Product Details

Target Name

activity-regulated cytoskeleton-associated protein

Alternative Names

activity regulated cytoskeleton associated protein ELISA Kit; activity regulated gene 3.1 protein homolog ELISA Kit; Activity-regulated cytoskeleton-associated protein ELISA Kit; Activity-regulated gene 3.1 protein homolog ELISA Kit; Arc ELISA Kit; ARC/ARG3.1 ELISA Kit; ARC_HUMAN ELISA Kit; Arg 3.1 ELISA Kit; Arg3.1 ELISA Kit

Abbreviation

ARC

Uniprot No.

Q7LC44

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

25 pg/mL-1600 pg/mL

Sensitivity

6.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	101-113
1:2	Average %	105
	Range %	102-109
1:4	Average %	88
	Range %	85-94
1:8	Average %	95
	Range %	92-98

Recovery

The recovery of human ARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-103
EDTA plasma (n=4)	88	86-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.805	2.720	2.763	2.652
800	1.396	1.515	1.456	1.345
400	0.786	0.842	0.814	0.703
200	0.481	0.471	0.476	0.365
100	0.286	0.275	0.281	0.170
50	0.182	0.186	0.184	0.073
25	0.134	0.132	0.133	0.022
0	0.109	0.112	0.111

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human ARC ELISA Kit was designed for the quantitative measurement of Human ARC protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

1) What are the recommended minimum dilutions for human plasma and serum?
2) Is the kit compatible in human CSF or do you have any data indicating that the kit is capable of detecting ARC protein in CSF?
3) Is the kit compatible with samples from non-human primates? Do you have any data from biofluids in NHPs where the kit has had reactivity with NHP ARC protein?

Thanks for your inquiry!
There is no need to dilute the normal human serum and plasma sample generally.
And I will send you manual reference by email later.
As for the CSF and NHPS samples, so sorry that we have no detection because the samples are difficult to collect. Here advice yo to arrange preliminary experiment.
Appreciated to your kind understanding.

Target Background

Function
(From Uniprot)

Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory. Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1. In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation.

Gene References into Functions

The ARC complex revealed association with verbal and total IQ in child. PMID: 28671113
Study identified several rare mutations that reduced ARC expression in schizophrenia patients. Furthermore, DNA methylation of ARC in schizophrenia patients may be associated with a downregulation of ARC mRNA expression. These findings suggest that multiple rare ARC variants and DNA methylation of ARC might contribute to the pathogenesis of schizophrenia. PMID: 27464451
missense mutations in TRIAD3 abolished the interaction of TRIAD3A with Arc, disrupting Arc ubiquitination, and consequently Arc degradation. PMID: 27995769
ARC confers resistance to sunitinib in renal cell carcinoma through alternate angiogenesis pathways PMID: 26995091
We tested whether ARC variants are associated with six measures of cognitive functioning in 670 healthy subjects in the Norwegian Cognitive NeuroGenetics (NCNG) by extracting data from its Genome-Wide Association Study (GWAS). PMID: 26516611
this is the first report associating an ARC SNP with schizophrenia and supports recent large-scale GWAS findings implicating the ARC complex in schizophrenia risk PMID: 26474411
we propose that the peculiar characteristics of Arc protein, such as its optimal expression after ongoing experience or familiar behavior could explain how familiarization and recognition memories are stored and preserved in the mammalian brain--{REVIEW} PMID: 26380114
mRNA levels of ARC and SGK1 did not differ significantly between the schizophrenia or control samples. PMID: 26038830
Arc is a flexible multi-domain protein that exists in monomeric and oligomeric forms, compatible with a diverse, hub-like role in plasticity-related processes. PMID: 25748042
Degradation of Arc by clathrin-localized Triad3A regulates the availability of synaptic AMPA receptors. PMID: 24945773
we report a novel ARC single nucleotide polymorphism associated with a reduced risk of developing Alzheimer disease. PMID: 22622366
analysis of dynamic, multifaceted control of Arc synthesis during mAchR signaling PMID: 22584581
Strong fibrinogen and Abeta deposition is demonstrated in small- and medium-sized vessels, but not in large cerebral arteries, of 24-month-old transgenic arcAbeta mice. PMID: 22302811
Arc Expression Is Increased in medial frontal cortex of patients with Alzheimer's Disease. PMID: 22036569
Arc/MS2 mRNA assembles into particles that move independently, bidirectionally, and intermittently. These observations identify several phases of Arc mRNA movement that serve as potential points for regulating Arc mRNA localization. PMID: 17120280

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Subcellular Location

Extracellular vesicle membrane; Lipid-anchor. Cell junction, synapse, postsynaptic cell membrane; Lipid-anchor. Cell junction, synapse. Cell junction, synapse, postsynaptic density. Early endosome membrane. Cell projection, dendrite. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Cell projection, dendritic spine. Cytoplasmic vesicle, secretory vesicle, acrosome.

Protein Families

ARC/ARG3.1 family

Database Links

HGNC: 648

OMIM: 612461

KEGG: hsa:23237

STRING: 9606.ENSP00000349022

UniGene: Hs.40888

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Code	CSB-EL001981HU
Size	96T,5×96T,10×96T
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Human Activity-regulated cytoskeleton-associated protein(ARC) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background