Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
| Code | CSB-E13439r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 90-97 | |||||
| 1:20 | Average % | 105 | ||||
| Range % | 98-109 | |||||
| 1:40 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| The recovery of rat CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 82-94 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The product CSB-E13439r is a ready-to-use microwell, strip plate ELISA kit for quantitatively analyzing the presence of the Catalase (CAT) in different biological samples, including serum, plasma, and tissue homogenates. This CAT ELISA kit specifically recognizes the rat CAT in the sample. It adopts the Sandwich ELISA technique in conjunction with antibody-antigen specific binding and an enzyme colorimetric reaction to indicate the presence of rat CAT and quantitatively measure the concentration of CAT in the samples.
Catalase is an antioxidant enzyme present in almost all aerobic organisms and is responsible for breaking down cellular hydrogen peroxide to generate water and oxygen thus attenuating oxidative stress. Deficiency or dysregulation of catalase is associated with the pathogenesis of various age-related degenerative disorders such as hypertension, diabetes, anemis, vitiligo, and cancer. An anomaly of catalase activity is inherited in acatalasemia, a rare genetic disease. A decrease in catalase activity in tumor patients is more likely to be due to reduced enzyme synthesis rather than to catalase mutations.
There are currently no reviews for this product.
