Recombinant Human GTP-binding nuclear protein Ran(RAN)

Code CSB-EP019307HU
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names RAN
Uniprot No. P62826
Research Area Cell Biology
Alternative Names Androgen receptor associated protein 24; Androgen receptor-associated protein 24; ARA 24; ARA24; Gsp1; GTP binding nuclear protein RAN; GTP-binding nuclear protein Ran; GTPase Ran; Guanosine triphosphatase Ran; LPS; OK/SW-cl.81; ran; RAN member RAS oncogene family; RAN_HUMAN; RanGTPase; Ras like protein TC4; Ras related nuclear protein; Ras-like protein TC4; Ras-related nuclear protein; RASL2 8; TC 4; TC4
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-216aa
Target Protein Sequence AAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 51.3kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensure the directionality of the transport.
Gene References into Functions
  1. DICER rs3742330 AG+GG genotype was associated with more advanced T stage compared to AA genotype ( P=0.009). More patients with XPO5 rs2257082 CC genotype had poorly differentiated tumors compared with CT+TT genotype carriers..), carriers of RAN rs14035 CC genotype had higher three-year OS rate than carriers of CT+TT genotype (adjusted HR 3.174; 95% CI 1.010, 9.973; P=0.048). PMID: 29683064
  2. Taken together, the results provide strong in vitro evidence of the involvement of Ran in the progression of breast cancer and suggest that it could have high potential as a therapeutic target and/or marker of disease. PMID: 29750309
  3. Molecular structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A has been reported. PMID: 27306458
  4. The mobility of Emerin depends on RanGTP. In cells overexpressing YFP-Emerin, the mobility of YFP-Emerin was higher in Samp1 knock out cells and lower in cells overexpressing Samp1, suggesting that Samp1 significantly attenuates the mobility of Emerin in the nuclear envelope.The affinity between Samp1 and Emerin is decreased in the presence of Ran, suggesting that Ran attenuates the interaction between Samp1 and Emerin. PMID: 29510091
  5. KPNB1 and Ran protein jointly mediated the nuclear import of NDV M protein, showing that KPNB1 protein interacted with NDV M protein to form binary complex and then entered into the nucleus with the assistance of Ran protein. PMID: 29746765
  6. Ran GTPase promotes cancer progression via Met recepto-rmediated downstream signaling PMID: 27716616
  7. Data show that the distribution of components of the ras-related nuclear protein (Ran) pathway that influence microtubule behaviors is determined by their interactions with microtubules, resulting in microtubule nucleators being localized by the microtubules whose formation they stimulate. PMID: 27439876
  8. The RAS-related nuclear protein ((P) ran), breast cancer metastasis suppressor 1 ((P) brms1) and minichromosome maintenance complex component 5 ((P) mcm5) promoters have the specificity and strength needed for cancer-specific expression-targeted gene therapy. PMID: 27140445
  9. Authors found that decreasing Ran-GTP levels or tethering active Ran to the equatorial membrane affects anillin's localization and causes cytokinesis phenotypes. PMID: 28931593
  10. lysine-acetylation regulates nearly all aspects of Ran-function such as RCC1 catalyzed nucleotide exchange, intrinsic nucleotide hydrolysis, its interaction with NTF2 and the formation of import- and export-complexes. PMID: 26507377
  11. These results showed that BLM enters the nucleus via the importin beta1, RanGDP and NTF2 dependent pathway, demonstrating for the first time the nuclear trafficking mechanism of a DNA helicase. PMID: 29017749
  12. RAN Translation Regulated by Muscleblind Proteins in Myotonic Dystrophy Type 2 PMID: 28910618
  13. we demonstrated that DICER (rs3742330) and RAN (rs14035) were associated with the survival of HCC patients PMID: 27611467
  14. RAN translation from antisense CCG repeats generates novel proteins that accumulate in ubiquitinated inclusions in Fragile X-associated tremor/ataxia syndrome patients. PMID: 27761921
  15. interaction of Samp1 and Ran in the inner nuclear membrane PMID: 27541860
  16. RAN role in cell cycle, DNA repair and DNA damage-induced cell senescence PMID: 26864624
  17. Binding of Ran to NTF2 is required for NTF2 to inhibit nuclear expansion and import of large cargo molecules. PMID: 26823604
  18. RAN is pivotal in linking spatiotemporal control of centrosome regulators to the cell cycle machinery. PMID: 26725228
  19. FGF2 nuclear translocation is regulated by Karyopherin-beta2 and Ran GTPase in human glioblastoma cells PMID: 26056081
  20. Enterovirus 71-induced downregulation of miR-197 expression increased the expression of RAN, which supported the nuclear transport of the essential viral proteins 3D/3CD and host protein hnRNP K for viral replication. PMID: 26581983
  21. Inter-cellular transport of ran GTPase PMID: 25894517
  22. Variations in RAN rs14035of Korean patients are significantly associated with their risk of colorectal cancer. PMID: 26147304
  23. Data show that RNA-binding protein LIN28B coordinates the expression of the oncogene RAN protein and aurora A kinase (AURKA) in neuroblastoma. PMID: 26481147
  24. Elevated metaphase RanGTP levels use Ubr5 to couple overall chromosome congression to SAC silencing. PMID: 26438829
  25. Data show that importin-beta (impbeta) alters the nuclear pore's permeability in a Ran-dependent manner, suggesting that impbeta is a functional component of the nuclear pore complex (NPC). PMID: 25748139
  26. RAN nucleo-cytoplasmic transport and mitotic spindle assembly partners XPO7 and TPX2 have roles in serous epithelial ovarian cancer PMID: 24625450
  27. comparison of the GTPase reaction of the slower hydrolyzing GTPase Ran with Ras PMID: 26272610
  28. Ran silencing did not affect the A375 invasive capability, while it was enhanced in 526 cells, suggesting that Ran knockdown, by AurkA down-regulation, resulted in a Ran-independent enhanced melanoma cell invasion. PMID: 25444926
  29. stress can disrupt the Ran gradients through RCC1-dependent and RCC1-independent mechanisms PMID: 25452301
  30. based on the new structures suggests LM phosphorylation status may mediate Ran's selection of exportin(s) and cargo(s), perverting these native trafficking elements into the lethal antihost Nup phosphorylation pathways. PMID: 25331866
  31. PI3Kbeta regulates the nuclear envelope through upstream regulation of RCC1 and Ran PMID: 25348717
  32. Towards understanding its molecular mechanism, a 2.9 A resolution crystal structure of human TRN-SR2 complexed with the small GTPase Ran has been determined PMID: 24915079
  33. Association between recurrent pregnancy loss development and the polymorphism of the miRNA machinery gene RAN and combined genotype of DROSHA/DICER. PMID: 24769857
  34. reducing the nuclear concentration of Ran is sufficient to induce reactive oxygen species irrespective of progerin. PMID: 24523287
  35. Our findings emphasize the important role of Ran in differentiation, disease stage, and metastasis in human colorectal cancer PMID: 22956174
  36. our data suggest a model for mitotic spindle positioning in which RanGTP and CLASP1 cooperate to align the spindle along the long axis of the dividing cell. PMID: 23783028
  37. Thioredoxin-like protein 2 is overexpressed in colon cancer and promotes cancer cell metastasis by interaction with ran. PMID: 23311631
  38. MiR-203 may act as novel tumor suppressor in esophageal cancer through down-regulating the expression of Ran and miR-21. PMID: 24001611
  39. Polymorphism in RAN gene is associated with HBV-related hepatocellular carcinoma PMID: 23868705
  40. a model wherein one monomer of TRN-SR2 is bound to one monomer of RanGTP. PMID: 23878195
  41. Overexpressed Ran in pancreatic cancer tissues was found highly correlated with the histological grade. Downregulation of Ran led to significant suppression of cell proliferation, cell cycle arrest at the G1/S phase and induction of apoptosis. PMID: 24076388
  42. Data show that five genes CKAP5, KPNB1, RAN, TPX2 and KIF11 were shown to be essential for tumor cell survival in both head and neck squamous cell carcinoma (HNSCC)and non-small cell lung cancer (NSCLC), but most particularly in HNSCC. PMID: 23444224
  43. Therefore, the Encephalomyocarditis virus leader protein:Ran GTPase binding, mediated by the linker-hinge, is a required step in the leader protein-induced nuclear transport inhibition. PMID: 23711384
  44. RCC1 facilitates a tight binding between the encephalomyocarditis virus leader and cellular Ran GTPase. PMID: 23536659
  45. Reduced RAN expression and disrupted transport between cytoplasm and nucleus are the key events in Alzheimer's disease pathophysiology. PMID: 23308199
  46. Reduction in Ran levels causes cytoplasmic decrease and nuclear accumulation of importin alpha leading to cellular senescence in normal cells. PMID: 23160023
  47. Results suggest that Ran is required for and is a potential therapeutic target of Myc-driven cancer progression in both breast and lung cancers. PMID: 23468463
  48. RanBPM loss of expression results in constitutive activation of the ERK pathway and promotes cellular events leading to cellular transformation and tumorigenesis. PMID: 23118896
  49. The increased expression of Ran in lung squamous cell carcinoma might be correlated with carcinogenesis. PMID: 21033436
  50. a novel connection between the hyper-activation of the small GTPase Ran and the matricellular protein SMOC-2 that has important consequences for oncogenic transformation. PMID: 22679017
  51. Overexpression of Ran in clinical specimens is significantly associated with poor patient outcome in both breast and lung cancers. PMID: 22090358
  52. Loss of ran GTP-Binding Protein is associated with overt diabetes, with hyperglycemia, reduced insulin production, and nearly complete loss of islet number and islet mass. PMID: 22114719
  53. Ran GTP plays key role in ciliogenesis and ciliary protein transport. PMID: 21998203
  54. MCRS1 is a RanGTP-regulated factor essential for non-centrosomal microtubule assembly. PMID: 22081094
  55. in vitro and in vivo phosphorylation of RanBP1 by Plk1 as well as the importance of phosphorylation of RanBP1 in the interaction between Plk1 and Ran during early mitosis, is demonstrated. PMID: 21813642
  56. RanGTP may play an important role in specifying that spindle assembly occurs around chromosomes PMID: 21692748
  57. the cellular effects of progerin expression in Hutchinson-Gilford progeria syndrome are transduced, at least in part, through reduced function of the Ran GTPase and E2 SUMOylation pathways PMID: 21670151
  58. study defines importin-alpha/importin-beta1/Ran as the molecular mechanism by which STAT3 traffics to the nucleus PMID: 21625522
  59. Crystal structure of the Ran-binding domain (RBD) from RanBP3 and compare it to RBD structures from RanBP1 and RanBP2 in complex with Ran and CRM1. PMID: 21364925
  60. [review] The cellular functions of Ran are mediated by RanGTP interactions with nuclear transport receptors related to importin beta and depend on the existence of chromosome-centered RanGTP gradient. PMID: 21630149
  61. Data suggest actin accumulation in senescent cells is due to the failure of RanGTP restoration with ATP deficiency and NTF2 accumulation, resulting in decreased actin export via Exp6 inactivation. PMID: 21195711
  62. Downregulation of the Ran GTPase effector RanBP1 is required for nuclear reorganisation. PMID: 20658144
  63. Data show that downregulation of Ran expression in aggressive ovarian cancer cell lines affects cellular proliferation by inducing a caspase-3 associated apoptosis. PMID: 20942967
  64. Ran-GTP and Epac1 interact with each other in vivo and in vitro. This binding requires a previously uncharacterized Ras association domain in Epac1. PMID: 20547757
  65. Activation of the Ran GTPase is subject to growth factor regulation and can give rise to cellular transformation. PMID: 20028979
  66. Data show that ARHI could compete for Ran-importin binding and induce disruption of importin-binding to cargo proteins, including STAT3. PMID: 19435463
  67. Data show that Nup107-160 promotes spindle assembly through Ran-GTP-regulated nucleation of microtubules by gamma-TuRC at kinetochores, and reveal a relationship between nucleoporins and the microtubule cytoskeleton. PMID: 20081840
  68. the small GTPase RAN is a novel MAD2B binding protein PMID: 19753112
  69. GTP-binding proteins G(salpha), G(ialpha), and Ran identified in mitochondria of human placenta PMID: 11779226
  70. C terminus of Ran is a major determinant of the state of Ran, and that removal of this allows the GDP-bound form to adopt a GTP-like conformation, thereby creating a constitutively active protein. PMID: 12051861
  71. chromosomal interactions during meiosis and mitosis PMID: 12415012
  72. structure of the Ran-RanBP1-RanGAP complex PMID: 14585972
  73. Ran modulates at least two processes involved in spindle assembly PMID: 14600264
  74. data suggest that HPV16 E7 translocates through the nuclear pores via a nonclassical Ran-dependent pathway, independent of the main cytosolic Kap beta import receptors PMID: 14675621
  75. the cell-cycle machinery directly regulates the Ran-signaling pathway by placing a high RanGTP concentration on the mitotic chromosome; RCC1 to couples RanGTP production to chromosome binding PMID: 15014043
  76. nuclear import of the transcription factor PU.1 occurs via RanGTP-stimulated binding to Nup153 PMID: 15632149
  77. Keratin sequestration of oxidized Ran may provide a back-up protective mechanism in some cases of oxidative injury PMID: 15691838
  78. Review proposes that Ran/Crm1 may act as a 'loading dock' to coordinate various checkpoint factors in regulating the fidelity of centrosome duplication during cell cycle progression. PMID: 16294017
  79. Findings indicate that the decrease in ATP levels induced by cellular stress causes a decrease in RanGTP levels and a collapse of Ran distribution in the cytoplasmic and nuclear compartments. PMID: 16368437
  80. Phosphorylated wild type RanGAP1, but not a mutant harboring a mutation at the phosphorylation site 358S, efficiently formed a stable ternary complex with Ran and RanBP1 in vivo, suggesting that the 358S phosphorylation of RanGAP1 affects the Ran system. PMID: 16428860
  81. Exportin 4 is sufficient for carrying the in vitro nuclear export of Smad3 in cooperation with Ran. PMID: 16449645
  82. results demonstrate that conserved Ran-regulated pathways are involved in multiple, parallel processes required for spindle function, but that their relative contribution differs in chromatin- versus centrosome/kinetochore-driven spindle assembly systems PMID: 16572176
  83. High level of Ran appears to be more tightly associated with a poor prognosis. PMID: 16572426
  84. Plk1 regulates the spindle organization partially through its phosphorylation on Ran PMID: 16930555
  85. studies elucidate the Ran-binding interface on Nup153 and, more broadly, provide insight into the versatility of this zinc finger binding module PMID: 17426026
  86. developed a spatial model that allowed the simulation of RanGTP production with different degrees of chromosome alignment in mitosis PMID: 17671426
  87. Ran system is a target of hyperosmotic stress signaling, and cells use protein localization-based mechanisms as part of a rapid stress response PMID: 17761537
  88. A cell-free import assay showed that the nuclear transport of the human herpesvirus 6 U69 protein was mediated by importin alpha/beta in conjunction with the small GTPase Ran. PMID: 18003734
  89. High expression of Ran GTPase is associated with local invasion and metastasis of human clear cell renal cell carcinoma PMID: 18241036
  90. Results report that NTF2 and Ran control nuclear import of the filamentous actin capping protein CapG. PMID: 18266911
  91. Ran-GTP-mediated cargo release promotes the accumulation of hKid on chromosomes. PMID: 18268099
  92. The complex of RanGTP, the export receptor Crm1, and nuclear export signal-bearing proteins regulates microtubule nucleation at kinetochores. PMID: 18287525
  93. RanBP10 might serve as a molecular link between Ran and noncentrosomal microtubules PMID: 18347012
  94. These data demonstrate that ARA24/Ran increases AR transactivation by enhancing the androgen receptor N-C interaction in the nucleus. PMID: 18565325
  95. Survivin is a novel effector of Ran signaling, and this pathway may be preferentially exploited for spindle assembly in tumor cells. PMID: 18591255
  96. Ran is a novel negative regulator of nuclear VRK1 and VRK2 kinase activity, which may vary in different subcellular localizations generating an asymmetric intracellular distribution of kinase activity depending on local protein interactions. PMID: 18617507
  97. These results suggest that Ran suppresses paclitaxel-induced cell death through the downregulation of JNK-mediated signal pathways. PMID: 18690538
  98. Data show the crystal structure of the Kap95p-RanGDP complex shows that Kap95p induces the switch I and II regions of RanGDP to adopt a conformation that resembles that of the GTP-bound form. PMID: 18708071
  99. reduction of the affinity by RanGTP really occurs at the nucleoplasmic side of the entire nuclear pore complex PMID: 18845677
  100. Therefore, we propose that RCC1 reads the histone code created by caspase-activated Mst1 to initiate apoptosis by reducing the level of RanGTP in the nucleus. PMID: 19060893

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Subcellular Location Nucleus, Nucleus envelope, Cytoplasm, Melanosome
Protein Families Small GTPase superfamily, Ran family
Tissue Specificity Expressed in a variety of tissues.
Database Links

HGNC: 9846

OMIM: 601179

KEGG: hsa:5901

STRING: 9606.ENSP00000376176

UniGene: Hs.10842

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