Human tyrosine hydroxylase,TH ELISA Kit

Code CSB-E09661h
Size 96T,5×96T,10×96T
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Product Details

Target Name
tyrosine hydroxylase
Alternative Names
Dystonia 14 ELISA Kit; DYT14 ELISA Kit; DYT5b ELISA Kit; EC 1.14.16.2 ELISA Kit; OTTHUMP00000011225 ELISA Kit; OTTHUMP00000011226 ELISA Kit; ple ELISA Kit; Protein Pale ELISA Kit; TH ELISA Kit; The ELISA Kit; TY3H_HUMAN ELISA Kit; TYH ELISA Kit; Tyrosine 3 hydroxylase ELISA Kit; Tyrosine 3 monooxygenase ELISA Kit; Tyrosine 3-hydroxylase ELISA Kit; Tyrosine 3-monooxygenase ELISA Kit; Tyrosine hydroxylase ELISA Kit
Abbreviation
TH
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 87-103  
1:2 Average % 88  
Range % 82-97  
1:4 Average % 96  
Range % 85-105  
1:8 Average % 95  
Range % 89-110  
Recovery
The recovery of human TH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-96  
EDTA plasma (n=4) 93 85-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.539 2.489 2.514 2.398  
10 2.013 1.995 2.004 1.888  
5 1.497 1.542 1.520 1.404  
2.5 1.090 1.027 1.059 0.943  
1.25 0.676 0.661 0.669 0.553  
0.625 0.425 0.434 0.430 0.314  
0.312 0.251 0.263 0.257 0.141  
0 0.114 0.118 0.116    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human tyrosine hydroxylase (TH) ELISA Kit was designed for the quantitative measurement of Human tyrosine hydroxylase (TH) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Function
(From Uniprot)
Plays an important role in the physiology of adrenergic neurons. Positively regulates the regression of retinal hyaloid vessels during postnatal development.
Gene References into Functions
  1. These results provide a novel mechanism of how NO can modulate TH's enzymatic activity through S-nitrosylation. PMID: 28287127
  2. It is a genetic risk for Parkinson's disease. PMID: 29724574
  3. One novel mutation of c.679A>G (p.T227A) in GCH1 and 3 known mutations of c.457C>T (p.R153X), c.739G>A (p.G247S), and c.698G>A (p.R227H) in tyrosine hydroxylase (TH) have been found and predicted to be damaging or deleterious. PMID: 29405179
  4. This study does not support that early-onset PD may be the male presentation of TH deficiency attributed to this founder mutation in Greek patients. PMID: 27666733
  5. A novel heterozygous variant in tyrosine hydroxylase was identified in Chinese patients with dopa-responsive dystonia. PMID: 27619486
  6. This study indicates that mutations in TH are rare in late-onset Parkinson's disease. PMID: 27185167
  7. The purpose of this study is to investigate the clinical significance of tyrosine hydroxylase (TH) expression in peripheral blood (PB) at diagnosis in patients with neuroblastoma.. The treatment intensity should be tailored according to TH expression in PB at diagnosis. PMID: 27034145
  8. Our results suggest that the TH-immunoreactive cells in the human cortex do not overlap with any known neurochemically-defined subsets of interneurons and provide further evidence of differences in the phenotype of these cells across species. PMID: 27448941
  9. Results show that the positive rates and expression levels of nestin, tyrosine hydroxylase (TH), GFAP and IL-17 were significantly decreased while Foxp3 and the ratio of Foxp3/IL-17 were statistically elevated in BM of AML patients. PMID: 27016413
  10. Data suggest that TH phosphorylated at Ser-31 co-distributes with Golgi complexes and synaptic-like vesicles in rat and human dopaminergic neurons/cell lines; Ser-31 phosphorylation may regulate TH subcellular localization by enabling its transport along microtubules, notably toward the projection terminals. PMID: 28637871
  11. TH is a robust interaction partner of different 14-3-3 dimer types with moderate variability between the 14-3-3 dimers on their regulation of TH. PMID: 26825549
  12. Germline mutations in the TH gene are linked to Familial isolated pituitary adenoma in a Brazilian Family. PMID: 27245436
  13. No statistically significant differences were found between cases and controls for the allele frequencies in five genes: TH, SLC18A2, DRD1, DRD3 and COMT. Conversely, some alleles of the 12 sNPs from the DRD2 locus and the 5 from the MAOA locus showed significant associations with excessive alcohol consumption. PMID: 26447226
  14. Results show that metastasis-associated protein 1 (MTA1) and tyrosine hydroxylase (TH) levels were significantly down-regulated in Parkinson disease (PD) samples as compared with normal brain tissue PMID: 27044752
  15. the reduction of tyrosine hydroxylase-immunoreactive neurons occurring in the locus coeruleus after perinatal hypoxic insults persists into adulthood PMID: 26647061
  16. The data suggest that presence of a homozygous V81M polymorphism is associated with more severe freezing of gait in patients with Parkinson's disease PMID: 26732803
  17. In this study we found that TH protein levels did not differ between control and schizophrenia groups in the nucleus accumbens. PMID: 26386900
  18. In high-risk metastatic Neuroblastoma, TH and DCX mRNA quantification could be used for the assessment of response to treatment and for early detection of progressive disease or relapses. PMID: 26498952
  19. the allelic frequency of the TH01 marker in 171 Swiss sudden infant death syndrome (SIDS) infants and 500 healthy and gender-matched Caucasian adults showed that the 9.3 allele is similarly distributed in SIDS cases and controls (27.2% vs. 25.6%; p-value = 0.562). PMID: 24975687
  20. This study showed that a new tyrosine hydroxylase knock-in mouse model of l-DOPA-responsive dystonia. PMID: 26220941
  21. The mutant tyrosine hydroxylase enzyme was unstable and exhibited deficient stabilization by catecholamines, leading to decline of brain tyrosine hydroxylase-immunoreactivity in the Th knock-in mice. PMID: 26276013
  22. Thus, the hTH-GFP reporter rat should be a valuable tool for Parkinson's disease research. PMID: 25462571
  23. A detailed analysis of the interaction between singly or doubly phosphorylated human tyrosine hydroxylase isoform 1(1-50) peptides and 14-3-3zeta PMID: 25418103
  24. Study found evidence that DNA variation in the ADRA2A gene may be causally related to ADHD-like behaviors, and for a novel association between a TH gene variant and intra-individual variability PMID: 24166412
  25. Proteomics analysis show that Ser40 of TH protein does not significantly contribute to the binding of 14-3-3gamma, and rather has reduced accessibility in the TH:14-3-3gamma complex. PMID: 24947669
  26. increased expression of TH and GAP43 might be a molecular mechanism for left atrial myoelectricity remodeling of aging atrial fibrillation patients, which might be potential therapeutic targets of atrial fibrillation. PMID: 24301786
  27. biosynthesis of catecholamine by the action of TH should be deeply involved in decreased intellectual ability in patients with schizophrenia PMID: 24417771
  28. A297, E362/E365 and S368 of TH were shown to mediate high affinity dopamine inhibition through V(max) reduction and increasing the K(M) for the cofactor. PMID: 24334288
  29. Tyrosine hydroxylase polymorphisms contribute to attempted suicide in schizophrenia. PMID: 24275212
  30. Neurons of the substantia nigra from the Lesch-Nyhan disease cases show reduced melanization and reduced reactivity for tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine synthesis. PMID: 24891139
  31. Achilles tendon tenocytes produce tyrosine hydroxylase. PMID: 22292987
  32. In a South African cohort, Africans had a higher incidence of hypertension and higher occurrence of the C-824T TH mutation. However, the contribution of the tyrosine hydroxylase C-824T polymorphism to hypertension could not be confirmed. PMID: 23489065
  33. Nurr1 overexpression significantly increased the SIRT1 occupancy of the consensus elements for Nurr1 binding hTH promoter region. PMID: 23977047
  34. The region surrounding pSer19 of Tyrosine hydroxylase adopts an extended conformation in the 14-3-3gamma-bound state, whereas adopts a bent conformation when free in solution. PMID: 24055376
  35. Data suggest that coordination of nitric oxide to Fe(II) in TyrH is directed by presence of tetrahydropterin at active site, binding in a fashion that may be important for directing first step of catalytic cycle toward hydroxylation of tyrosine. PMID: 24168553
  36. In 10 sporadic cases of dopa-responsive dystonia, only two heterozygous tyrosine hydroxylase mutations (Ser19Cys and Gly397Arg) were found in two subjects with unknown pathogenicity. PMID: 23762320
  37. Data indicate that the C-terminal domain was the immunodominant part of tryptophan hydroxylase TPH1, the epitopes of tryptophan hydroxylase TPH2 and tyrosine hydroxylase (TH) were mainly located in the N-terminal regulatory domains. PMID: 23182718
  38. our studies have clearly identified a glucocorticoid-responsive element in a 7 bp AP-1-like motif in the promoter region at -7.24 kb of the human TH gene PMID: 23647419
  39. In severe prolonged fetal hypoxia, there was a striking reduction or absence of tyrosine hydroxylase in all the mesencephalic nuclei. PMID: 23481708
  40. This review discusses the current understandings on the genetic variants in TH and their correlations with Parkinson's disease. PMID: 22583432
  41. This study presented a THD family with predominant myoclonus-dystonia and a new genotype. PMID: 22815559
  42. molecular analysis revealed two novel heterozygous mutations c.636A>C and c.1124G>C in the TH gene PMID: 22691284
  43. mRNA expressions of AQP4 and TH were found to be reduced whereas that of PBP was found to be elevated when compared with those of healthy control samples PMID: 22083667
  44. Data show calbindin (CB)- and tyrosine hydroxylase (TH)-cells were distributed in the three striatal territories, and the density of calretinin (CR) and parvalbumin (PV) interneurons were more abundant in the associative and sensorimotor striatum. PMID: 22272358
  45. Protein levels for tyrosine hydroxylase peaked during the first year of life then gradually declined to adulthood. PMID: 22336227
  46. data indicate that ligand-bound PR-B is recruited to DNA elements in the TH promoter and acts as a transcriptional activator of the TH gene PMID: 21815951
  47. these results suggest that region-specific methylation and methyl-CpG binding domain proteins play important roles in TH gene regulation in neural stem cells. PMID: 22001923
  48. Human RXRalpha interacts with and represses Nurr1-dependent transcriptional activation in tyrosine hydroxylase (TH)-expressing dopaminergic neuronal stem cells in culture, downregulating TH promoter activity. PMID: 22066143
  49. Data indicate that TH gene expression can be regulated by alpha-synuclein (alpha-SYN); further, interference with TH gene expression through elevated levels of alpha-SYN could be associated with dopaminergic neuronal dysfunction. PMID: 21656370
  50. Data from samples of centenarians, nonagenarians and younger controls suggest that the TH01 STR locus exhibits no significant influence on the ability of attaining exceptional old age in Germans. PMID: 21407269

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Involvement in disease
Segawa syndrome autosomal recessive (ARSEGS)
Subcellular Location
Cytoplasm, perinuclear region.
Protein Families
Biopterin-dependent aromatic amino acid hydroxylase family
Tissue Specificity
Mainly expressed in the brain and adrenal glands.
Database Links

HGNC: 11782

OMIM: 191290

KEGG: hsa:7054

STRING: 9606.ENSP00000370571

UniGene: Hs.435609

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