sirtuin1 (SIRT1) and nuclear factor erythroid 2-related factor (Nrf2) and increase in nuclear factor kappa B (NFkappaB) gene expression in diabetes were associated with a decrease in CAT and GPx mRNA expression. PMID: 24740756
  9. Coronary resistance regulation seems to be more dependent on the hydrogen peroxide that is maintained at low levels by increased catalase activity. PMID: 24669281
  10. Catalase was found to participate in lead-induced alcohol intake and is elevated in blood following lead exposure. PMID: 23808586
  11. The marked increased in alcohol intake that follows an alcohol deprivation period is mediated by acetaldehyde generated in the brain by the action of catalase. PMID: 23527889
  12. Results suggest that the effects of estrogen on the cell-mediated immune responses are mediated through specific estrogen receptors alpha and beta and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). PMID: 23911387
  13. The content of catalase protein in mitochondrial extract increased with aging. PMID: 22683338
  14. Ornithine alpha-ketoglutarate modulates the levels of antioxidants glutathione peroxidase, superoxide dismutase and catalase, as well as lipid peroxidation products in ammonium acetate treated rats PMID: 22905402
  15. The increased expression of antioxidant catalase, specifically in hippocampal neurons, will provide protection from age-related cognitive decline. PMID: 21942371
  16. Ang II inhibited catalase (CAT) and peroxisome proliferator-activated receptor gamma (PPAR gamma) protein and mRNA expressions. PMID: 21295034
  17. Chronic psychosocial isolation attenuated peroxide-metabolizing enzymes, CAT and GPx, and decreased GLR activity. PMID: 20063054
  18. catalase activities in liver and kidney of diabetic male or female rats were significantly lower than in non-diabetic rats but the reduction was more pronounced in diabetic female rats. PMID: 20830981
  19. This protein has been found differentially expressed in the lumbar spinal cord from rats submitted to peripheral lesion during neonatal period. PMID: 20387083
  20. endogenous PGC-1 alpha is a negative regulator of vascular hypertrophy by up-regulating catalase expression and thus reducing reactive oxygen species levels PMID: 19940161
  21. No significant differences in superoxide dismutase and catalase activities, but MDA was decreased by ghrelin on day 10. Evidence for antioxidant properties of ghrelin in rat testis. PMID: 19891630
  22. in addition to transrepression of proinflammatory transcription factors, peroxisome proliferator-activated receptor-gamma may also be modulating catalase expression PMID: 12456800
  23. Levels of Cat mRNA were significantly reduced in congestive heart failure following myocardial infarction. PMID: 14575298
  24. Catalase activities decreased in the liver and heart of rats with hypothyroidism PMID: 14624470
  25. Over-expression catalase genes in primary hippocampal or cortical cultures resulted in increased enzymatic activity of the cognate protein. PMID: 14713308
  26. Changes in post-brain injury/intervention levels of NGF and activity of three major enzymes responsible for ROS scavenging, catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), was determined in the hippocampus PMID: 15287906
  27. Medroxyprogesterone acetate and idarubicin both inhibit blood levels of this enzyme PMID: 15372991
  28. generation of H(2)O(2) and expression of CAT are coordinated and indicative of epidermal differentiation as well as of the role of CAT in repairing redox damage PMID: 15734284
  29. influence of gender upon activities of catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD) in hypertension PMID: 15773229
  30. catalase and glutathione peroxidase are downregulated in renal insufficiency PMID: 15777843
  31. rats subjected to ovariectomy presented a significant increase in brain Na+, K+-ATPase, AChE and CAT activities, but did not change the oxidative stress parameters PMID: 15869839
  32. Multiple complex formation of C/EBP-beta regulates transcription in the TATA-less catalase promoter. PMID: 16223488
  33. psychological stressor induces a "priming" effect specifically on the CuZnSOD and CAT activity PMID: 16238459
  34. Results suggest that amphetamine exposure is associated with an imbalance between superoxide dismutase and catalase activity in the prefrontal cortex, hippocampus and striatum. PMID: 16770742
  35. Ischemic attack causes a rapid response in hippocampal tissue as well as in the cerebrospinal fluid, represented by an increase in the activity of endogenous antioxidant enzymes SOD and CAT PMID: 17215005
  36. catalase is a major participant in the defense of 36B10 glioma cells against oxidative stress PMID: 17320761
  37. mRNA and protein expressions of superoxide dismutase (SOD) and catalase increase after ischemia (I) and reperfusion (R) of the rat liver. PMID: 17524832
  38. Nimesulide decreased Cat activity significantly in rats subjected to ischemia. PMID: 17564305
  39. observations are compatible with the presence of endogenous heme-containing catalase with an activity of 825 +/- 15 units, which contributes to mitochondrial protection against endogenous or exogenous H2O2 PMID: 17576767
  40. Findings strongly support the assumption that catalase plays a significant role in acetaldehyde formation directly in the rat brain. PMID: 17597213
  41. Even though mRNA expressions of both catalase and Cu-Zn SOD were not changed, the protein levels increased in parallel to activities in the case of another antioxidant, alpha-lipoic acid. PMID: 18008141
  42. Results describe the activity of superoxide dismutase and catalase at different ages following methylphenidate treatment in young rats. PMID: 18049893
  43. Elevated catalase levels in hearts of long-term type 1 diabetic rats signals improved recovery of postischemic cardiac function and reduced reperfusion arrhythmia. PMID: 18430061
  44. Reduced availability of reactive oxygen species by catalase is a key component of neuroprotection against various toxic stimuli PMID: 19302986

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Subcellular Location Peroxisome
Protein Families Catalase family
Database Links

KEGG: rno:24248

STRING: 10116.ENSRNOP00000011230

UniGene: Rn.3001

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