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| Code | CSB-YP323648BJK |
| Size | $1916 How to order? |
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| Description |
Recombinant Hemagglutinin-esterase (HE) is a full-length of mature protein derived from the yeast cells. The expression region of this protein is the 19-424aa of Bovine coronavirus (BCoV) HE protein. It carries an N-terminal 6xHis-tag and has a calculated molecular weight of 47.7kDa. Its purity is greater than 90% as determined by SDS-PAGE analysis. In-stock HE proteins are available now. This recombinant HE protein may be used to produce specific antibodies or in the studies of BCoV infection. The HE protein is one of five major structural proteins of BCoV. It contains two important functional domains: the lectin domain that recognizes sugar receptors in the cell and the esterase domain that bears a receptor‐destroying enzyme activity capable of removing cellular receptors from the surfaces of the targeted cells. Both of these two domains may be critical for virus entry. Therefore, apart from the S protein, the HE protein acts as a second viral attachment protein for infection initiation. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | HE |
| Uniprot No. | P15776 |
| Research Area | Others |
| Alternative Names | HE; 2b; Hemagglutinin-esterase; HE protein; EC 3.1.1.53; E3 glycoprotein |
| Species | Bovine coronavirus (strain Mebus) (BCoV) (BCV) |
| Source | Yeast |
| Expression Region | 19-424aa |
| Target Protein Sequence | FDNPPTNVVSHLNGDWFLFGDSRSDCNHVVNTNPRNYSYMDLNPALCDSGKISSKAGNSIFRSFHFTDFYNYTGEGQQIIFYEGVNFTPYHAFKCTTSGSNDIWMQNKGLFYTQVYKNMAVYRSLTFVNVPYVYNGSAQSTALCKSGSLVLNNPAYIAREANFGDYYYKVEADFYLSGCDEYIVPLCIFNGKFLSNTKYYDDSQYYFNKDTGVIYGLNSTETITTGFDFNCHYLVLPSGNYLAISNELLLTVPTKAICLNKRKDFTPVQVVDSRWNNARQSDNMTAVACQPPYCYFRNSTTNYVGVYDINHGDAGFTSILSGLLYDSPCFSQQGVFRYDNVSSVWPLYSYGRCPTAADINTPDVPICVYDPLPLILLGILLGVAVIIIVVLLLYFMVDNGTRLHDA Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 47.7kDa |
| Protein Length | Full Length of Mature Protein |
| Tag Info |
N-terminal 6xHis-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.
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| Subcellular Location | Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. |
| Protein Families | Influenza type C/coronaviruses hemagglutinin-esterase family |
