Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
HE; 2b; Hemagglutinin-esterase; HE protein; EC 3.1.1.53; E3 glycoprotein
Species
Bovine coronavirus (strain Mebus) (BCoV) (BCV)
Expression Region
19-424aa
Target Protein Sequence
FDNPPTNVVSHLNGDWFLFGDSRSDCNHVVNTNPRNYSYMDLNPALCDSGKISSKAGNSIFRSFHFTDFYNYTGEGQQIIFYEGVNFTPYHAFKCTTSGSNDIWMQNKGLFYTQVYKNMAVYRSLTFVNVPYVYNGSAQSTALCKSGSLVLNNPAYIAREANFGDYYYKVEADFYLSGCDEYIVPLCIFNGKFLSNTKYYDDSQYYFNKDTGVIYGLNSTETITTGFDFNCHYLVLPSGNYLAISNELLLTVPTKAICLNKRKDFTPVQVVDSRWNNARQSDNMTAVACQPPYCYFRNSTTNYVGVYDINHGDAGFTSILSGLLYDSPCFSQQGVFRYDNVSSVWPLYSYGRCPTAADINTPDVPICVYDPLPLILLGILLGVAVIIIVVLLLYFMVDNGTRLHDA
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant Hemagglutinin-esterase (HE) is a full-length of mature protein derived from the yeast cells. The expression region of this protein is the 19-424aa of Bovine coronavirus (BCoV) HE protein. It carries an N-terminal 6xHis-tag and has a calculated molecular weight of 47.7kDa. Its purity is greater than 90% as determined by SDS-PAGE analysis. In-stock HE proteins are available now. This recombinant HE protein may be used to produce specific antibodies or in the studies of BCoV infection.
The HE protein is one of five major structural proteins of BCoV. It contains two important functional domains: the lectin domain that recognizes sugar receptors in the cell and the esterase domain that bears a receptor‐destroying enzyme activity capable of removing cellular receptors from the surfaces of the targeted cells. Both of these two domains may be critical for virus entry. Therefore, apart from the S protein, the HE protein acts as a second viral attachment protein for infection initiation.
