Neurotensin is a 13-amino acid neuropeptide that functions as both a neurotransmitter and neuromodulator in the central nervous system and as a hormone in the gastrointestinal tract. A larger precursor protein gets cleaved to produce several bioactive peptides including neurotensin, neuromedin N, and large neuromedin N. Neurotensin plays key roles in regulating dopaminergic neurotransmission, pain perception, body temperature, and gastrointestinal motility. The peptide has been linked to various neurological and psychiatric disorders. This makes it a valuable target for research into conditions such as schizophrenia, Parkinson's disease, and substance abuse.
This Human Neurotensin ELISA Kit (CSB-E09144h) uses a quantitative sandwich measurement principle to detect neurotensin in human serum, plasma, and tissue homogenates. The assay provides a detection range of 15.6 pg/ml to 1000 pg/ml with a sensitivity of 3.9 pg/ml. The kit requires 50-100 μl sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, allowing accurate quantification of neurotensin levels for research applications.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in various research contexts to quantify neurotensin levels across different biological sample types. Applications range from cancer cell biology studies examining protein kinase signaling pathways to clinical studies of metabolic and inflammatory biomarkers in human subjects.
• Cancer research: Measurement of neurotensin secretion in lung cancer cell lines following genetic manipulation of signaling proteins to understand cellular regulatory mechanisms
• Clinical biomarker studies: Quantification of neurotensin in human serum alongside inflammatory markers and liver function parameters in patient populations
• Neurological disease research: Analysis of neurotensin peptide levels in brain tumor tissue homogenates to study neuropeptide expression patterns in glioma pathology
