ato Antibody

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Code	CSB-PA342195XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) ato Polyclonal antibody

Uniprot No.

P48987

Target Names

ato

Alternative Names

ato antibody; CG7508Protein atonal antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) ato protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Developmental protein involved in neurogenesis. Required for the formation of chordotonal organs and photoreceptors. Seems to bind to E boxes. Specifically required for the photoreceptor R8 selection.

Gene References into Functions

the regulation of ato expression in the Bolwig's organs primordium, was examined. PMID: 27693434
three binding sites are essential for atonal induction in the posterior ocelli, but show considerable redundancy in the anterior ocellus. this difference parallels the distinct control of ato transcription in the posterior and anterior progenitors of the developing compound eyes. PMID: 27565023
findings suggest a role for the non-bHLH portion of Ato proteins in modulating the function of the bHLH domain in eye neurogenesis and implicate specific aa residues of the bHLH in this process PMID: 21998072
Ato and Sens selectively enhance abdominal SOP development in a Spitz-independent manner, suggesting additional genetic interactions between this proneural pathway and Abd-A. PMID: 20875816
Regulation is disrupted by the rough eye allele of the amos locus. PMID: 11861566
Sensory neurons of the Atonal lineage pioneer the formation of glomeruli within the adult Drosophila olfactory lobe. PMID: 11874920
ato is required for the formation of both the auditory chordotonal sense organ and joint PMID: 12203727
atonal fully rescues the phenotype of Math1 null mice PMID: 12372255
Axonal position in the Drosophila CNS is determined by expression of the Robo3 receptor for the midline repellent Slit. Robo3 expression is dependent on expression of atonal protein. PMID: 12526771
functional comparison of Drosophila atonal, mouse and Xenopus Ath5; findings suggest atonal family may occupy a position in a gene hierarchy where differences in gene regulation or function can be correlated with evolutionary diversity of eye development PMID: 12950631
Regulates DE-cadherin transcriptionally. PMID: 16963016
Kto/Skd act as essential coactivators for Ato expression during early retinal neurogenesis. PMID: 17585897
Despite their high similarity, Ato antagonizes the olfactory promoting ability of Amos protein. PMID: 17709231
Direct control of the proneural gene ato by retinal determination factors during eye development is reported. PMID: 18083159
the regulation of Atonal expression in the developing Drosophila retina. PMID: 18832354
Gain and loss of ato function suppresses and enhances tumor formation, respectively, in a Drosophila cancer. Paradigm PMID: 19243220

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Subcellular Location

Nucleus.

Tissue Specificity

Proneural clusters and sense organ precursors of the chordotonal organs, optic furrow of the eye-antennal disk and developing brain lobe.

Database Links

KEGG: dme:Dmel_CG7508

STRING: 7227.FBpp0081318

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